Passive Immunization with Bovine Milk Containing Antibodies to a Cell Surface Protein Antigen-Glucosyltransferase Fusion Protein Protects Rats against Dental Caries

Mitoma, Morihide; Oho, Takahiko; Michibata, Naoko; Okano, Kaoru; Nakano, Yuichi; Fukuyama, Masafumi; Kinoshita, Toshihiko

doi:10.1128/iai.70.5.2721-2724.2002

Cited by 27 publications

(21 citation statements)

References 27 publications

(21 reference statements)

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“…Recent studies by others using a similar fusion protein, termed "PAcA-GB," have shown that bovine milk containing antibodies against the fusion protein was effective in controlling the recolonization of S. mutans in the oral cavity of humans (37). Furthermore, passive immunization of rats with bovine milk containing antibodies against the PAcA-GB fusion protein was shown to protect against S. mutans induced dental caries in a rat model (25). However, these investigators did not compare the effectiveness of antibodies to the chimeric protein to that of the antibodies to each component.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Immunogenicity of a Genetic Chimeric Protein Consisting of Two Virulence Antigens ofStreptococcus mutansand Protection against Infection

Zhang

Jespersgaard²,

Lamberty-Mallory³

et al. 2002

Infect Immun

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Section: Discussionmentioning

confidence: 99%

Enhanced Immunogenicity of a Genetic Chimeric Protein Consisting of Two Virulence Antigens ofStreptococcus mutansand Protection against Infection

Zhang

Jespersgaard²,

Lamberty-Mallory³

et al. 2002

Infect Immun

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“…Recently, Koga and colleagues (Mitoma et al, 2002) have demonstrated passive protection of rats against dental caries using milk produced from cows vaccinated with a Pac-Gtf fusion protein antigen. This suggests the possibility of using foods containing antibodies as a means of passively protecting children against dental caries.…”

Section: (B) Vaccines Against Oral Diseasesmentioning

confidence: 99%

Molecular Genetic Analysis of the Virulence of Oral Bacterial Pathogens: An Historical Perspective

Kuramitsu

2003

Critical Reviews in Oral Biology & Medicine

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Applying the then-available technology, investigators suggested the important role of sucrose in the cariogenic properties of S. mutans (Loesche, 1986). These organisms were demonstrated to metabolize sucrose, as well as other sugars, rapidly to lactic acid and to convert a portion of the dietary disaccharide to glucan and fructan polymers. Several laboratories purified and characterized the enzymes involved in the synthesis of these polysaccharides (Mooser, 1992). One of the earliest genetic approaches to characterization of the virulence of an oral pathogen was the demonstration, by Tanzer and colleagues (Freedman et al., 1981), that spontaneous or chemically induced MOLECULAR GENETIC ANALYSIS OF THE VIRULENCE OF ORAL BACTERIAL PATHOGENS: AN HISTORICAL PERSPECTIVE Howard K. KuramitsuDepartments of Oral Biology and Microbiology, State University of New York, Buffalo, NY 14214; kuramits@buffalo.edu ABSTRACT: This review will focus on the impact of molecular genetic approaches on elucidating the bacterial etiology of oral diseases from an historical perspective. Relevant results from the pre-and post-recombinant DNA periods will be highlighted, including the roles of gene cloning, mutagenesis, and nucleotide sequencing in this area of research. Finally, the impact of whole-genome sequencing on deciphering the virulence mechanisms of oral pathogens, along with new approaches to control these organisms, will be discussed.

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“…CCL19 is a high-affinity functional ligand for CCR7, the receptor molecule expressed on mature DCs [8,9] . It has been demonstrated that CCL19 and CCR7 play an important role in DC homing to lymphoid tissues [15][16][17][18][19] .…”

Section: Wwwchinapharcom Yan Yh Et Almentioning

confidence: 99%

“…Following intramuscular injection of a plasmid DNA vaccine, the encoded gene is thought to be expressed in transfected myocytes at the site of inoculation [4] , as well as in a small number of dendritic cells (DCs) [5][6][7][8] . DCs capture the antigens expressed by transfected cells, process, and then present them to T cells in secondary lymphoid organs where naive www.chinaphar.com Yan YH et al Acta Pharmacologica Sinica npg T cells are activated [9] . In this process, DCs undergo radical changes from immature phagocytic precursor cells to potent antigen-presenting cells (APCs) and upregulate receptors for constitutive chemokines, such as C-C chemokine receptor type 7 (CCR7) [10,11] .…”

Section: Introductionmentioning

confidence: 99%

Co-delivery of ccl19 gene enhances anti-caries DNA vaccine pCIA-P immunogenicity in mice by increasing dendritic cell migration to secondary lymphoid tissues

Yan

et al. 2013

Acta Pharmacol Sin

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Aim: To investigate how co-delivery of the gene encoding C-C chemokine affected the systemic immune responses to an anti-caries DNA vaccine pCIA-P in mice. Methods: Plasmid encoding CCL19-GFP fusion protein (pCCL19/GFP) was constructed by inserting murine ccl19 gene into GFPexpressing vector pAcGFP1-N1. Chemotactic effect of the fusion protein on murine dendritic cells (DCs) was assessed in vitro and in vivo using transwell and flow cytometric analysis, respectively. BALB/c mice were administered anti-caries DNA vaccine pCIA-P plus pCCL19/GFP (each 100 μg, im) or pCIA-P alone. Serum level of anti-PAc IgG was assessed with ELISA. Splenocytes from the mice were stimulated with PAc protein for 48 h, and IFN-γ and IL-4 production was measured with ELISA. The presence of pCCL19/GFP in spleen and draining lymph nodes was assessed using PCR. The expression of pCCL19/GFP protein in these tissues was analyzed under microscope and with flow cytometry. Results: The expression level of CCL19-GFP fusion protein was considerably increased 48 h after transfection of COS-7 cells with pCCL19/GFP plasmids. The fusion protein showed potent chemotactic activity on DCs in vitro. The level of serum PAc-specific IgG was significantly increased from 4 to 14 weeks in the mice vaccinated with pCIA-P plus pCCL19/GFP. Compared to mice vaccinated with pCIA-P alone, the splenocytes from mice vaccinated with pCIA-P plus pCCL19/GFP produced significantly higher level of IFN-γ, but IL-4 production had no significant change. Following intromuscular co-delivery, pCCL19/GFP plasmid and fusion protein were detected in the spleen and draining lymph nodes. Administration of CCL19 gene in mice markedly increased the number of mature DCs in secondary lymphoid tissues. Conclusion: CCL19 serves as an effective adjuvant for anti-caries DNA vaccine by inducing chemotactic migration of DCs to secondary lymphoid tissues.

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Passive Immunization with Bovine Milk Containing Antibodies to a Cell Surface Protein Antigen-Glucosyltransferase Fusion Protein Protects Rats against Dental Caries

Cited by 27 publications

References 27 publications

Enhanced Immunogenicity of a Genetic Chimeric Protein Consisting of Two Virulence Antigens ofStreptococcus mutansand Protection against Infection

Enhanced Immunogenicity of a Genetic Chimeric Protein Consisting of Two Virulence Antigens ofStreptococcus mutansand Protection against Infection

Molecular Genetic Analysis of the Virulence of Oral Bacterial Pathogens: An Historical Perspective

Co-delivery of ccl19 gene enhances anti-caries DNA vaccine pCIA-P immunogenicity in mice by increasing dendritic cell migration to secondary lymphoid tissues

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