OBJECTIVE: This study investigated the antibacterial action of 30% red propolis, 40% green propolis and 2.5% sodium hypochlorite with irrigation protocols in infected root canals. METHODS: During 60 days, twenty-four root canals were inoculated with E. faecalis. In all experimental groups were performed root canal preparation and two irrigation protocols -passive ultrasonic irrigation and conventional irrigation. In the groups 1, 3, 5 and 7 it were made root canal preparation associated to conventional irrigation with 30% propolis, 40% propolis, 2.5% sodium hypochlorite and distilled water, respectively. In groups 2, 4, 6 and 8 it was made root canal preparation associated with passive ultrasonic irrigation with the solutions described above. The groups 9 and 10 were the controls (negative and positive). Samples of the root canals were collected and immersed in 7 mL of BHI, for 48 hours, at 37潞C. Bacterial growth was analyzed by turbidity of the culture medium. RESULTS: Antibacterial action was observed of 30% red propolis and 2.5% sodium hypochlorite when used passive ultrasonic irrigation and conventional irrigation after 20 minutes, but in 72 hours it was not effective. CONCLUSION: The irrigating agents and protocols were not effective to eliminate the microorganisms of the infected root canals. Clinical Significance: Propolis presents potential for clinical application due to its antimicrobial, anti-inflammatory, antioxidant and low toxicity properties. Key words: Endodontic; Propolis; Sodium hypochlorite A莽茫o antimicrobiana do extrato de pr贸polis vermelho e verde em canais radiculares infectados RESUMO OBJETIVO: Investigar a a莽茫o antibacteriana do pr贸polis vermelhos 30%, do pr贸polis verde 40% e do hipoclorito de s贸dio 2,5% com protocolos de irriga莽茫o em canais radiculares infectados. M脡TODOS: Durante 60 dias, vinte e quatro canais radiculares foram inoculados com E. faecalis. Em todos os grupos experimentais foi realizado o preparo do canal radicular e dois protocolos de irriga莽茫o -irriga莽茫o ultra-s么nica passiva e irriga莽茫o convencional. Nos grupos 1, 3, 5 e 7 foi feito o preparo do canal radicular associado 脿 irriga莽茫o convencional com pr贸polis 30%, pr贸polis 40%, hipoclorito de s贸dio 2,5% e 谩gua destilada, respectivamente. Nos grupos 2, 4, 6 e 8 foi feito o preparo do canal radicular associado 脿 irriga莽茫o ultra-s么nica passiva com as solu莽玫es descritas acima. Os grupos 9 e 10 foram os controles (negativos e positivos). Amostras dos canais radiculares foram coletadas e imersas em 7 mL de BHI, durante 48 horas, a 37潞C. O crescimento bacteriano foi analisado por turva莽茫o do meio de cultura. RESULTADOS: A莽茫o antibacteriana foi observada com pr贸polis vermelho 30% e hipoclorito de s贸dio 2,5% quando utilizado irriga莽茫o ultra-s么nica passiva e irriga莽茫o convencional ap贸s 20 minutos, por茅m, ap贸s 72 horas as subst芒ncias n茫o foram efetivas. CONCLUS脙O: Os agentes de irriga莽茫o e os protocolos n茫o foram efetivos para eliminar os microorganismos dos canais radiculares infectados.