Passive immunisation against experimental salmonellosis in mice by orally administered hen egg-yolk antibodies specific for 14-kDa fimbriae of Salmonella Enteritidis

Abstract

Summary. Chickens were immunised with a preparation of purified 14-kDa fimbriae of Salmonella serotype Enteritidis (SEF 14) to raise egg-yolk antibodies for protection trials in mice against subsequent challenge-exposure with the homologous strain of Enteritidis. A pronounced specificity of egg-yolk antibodies against the 14-kDa fimbrial antigen was demonstrated by Western blotting analysis. Passive antibody protection was evaluated in a mouse model of experimental salmonellosis: 79 mice (CD 1 strain) were challenged orally with 2 x 1O1O cfu of Enteritidis. Test mice treated with SEF-14 antibodies (titre = 128) had a survival rate of 77.8% compared to 32% survival in control mice fed normal egg-yolk antibodies (titre < 10) (p < 0.01). In-vitro adhesion of Enteritidis to mouse intestinal epithelial cells was reduced by anti-fimbrial antibodies. An indirect immunofluorescence method demonstrated the localisation of Enteritidis along the villous margins of the small intestine of control mice, whereas in test mice adherent bacteria were not detected. Results suggest that 14-kDa fimbriae may influence, enhance or contribute to the overall adhesive properties of Enteritidis and that egg-yolk antibodies directed against these fimbriae may have played a substantial role in protection, possibly by minimising bacterial colonisation and invasion during the early stages of infection.

Blockade ofSalmonella enteritidisPassage across the Basolateral Barriers of Human Intestinal Epithelial Cells by Specific Antibody

“…Milk immunoglobulins obtained from cows immunized with various pathogens, for example, have been reported to be useful for preventing infectious gastrointestinal diseases and are expected to be good ingredients for milk formulae having a preventative function against infectious diarrhea (22). Recently, attention has been focused on egg yolk immunoglobulin G obtained from immunized hens as another antibody source because of its high productivity (2,18,19,29,30,35,36).…”

“…The protection against infectious diseases provided by these antibodies has been considered to be due to the inhibition of viral or bacterial adherence to the enterocyte (19,30,36). However, it is not clear whether the antibodies act only to inhibit bacterial adherence.…”

Hemagglutinating properties of Salmonella enterica serovar Enteritidis isolated from different sources

“…In S. Enteritidis, several fimbrial hemagglutinins have been implicated in bacterial attachment, colonization and pathogenicity. SEF14 has been shown to contribute to bacterial adherence to mouse epithelial cells and to chicken ovarian granulosa cells (20,28). In addition, SEF 14 may be required for systemic infections (8,21 Our findings indicate that S. Enteritidis produces a nonfimbrial mannose-sensitive hemagglutinin.…”

Scientific Opinion on the public health risks of table eggs due to deterioration and development of pathogens

“…It was suggested that gene loss may be a mechanism of targeting the invading pathogen preferentially to particular tissues or host cells, and can also influence the ability of the pathogen to survive in the external environment or even in stressful situations within the host (Thomson et al, 2008;Feng et al, 2013). The enhanced invasion of the reproductive tract and survival in the forming egg of S. Enteritidis and S. Gallinarum has been linked to the presence of SEF-14 fimbriae ( (Peralta et al, 1994;Thiagarajan et al, 1996;Rajashekara et al, 2000;Rank et al, 2009). S. Enteritidis can colonize the intestinal epithelium of chickens and is then able to reach a variety of internal organ sites, notably the ovaries (Gast and Beard, 1990;Gast, 1994;Peralta et al, 1994;Thiagarajan et al, 1996;Rajashekara et al, 2000;Rank et al, 2009;Berghaus et al, 2011).…”

“…The enhanced invasion of the reproductive tract and survival in the forming egg of S. Enteritidis and S. Gallinarum has been linked to the presence of SEF-14 fimbriae ( (Peralta et al, 1994;Thiagarajan et al, 1996;Rajashekara et al, 2000;Rank et al, 2009). S. Enteritidis can colonize the intestinal epithelium of chickens and is then able to reach a variety of internal organ sites, notably the ovaries (Gast and Beard, 1990;Gast, 1994;Peralta et al, 1994;Thiagarajan et al, 1996;Rajashekara et al, 2000;Rank et al, 2009;Berghaus et al, 2011). Studies have shown that some S. Enteritidis phage types (PTs) are more invasive than others (Hinton et al, 1990;Roy et al, 2001), but other similarly designed studies could not identify any difference in the invasiveness or colonisation ability between different phage types (Barrow, 1991).…”