Pasteurella multocida Bacterial Meningitis in a 33-Day-Old Infant

Slehria, Trisha; Hendricks, Sydney; Honeycutt, Travis

doi:10.1097/inf.0000000000003523

Cited by 5 publications

(3 citation statements)

References 8 publications

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“…While infections caused by P. multocida in humans are well documented ( 14 , 35 – 37 ), little is known about the mechanisms involved. Our recent work revealed that P. multocida isolates from different animals demonstrate the capacities to adhere to and invade human respiratory epithelial cells (BEAS-2B) ( 25 ).…”

Section: Discussionmentioning

confidence: 99%

Vascular Endothelial Growth Factor A Contributes to Increased Mammalian Respiratory Epithelial Permeability Induced by Pasteurella multocida Infection

et al. 2023

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Section: Discussionmentioning

confidence: 99%

Vascular Endothelial Growth Factor A Contributes to Increased Mammalian Respiratory Epithelial Permeability Induced by Pasteurella multocida Infection

et al. 2023

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“…While infections caused by P. multocida in humans is well documented (16,(35)(36)(37), little is known about the mechanisms involved. Our recent work revealed that P. multocida isolates from different animals demonstrate capacities of adhering and invading the human respiratory epithelial cells (BEAS-2B) (20).…”

Section: Discussionmentioning

confidence: 99%

Vascular endothelial growth factor A contributes to the increasing of mammalian respiratory epithelial permeability induced byPasteurella multocidainfection

Lin

Yang

Zhang

et al. 2022

Preprint

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Infections withPasteurella multocidacan cause significant zoonotic respiratory problems in both humans and animals.In vivotests in mouse infection models were used to investigate the mechanisms of respiratory epithelial barrier dysfunction during respiratory bacterial infection with these pathogens. Results revealed thatP. multocidainfection significantly increased epithelial permeability and increased expression of vascular endothelial growth factor A (VEGFA) and endothelial nitric oxide synthase (eNOS) in murine tracheae and lungs. In murine lung epithelial cell (MLE-12) models,P. multocidainfection decreased the expression of tight junctions (ZO-1) and adherens junctions (β-catenin, E-cadherin), but induced the activation of the hypoxia-inducible factor-1α (HIF-1α) and VEGFA signaling. When expression of HIF-1α is suppressed, the induction of VEGFA and ZO-1expression byP. multocidainfection is decreased. We also found that intervention of HIF-1α and VEGFA signaling affected infection outcomes caused by respiratory bacteria in mouse models. Most importantly, we demonstrated thatP. multocidainfection increased permeability of human respiratory epithelial cells and this process was associated with the activation of the HIF-1α and VEGFA signaling and likely contributes to the pathogenesis ofP. multocidain humans.

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“…In addition, Pm can be transmitted by mosquitoes, flies, or blood-sucking insects or by direct bites by cats, dogs, and other animals (2,3). It has even been reported that people might be infected by ill animals in an unknown manner, highlighting its potential threat to human health (4)(5)(6)(7). These diseases have caused huge economic losses to the cattle industry because of increased mortality, high treatment costs, reduced growth rate, and poor carcass quality (8).…”

Section: Introductionmentioning

confidence: 99%

Construction of a one-step multiplex real-time PCR assay for the detection of serogroups A, B, and E of Pasteurella multocida associated with bovine pasteurellosis

et al. 2023

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Bovine pasteurellosis, caused by serogroups A, B, and E of Pasteurella multocida (Pm), is mainly manifested as bovine respiratory disease (BRD) and hemorrhagic septicemia (HS). The disease has caused a great economic loss for the cattle industry globally. Therefore, identifying the Pm serogroups is critical for optimal diagnosis and subsequent clinical treatment and even epidemiological studies. In this study, a one-step multiplex real-time PCR assay was established. Three pairs of specific primers were prepared to detect the highly conserved genomic regions of serogroups A (HyaD), B (bcbD), and E (ecbJ) of Pm, respectively. The results depicted that the method had no cross-reaction with other bovine pathogens (Mannheimia hemolytica, Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, Salmonella Dublin, Mycobacterium paratuberculosis, infectious bovine rhinotracheitis virus, and Mycoplasma bovis). The linear range (107 to 102 copies/μL) showed the R2 values for serogroups A, B, and E of Pm as 0.9975, 0.9964, and 0.996, respectively. The multiplex real-time PCR efficiency was 90.30%, 90.72%, and 90.57% for CartA, CartB, and CartE, respectively. The sensitivity result showed that the serogroups A, B, and E of Pm could be detected to be as low as 10 copies/μL. The repeatability result clarified that an intra-assay and an inter-assay coefficient of variation of serogroups A, B, and E of Pm was < 2%. For the clinical samples, the detection rate was higher than the OIE-recommended ordinary PCR. Overall, the established one-step multiplex real-time PCR assay may be a valuable tool for the rapid and early detection of the serogroups A, B, and E of Pm with high specificity and sensitivity.

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Pasteurella multocida Bacterial Meningitis in a 33-Day-Old Infant

Cited by 5 publications

References 8 publications

Vascular Endothelial Growth Factor A Contributes to Increased Mammalian Respiratory Epithelial Permeability Induced by Pasteurella multocida Infection

Vascular Endothelial Growth Factor A Contributes to Increased Mammalian Respiratory Epithelial Permeability Induced by Pasteurella multocida Infection

Vascular endothelial growth factor A contributes to the increasing of mammalian respiratory epithelial permeability induced byPasteurella multocidainfection

Construction of a one-step multiplex real-time PCR assay for the detection of serogroups A, B, and E of Pasteurella multocida associated with bovine pasteurellosis

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