Pasteurella multocida capsule: composition, function and genetics

Boyce, John D.; Chung, Jing Y.; Adler, Ben

doi:10.1016/s0168-1656(00)00309-6

Cited by 72 publications

(51 citation statements)

References 38 publications

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“…Functions assigned to the capsule include desiccation resistance, antiphagocytic activity and interaction with the complement system (Boyce et al, 2000). Additionally, there are conflicting reports in the literature regarding the possible role of capsule in the adhesion to host cells and tissues .…”

Section: Introductionmentioning

confidence: 99%

Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods

Furian

Borges

Pilatti

et al. 2014

Rev. Bras. Cienc. Avic.

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The ability of Pasteurella multocida to invade and multiply in its host is enhanced by the presence of the capsule, one of the most important virulence factors for this bacterium. Capsular typing methods are often used in epidemiological and pathogenesis studies of this agent. Five different serogroups have been identified based on serological typing. However, such tests are laborious, and agglutination of homologous antiserum may fail. The aim of this study was to develop a multiplex PCR protocol for the identification of the hyaD-hyaC and dcbF genes specific to serogroups A and D, respectively, and to compare these results with those of phenotypic tests for 54 strains isolated from fowl cholera cases in southern Brazil. The kappa coefficient and chisquare statistics were calculated to assess the agreement between the diagnostic methods and to determine the significance of the results, respectively. The multiplex PCR was able to detect the evaluated genes. Forty-nine strains (90.74%) were classified into serogroup A, and only two isolates (3.7%) were not identified as belonging to any of the serogroups analyzed. In contrast, with the phenotypic tests, only 41 strains (75.93%) were classified into serogroup A and 11 samples (20.37%) were unidentifiable. Of the strains analyzed, 70.37% were classified into the same serogroup (A) by both methods, and the kappa coefficient (k = 0.017) indicated poor agreement between the tests. Thus, multiplex PCR is an alternative for P. multocida capsular typing, as it allows the simultaneous and rapid detection of genes and also provides a greater strain-typing capacity.

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Section: Introductionmentioning

confidence: 99%

Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods

Furian

Borges

Pilatti

et al. 2014

Rev. Bras. Cienc. Avic.

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“…meningitidis (26), and PhyA and PhyB in P. multocida (27). The roles of these proteins in CPS assembly have been debated.…”

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confidence: 99%

KpsC and KpsS are retaining 3-deoxy- d - manno -oct-2-ulosonic acid (Kdo) transferases involved in synthesis of bacterial capsules

Willis

Whitfield

2013

Proc. Natl. Acad. Sci. U.S.A.

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Significance Capsules are bacterial surface structures used by many Gram-negative pathogens to evade the host immune system. They are comprised of long carbohydrate chains, called capsular polysaccharides (CPSs), which possess a lipid at one end. The lipid is connected to the CPS through an unusual linker consisting of five to nine residues of an acidic sugar 3-deoxy- d - manno -oct-2-ulosonic acid (Kdo). This study identifies two conserved proteins from CPS assembly systems, KpsC and KpsS, as the enzymes that synthesize the Kdo linker on the terminal lipid. Synthesis of this terminal glycolipid was reconstituted in vitro using purified enzymes and a synthetic lipid acceptor. The data support a unique model for CPS biosynthesis and identify these enzymes as unique targets for antibacterial therapies.

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“…Overall, region II showed no sequence identity to the previously described specific capsular regions from other H. influenzae serotypes (7,18,19). On the contrary, high overall sequence identity (67%) was found with the capsule biosynthetic-specific region II from Pasteurella multocida B:2 (accession number AF169324), indicating that the genetic organization of the whole region is similar (2,3). The GϩC content of the DNA in the Hie cap locus region II is 31.3%, significantly different from that of both regions I and III (38% and 39.4%, respectively) and from the overall background for the H. influenzae species (38%), suggesting that region II might be more recently acquired.…”

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confidence: 72%

Genetic Characterization of the Capsulation Locus of Haemophilus influenzae Serotype e

et al. 2010

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Pasteurella multocida capsule: composition, function and genetics

Cited by 72 publications

References 38 publications

Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods

Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods

KpsC and KpsS are retaining 3-deoxy- d - manno -oct-2-ulosonic acid (Kdo) transferases involved in synthesis of bacterial capsules

Genetic Characterization of the Capsulation Locus of Haemophilus influenzae Serotype e

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