Past, present and future of diatoms in biosensing

Leonardo, Sandra; Prieto‐Simón, Beatriz; Campàs, Mònica

doi:10.1016/j.trac.2015.11.022

Cited by 50 publications

(35 citation statements)

References 78 publications

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“…These cell wall components have a diverse variety of shapes and structures which serve putative roles in protection, light modification and buoyancy 1 . Additionally these structures are applicable to nanotechnology in fields such as drug delivery 2 , biosensing 3 , and solar cells and batteries 4 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of a New Protein Family Associated With the Silica Deposition Vesicle Membrane Enables Genetic Manipulation of Diatom Silica

Tesson

Lerch

Hildebrand

2017

Sci Rep

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Diatoms are known for their intricate, silicified cell walls (frustules). Silica polymerization occurs in a compartment called the silica deposition vesicle (SDV) and it was proposed that the cytoskeleton influences silica patterning through the SDV membrane (silicalemma) via interactions with transmembrane proteins. In this work we identify a family of proteins associated with the silicalemma, named SAPs for Silicalemma Associated Proteins. The T. pseudonana SAPs (TpSAPs) are characterized by their motif organization; each contains a transmembrane domain, serine rich region and a conserved cytoplasmic domain. Fluorescent tagging demonstrated that two of the TpSAPs were localized to the silicalemma and that the intralumenal region of TpSAP3 remained embedded in the silica while the cytoplasmic region was cleaved. Knockdown lines of TpSAP1 and 3 displayed malformed valves; which confirmed their roles in frustule morphogenesis. This study provides the first demonstration of altering silica structure through manipulation of a single gene.

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Section: Introductionmentioning

confidence: 99%

Characterization of a New Protein Family Associated With the Silica Deposition Vesicle Membrane Enables Genetic Manipulation of Diatom Silica

Tesson

Lerch

Hildebrand

2017

Sci Rep

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“…The most outstanding example are diatoms, single-cell photosynthetic algae, with distinct silica cell walls called frustules, consisting of highly ordered pore structures, species characteristic patterns and hierarchical pore organization with unique mechanical, molecular transport, optical and photonic properties [ 7 , 8 ]. The biosilica of diatoms has currently found attractive applications in optics [ 9 ], photonics [ 10 ], sensing [ 11 ], biosensing [ 12 ], filtration [ 13 ], microfabrications [ 14 , 15 ], protein separation [ 16 ], catalyses [ 17 ] and drug delivery [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Biosilica from Living Diatoms: Investigations on Biocompatibility of Bare and Chemically Modified Thalassiosira weissflogii Silica Shells

et al. 2016

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In the past decade, mesoporous silica nanoparticles (MSNs) with a large surface area and pore volume have attracted considerable attention for their application in drug delivery and biomedicine. Here we propose biosilica from diatoms as an alternative source of mesoporous materials in the field of multifunctional supports for cell growth: the biosilica surfaces were chemically modified by traditional silanization methods resulting in diatom silica microparticles functionalized with 3-mercaptopropyl-trimethoxysilane (MPTMS) and 3-aminopropyl-triethoxysilane (APTES). Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analyses revealed that the –SH or –NH2 were successfully grafted onto the biosilica surface. The relationship among the type of functional groups and the cell viability was established as well as the interaction of the cells with the nanoporosity of frustules. These results show that diatom microparticles are promising natural biomaterials suitable for cell growth, and that the surfaces, owing to the mercapto groups, exhibit good biocompatibility.

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“…Frustules from diatoms have been investigated for their potential in biosensing, by exploiting their photoluminescence properties for gas detection. Frustules from Aulacoseira or Thalassiosira have been showed to be useful to detect trace of pure gas, as for example NO (g) , H 2(g), and NO 2(g) (Bismuto et al, 2008;Leonardo et al, 2016). Since intact biosilica is still not efficient to distinguish substances in very complex mixtures, to increase sensitivity and specific substance identification, frustules were chemically modified on their silanol groups which could increase PL intensity and peak (Medarevic et al, 2016).…”

Section: Potential Applications Of Biosilicamentioning

confidence: 99%

Optical Properties of Nanostructured Silica Structures From Marine Organisms

et al. 2018

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Light is important for the growth, behavior, and development of both phototrophic and autotrophic organisms. A large diversity of organisms used silica-based materials as internal and external structures. Nano-scaled well-organized silica biomaterials are characterized by a low refractive index and an extremely low absorption coefficient in the visible range, which make them interesting for optical studies. Recent studies on silica materials from glass sponges and diatoms, have pointed out very interesting optical properties, such as light waveguiding, diffraction, focusing, and photoluminescence. Light guiding and focusing have been shown to be coupled properties found in spicule of glass sponge or shells of diatoms. Moreover, most of these interesting studies have used purified biomaterials and the properties have addressed in non-aquatic environments, first in order to enhance the index contrast in the structure and second to enhance the spectral distribution. Although there is many evidences that silica biomaterials can present interesting optical properties that might be used for industrial purposes, it is important to emphases that the results were obtained from a few numbers of species. Due to the key roles of light for a large number of marine organisms, the development of experiments with living organisms along with field studies are require to better improve our understanding of the physiological and structural roles played by silica structures.

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Past, present and future of diatoms in biosensing

Cited by 50 publications

References 78 publications

Characterization of a New Protein Family Associated With the Silica Deposition Vesicle Membrane Enables Genetic Manipulation of Diatom Silica

Characterization of a New Protein Family Associated With the Silica Deposition Vesicle Membrane Enables Genetic Manipulation of Diatom Silica

Biosilica from Living Diatoms: Investigations on Biocompatibility of Bare and Chemically Modified Thalassiosira weissflogii Silica Shells

Optical Properties of Nanostructured Silica Structures From Marine Organisms

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