Passive hemagglutination test for detection of antibodies against Taylorella (Haemophilus) equigenitalis in Sera of Mares

Eguchi, Masashi; Kuniyasu, C; Kishima, Masato

doi:10.1016/0378-1135(88)90061-2

Cited by 4 publications

(3 citation statements)

References 2 publications

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“…Single-step PCR could detect T. equigenitalis from not only 2 samples that were positive by bacterial culture but also from 10 samples that were negative by bacterial culture, suggesting that the PCR test was more sensitive than bacterial isolation from field samples. The 10 bacterial culture negative PCR-positive samples were collected from 5 mares, of which two were positive by bacterial culture and one was positive by serologic examination [8] performed two years prior. Nevertheless, two of the PCRpositive mares appeared to be negative by previous bacteriologic and serologic analyses.…”

Section: Optimization Of Primer Sets For Pcr Testmentioning

confidence: 99%

Development of a PCR Test for Rapid Diagnosis of Contagious Equine Metritis.

et al. 1999

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ABSTRACT. In order to establish a rapid diagnostic method for contagious equine metritis (CEM), we developed and evaluated a polymerase chain reaction (PCR) test. Species-specific PCR primer sets were derived from the DNA sequence of a cloned DNA fragment of Taylorella equigenitalis that did not hybridize with the genome of a taxomonically related species, Oligella urethralis. Single step PCR with primer set P1-N2 and two-step semi-nested PCR with primer sets P1-N2 and P2-N2 detected as low as 100 and 10 CFU of the bacteria, respectively. Single-step PCR detected T. equigenitalis from genital swabs of experimentally infected mares with sensitivity comparable to that of bacterial isolation. Furthermore, two-step PCR was more sensitive than the culture method. Upon examination of field samples, 12 out of 3,123 samples were positive by single-step PCR while only 2 were positive by bacterial culture. The 12 PCRpositive samples originated from 5 mares, of which 3 animals were considered to be carriers based on previous bacteriologic and serologic diagnoses for CEM. The PCR test described in this study would provide a specific and highly sensitive tool for the rapid diagnosis of CEM.-KEY WORDS: contagious equine metritis, equine, metritis, polymerase chain reaction, Taylorella equigenitalis.

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Section: Optimization Of Primer Sets For Pcr Testmentioning

confidence: 99%

Development of a PCR Test for Rapid Diagnosis of Contagious Equine Metritis.

et al. 1999

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“…The current diagnosis of CEM is based on the isolation of T. equigenitalis by conventional bacteriological culture from field genital swabs, the employment of PCR tests [5][6][7][8][9][10] and serology using both polyclonal and monoclonal antibody methodologies [11][12][13][14] directed to protein rather than to specific glycosyl derivatives.…”

Section: Introductionmentioning

confidence: 99%

The structure of the polysaccharide of the lipopolysaccharide produced by Taylorella equigenitalis type strain (ATCC 35865)

Vinogradov

MacLean

Brooks

et al. 2008

Carbohydrate Research

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“…Since then, bacterial isolation tests have been conducted on all breeding Thoroughbreds before the breeding season every year, and surveillance by isolation testing combined with serological testing using a passive hemagglutination test [6] is conducted during the breeding season. On the basis of these tests, the movement and mating of positive horses have been limited.…”

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Contagious Equine Metritis Eradicated from Japan

Anzai

Kamada

Niwa

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. Contagious equine metritis (CEM), a contagious venereal disease of horses, invaded Japan in 1980 and spread in the Thoroughbred population of the Hidaka-Iburi district of Hokkaido. To eradicate CEM, we ran a program aimed at detecting Taylorella equigenitalis, the causal agent, in carrier horses by using the PCR test, followed by culling or treatment. In 2001, the first year of the program, 12,356 Thoroughbred racing stallions and mares were tested and 11 carriers were found. Four, two, one, and one carrier mares were detected in 2002, 2003, 2004, and 2005, respectively

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Passive hemagglutination test for detection of antibodies against Taylorella (Haemophilus) equigenitalis in Sera of Mares

Cited by 4 publications

References 2 publications

Development of a PCR Test for Rapid Diagnosis of Contagious Equine Metritis.

Development of a PCR Test for Rapid Diagnosis of Contagious Equine Metritis.

The structure of the polysaccharide of the lipopolysaccharide produced by Taylorella equigenitalis type strain (ATCC 35865)

Contagious Equine Metritis Eradicated from Japan

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