Passive transfer of immunity with serum in mice infected with Nematospiroides dubius: Influence of quality and quantity of immune serum

Dobson, Colin

doi:10.1016/0020-7519(82)90018-2

Cited by 55 publications

(38 citation statements)

References 11 publications

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“…Immunity to N. dubius in mice manifests as loss, stunting and reduced fecundity of adult parasites (21). When affinity fractioned antigens were assayed as candidate vaccines in mice against N. dubius (2 doses IP, 15 ^g IMIgAg in FCA), they conferred up to 57''/o loss and reduced parasite fecundity.…”

Section: Discussionmentioning

confidence: 99%

Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms

Monroy

Dobson

1987

Immunol Cell Biol

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Summary.A crude soluble extract (AWH) obtained from homogenized adult Nematospiraides dubius worms was fractionated into normal (NMtgAg) and immune (IMlgAg) antigens by sequential passage through Sepharose 4B, and CNBr-activaled Sepharose 4B to which immunoglobulins from normal (NMIg) and N. d»ft(U5-infected mouse serum (IMIg) had been coupled. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGF) under reducing condilions showed that MNtgAgand IMtgAg contained several proteins with molecular weights (MW) between 20,000 and 200,0(X). IMtgAg contained 2 polypeptides, MW 24,000 and 55,(XX), which reacted strongly in western bloi analysis against IMig; the larger molecule related lo a cuticular component of the worm. Protective high MW AWH G-200 filtrates shared 5 polypeptides with IMlgAg (MW 85,000-200.000) together with several smaller molecules (MW 30,000-< 10,000). The MW 55,O(X) and 24.0(X) components concentrated in JMlgAg were restricted to non-protective low MW AWH gel fiUraies. BCM mice vaccinated with IMtgAg were protected againsi N. dubius. Worm numbers, size and feciindily were reduced to an extent commensurate with that obtained in mice vaccinated wiih AWH. Mice vaccinaled wiih NMIgAg showed partial resistance. The role of surface and dominant parasite immunogens in immunity is discussed.

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Section: Discussionmentioning

confidence: 99%

Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms

Monroy

Dobson

1987

Immunol Cell Biol

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“…dubius or A^. brasiliensis demonstrable in recipients of serum is presumably mediated by antibodies (Chandler, 1936;Sarles, 1939;Hunter and Leigh, 1961;Jones and Rubin, 1974;Dobson and Owen, 1978;Behnke and Parish, 1979;Miller, 1980, Dobson, 1982.…”

Section: Discussionmentioning

confidence: 99%

“…Reduced faecal epg outptits may reflect the operation of two, perhaps related, immunological events. First, a loss of worms from the infecting larvae and stunting of the survivors (Dobson, 1982;Cayzer and Dobson, 1982;Dobson, Brindley and Sitepu, 1982) and, second, depressed parasite fecundity. Chandler (1936) showed that egg production of A^.…”

Section: Discussionmentioning

confidence: 99%

“…N. brasiliensis antigen was prepared in the same way using adult wcrms collected from 8-day infections in Wistar rats. The determinations of serum anti-parasite antibody titres by indirect haemagglutination assay (IHA) were done using the methods of Dobson (1982).…”

Section: Antigen Preparation and Ihamentioning

confidence: 99%

“…Both rats and mice, respectively, can be passively protected with antiserum against Nippostrongylus brasiliensis and Nematospiroides dubius and in each case both the quantity and quality of the immune serum transferred influenced the degree of protection conferred (Sarles and Taliaferro, 1936;Sarles, 1939;Miller, 1980;Hunter and Leigh, 1961;Jones and Rubin, 1974;Dobson and Owen, 1978;Dobson, 1982).…”

Section: Introductionmentioning

confidence: 99%

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SPECIFICITY OF PASSIVE SERUM PROTECTION AGAINST NIPPOSTRONGYLUS BRASILIENSIS AND NEMATOSPIROIDES DUBIUS IN MICE

Brindley

Dobson

1983

Aust J Exp Biol Med

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Summary Fewer and smaller worms were recovered from, and significantly fewer parasite eggs were voided by, mice injected with mouse antiserum raised against Nippostrongylus brasiliensis or Nematospiroides dubius after infection with the homologous nematode species compared with the control mice. The passive transfer of protective immunity was specific for the species which induced the antibody response in the donor mice. Passively immunized mice infected with the heterologous nematode species harboured the same number of worms, with the same epg output, as parasites in the control mice, but were stunted. Serological cross‐reactions were observed between the two donor antiserum pools and N. dubius and N. brasiliensis antigens.

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Examination of Strategies for Vaccination against Parasitic Infection or Disease Using Mouse Models

Mitchell

Anders

Chapman

et al. 1984

Immunobiology of Parasites and Parasitic Infections

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Passive transfer of immunity with serum in mice infected with Nematospiroides dubius: Influence of quality and quantity of immune serum

Cited by 55 publications

References 11 publications

Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms

Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms

SPECIFICITY OF PASSIVE SERUM PROTECTION AGAINST NIPPOSTRONGYLUS BRASILIENSIS AND NEMATOSPIROIDES DUBIUS IN MICE

Examination of Strategies for Vaccination against Parasitic Infection or Disease Using Mouse Models

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