Pasteurella multocida pathogenesis: 125 years after Pasteur

Harper, Marina; Boyce, John D.; Adler, Ben

doi:10.1111/j.1574-6968.2006.00442.x

Cited by 356 publications

(326 citation statements)

References 108 publications

(121 reference statements)

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“…No pigs appeared to be coinfected with multiple strains with the locus of the capsule of serogroups A and D in the present study. Serious respiratory symptoms in pig may stem from the more pathogenic virulence factors (hsf-1 and nanB) and the dermonecrotic toxin of serogroup D [8,14]. Therefore, ongoing observation of the pattern of pig respiratory disease is needed.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Characterization and Random Amplified Polymorphic DNA (RAPD) analysis of <i>Pasteurella multocida</i> isolated from Korean Pigs

et al. 2012

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ABSTRACT. Pasteurella multocida causes various respiratory disease symptoms in pigs, including atrophic rhinitis and pneumonia. In the present study, 69 strains of P. multocida were isolated from 443 pigs with respiratory clinical symptoms at 182 farms located throughout South Korea from 2009 to 2010. A multiplex capsular PCR typing assay revealed that 69 strains of P. multocida isolated in this study had the biosynthetic locus of the capsules of either serogroup A (47 strains, 68.1%) or serogroup D (22 strains, 31.9%). The 22 strains positive for serogroup D-specific primers were divided into four clusters and the 47 strains positive for serogroup A-specific primers were divided into 12 clusters according to the results of Random Amplified Polymorphic DNA (RAPD) analysis. P. multocida strains in the present study were susceptible to most of the antimicrobial agents used. An analysis of antimicrobial resistance and virulence gene pattern combined with RAPD indicated that a certain P. multocida strain appeared to be genetically identical, implying the persistence of the strain within a single farm.

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Section: Discussionmentioning

confidence: 99%

Phenotypic Characterization and Random Amplified Polymorphic DNA (RAPD) analysis of <i>Pasteurella multocida</i> isolated from Korean Pigs

et al. 2012

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“…The major virulence factors identified in P. multocida are the capsule protein and lipopolysaccharide (LPS) (Harper et al 2006). However, many other factors may be related, including genes encoding structures such as fimbriae and bacterial adhesins or outer membrane proteins (Corney et al 2007, Hatfaludi et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Detection of virulence-associated genes of Pasteurella multocida isolated from cases of fowl cholera by multiplex-PCR

et al. 2013

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The current systems of breeding poultry, based on high population density, increase the risk of spreading pathogens, especially those causing respiratory diseases and those that have more than one host. Fowl Cholera (FC) is one such pathogen, and even though it represents one of several avian diseases that should be considered in the differential diagnosis of notifiable diseases that present with sudden death, the pathogenesis and virulence factors involved in FC are still poorly understood. The objective of this study was to investigate twelve genes related to virulence in 25 samples of Pasteurella multocida isolated from FC cases in the southern region of Brazil through the development of multiplex PCR protocols. The protocols developed were capable of detecting all of the proposed genes. The ompH, oma87, sodC, hgbA, hgbB, exBD-tonB and nanB genes were present in 100% of the samples (25/25), the sodA and nanH genes were present in 96% (24/25), ptfA was present in 92% (23/25), and pfhA was present in 60% (15/25). Gene toxA was not identified in any of the samples studied (0/25). Five different genetic profiles were obtained, of which P1 (negative to toxA) was the most common. We concluded that the multiplex-PCR protocols could be useful tools for rapid and simultaneous detection of virulence genes. Despite the high frequency of the analyzed genes and the fact that all samples belonged to the same subspecies of P. multocida, five genetic profiles were observed, which should be confirmed in a study with a larger number of samples.

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“…One member of this family, Pasteurella multocida, is the causative agent of multiple different diseases that have great economic impact on animal production, including hemorrhagic septicemia in cattle, atrophic rhinitis in swine and fowl cholera (FC) in domesticated and wild birds (Harper et al, 2006;Glisson, 2008;Rigobelo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods

Furian

Borges

Pilatti

et al. 2014

Rev. Bras. Cienc. Avic.

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The ability of Pasteurella multocida to invade and multiply in its host is enhanced by the presence of the capsule, one of the most important virulence factors for this bacterium. Capsular typing methods are often used in epidemiological and pathogenesis studies of this agent. Five different serogroups have been identified based on serological typing. However, such tests are laborious, and agglutination of homologous antiserum may fail. The aim of this study was to develop a multiplex PCR protocol for the identification of the hyaD-hyaC and dcbF genes specific to serogroups A and D, respectively, and to compare these results with those of phenotypic tests for 54 strains isolated from fowl cholera cases in southern Brazil. The kappa coefficient and chisquare statistics were calculated to assess the agreement between the diagnostic methods and to determine the significance of the results, respectively. The multiplex PCR was able to detect the evaluated genes. Forty-nine strains (90.74%) were classified into serogroup A, and only two isolates (3.7%) were not identified as belonging to any of the serogroups analyzed. In contrast, with the phenotypic tests, only 41 strains (75.93%) were classified into serogroup A and 11 samples (20.37%) were unidentifiable. Of the strains analyzed, 70.37% were classified into the same serogroup (A) by both methods, and the kappa coefficient (k = 0.017) indicated poor agreement between the tests. Thus, multiplex PCR is an alternative for P. multocida capsular typing, as it allows the simultaneous and rapid detection of genes and also provides a greater strain-typing capacity.

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Pasteurella multocida pathogenesis: 125 years after Pasteur

Cited by 356 publications

References 108 publications

Phenotypic Characterization and Random Amplified Polymorphic DNA (RAPD) analysis of <i>Pasteurella multocida</i> isolated from Korean Pigs

Phenotypic Characterization and Random Amplified Polymorphic DNA (RAPD) analysis of <i>Pasteurella multocida</i> isolated from Korean Pigs

Detection of virulence-associated genes of Pasteurella multocida isolated from cases of fowl cholera by multiplex-PCR

Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods

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