Passive diffusion of polymeric surfactants across lipid bilayers

Mathot, Frédéric; Schanck, André; Bambeke, Françoise Van; Ariën, Albertina; Noppe, M.; Brewster, Marcus E.; Préat, Véronique

doi:10.1016/j.jconrel.2007.03.015

Cited by 23 publications

(20 citation statements)

References 36 publications

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“…The enhancement in the uptake as a result of the targeting ligand, glucose, at the concentration of Fe at 25 and 100 ppm was 27-fold and fivefold, respectively. The difference of cell uptake between Glu-SPIO micelles and SPIO micelles of 100 ppm was lower than 25 ppm which was probably due to the internalization of micelles by diffusion mechanism (Mathot et al 2007) or nonspecific uptake mechanism of micelles (Savić et al 2003). This mechanism was pronounced at high concentration of micelles.…”

Section: Cellular Uptake Studymentioning

confidence: 87%

Glucose-installed, SPIO-loaded PEG-b-PCL micelles as MR contrast agents to target prostate cancer cells

2017

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Polymeric micelles of poly(ethylene glycol)-block-poly(e-caprolactone) bearing glucose analog encapsulated with superparamagnetic iron oxide nanoparticles (Glu-SPIO micelles) were synthesized as an MRI contrast agent to target cancer cells based on high-glucose metabolism. Compared to SPIO micelles (non-targeting SPIO micelles), Glu-SPIO micelles demonstrated higher toxicity to human prostate cancer cell lines (PC-3) at high concentration. Atomic absorption spectroscopy was used to determine the amount of iron in cells. It was found that the iron in cancer cells treated by Glu-SPIO micelles were 27-fold higher than cancer cells treated by SPIO micelles at the iron concentration of 25 ppm and fivefold at the iron concentration of 100 ppm. To implement Glu-SPIO micelles as a MR contrast agent, the 3-T clinical MRI was applied to determine transverse relaxivities (r 2 *) and relaxation rate (1/T 2 *) values. In vitro MRI showed different MRI signal from cancer cells after cellular uptake of SPIO micelles and Glu-SPIO micelles. Glu-SPIO micelles was highly sensitive with the r 2 * in agarose gel at 155 mM -1 s -1. Moreover, the higher 1/T 2 * value was found for cancer cells treated with Glu-SPIO micelles. These results supported that glucose ligand increased the cellular uptake of micelles by PC-3 cells with over-expressing glucose transporter on the cell membrane. Thus, glucose can be used as a small molecule ligand for targeting prostate cancer cells overexpressing glucose transporter.

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Section: Cellular Uptake Studymentioning

confidence: 87%

Glucose-installed, SPIO-loaded PEG-b-PCL micelles as MR contrast agents to target prostate cancer cells

2017

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“…However, the PAMPA assay has found so far greater acceptance for gastrointestinal tract passive transport prediction, since a broader spectrum of nanomedicines has already been tested, such as polymeric micelles [128], [129,130], liposomes [131,132], solid lipid nanoparticles [133] and solid self-nanoemulsifying drug delivery systems [134,135]. Overall, results pointed out that inclusion in nanocarriers may significantly alter the passive diffusion pattern of the free drug.…”

Section: Model Configurationmentioning

confidence: 99%

In vitro screening of nanomedicines through the blood brain barrier: A critical review

2016

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“…Pluronic 85 unimers enter epithelial cells through caveolae-dependent and independent-pathways whereas micelles are internalized exclusively through CME [59]. PEG-p(CL-co-TMC) unimers can diffuse passively through model lipid bilayers (PAMPA) [65] whereas micelles are taken up by endocytosis [66].…”

Section: Mechanisms Of Cellular Uptake Of Nanocarriersmentioning

confidence: 99%