Pasteurella multocida infections in mice with reference to haemorrhagic septicaemia in cattle and buffalo

Abstract

Summary Haemorrhagic septicaemia (HS) is an infectious disease of cattle and buffalo caused by particular serotypes of Pasteurella multocida and is one ofthe most economically important livestock diseases in South-East Asia. While HS has been recognized for many years, very little is understood about the disease, primarily because ofthe expense of cattle and a lack of suitable animal models. The suitability of using mice to study HS was assessed using parameters such as the critical pathogenic dose, kinetics of infection, pathology of disease and resistance to reinfection. Pasteurella multocida Ml404. the type strain for Carter group B, the serotype responsible for Asian HS. was injected intraperitoneally into BALB/c mice. As few as 20 colony forming units produced an overwhelming septicaemia in mice in less than 30 h. The kinetics of infection demonstrated a very rapid in viva multiplication rate. There was no evidence of inhibition of bacterial cell growth by natural host defence mechanisms, even with the very small inocula used. The gross pathology ofthe disease in mice was characterized by splenomegaly, lymphadenopathy and petechial haemorrhages similar to that observed in cattle and buffalo with HS. Mice were found to develop a short-lived resistance to reinfection following a primary infection which had been successfully treated with antibiotics. The mouse would seem to provide an ideal tool by which to study HS. but warrant further studies in order to be able to critically assess it as a model for this economically important disease.

Clinico-pathological Features in Mice Following Oral Exposure to Pasteurella Multocida B: 2

“…Oral inoculation of mice with P. multocida type B resulted in development of expected clinical signs as seen in the intraperitoneal inoculation with the same organism as described in an experiment done by Ramdani et al, 1990 andJesse, 2011. The signs that had been observed in the infected group included ruffled fur, laboured breathing, and closure of the eyes together with the eye discharge and less responsiveness.…”

“…Carcasses dumped into rivers, tanks and channels and carried downstream are often incriminated as a likely method of spread of the disease and it is believed that the organism can survive in animal tissues, and perhaps in decomposing carcasses, for a few days (Joseph, 1979;De Alwis, 1999). The commonly used route of inoculation for HS experiment in mice is intraperitoneal (Ramdani et al, 1990). Other routes that are used, less commonly, include subcutaneous and intramuscular (OIE, 2008).…”

Endophytic fungi from tropical ethnoveterinary plants and their antibacterial efficacy against Pasteurella multocida Capsular Type A strain

“…The fungal extracts were found to show better antimicrobial activity than the plant extracts. P. multocida is a dreadful disease of livestock infecting cattles and pigs (Ramdani, Dawkins, Johnson, Spencer, & Adler, 1990). A study on the antibacterial activity of an endophytic fungi, Phoma sp.…”

“…The potency of G. magna against this pathogen observed in the study is attributed to the presence of effective antimicrobial agents in the endophytic fungal extract. Among all the endophytes and plant extracts, the most potent endophyte, G. magna, was then subjected to in vivo assays to assess its efficacy in an animal model, in cognizance to the artificial systemic infection of P. multocida in mice (Ramdani et al, 1990). In vivo antibacterial activity of the extract of G. magna exhibited noteworthy results in terms of low CFU/mL of vital organs, and reduced pathological conditions in liver and spleen in P. multocida infected animals which support the results of in vitro assays.…”

Ultrastructural pathology Associated with Attachment of Pasteurella multocida serotype B: 2 to Tracheal and Pulmonary Endothelial Cells and Tracheal cilia of Mice

“…However, there have been insufficient report on the lesions that may develop when P. multocida serotype B: 2 interact with the host cell in the respiratory system. The suitability of using mice to study HS was suggested by Ramdani (1990).In this study we report the ultrastructural pathology associated with the adherence of P. multocida B: 2 to the trachea and pulmonary endothelial cells and tracheal cilia in mice.…”