Passive accumulation of magnesium, sodium, and potassium by chick calvaria

Brommage, Robert; Neuman, W. F.

doi:10.1007/bf02441218

Cited by 13 publications

(8 citation statements)

References 19 publications

(19 reference statements)

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“…The common impurity is carbonate in place of the phosphate groups [13]. Other known substitutions include potassium, magnesium, strontium and sodium in place of the calcium ions, and chloride and fluoride in place of the hydroxyl groups [14-16]. These impurities are believed to decrease the crystallinity of the bone mineral and thus may change certain mineral properties such as solubility, which is important for mineral homeostasis and bone adaptation [1,17].…”

Section: Composition Of Bonementioning

confidence: 99%

Chemical and Biochemical Basis of Cell-Bone Matrix Interaction in Health and Disease

Xing¹

2009

CCB

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Bone, a calcified tissue composed of 60% inorganic component (hydroxyapatite), 10% water and 30% organic component (proteins), has three functions: providing mechanical support for locomotion, protecting vital organs, and regulating mineral homeostasis. A lifelong execution of these functions depends on a healthy skeleton, which is maintained by constant bone remodeling in which old bone is removed by the bone-resorbing cell, osteoclasts, and then replaced by new bone formed by the bone-forming cell, osteoblasts. This remodeling process requires a physical interaction of bone with these bone cells. Moreover, numerous cancers including breast and prostate have a high tendency to metastasize to bone, which is in part attributable to the capacity of the tumor cells to attach to bone. The intensive investigation in the past two decades has led to the notion that the cell-bone interaction involves integrins on cell surface and bone matrix proteins. However, the biochemical composition of bone and emerging evidence are inconsistent with this belief. In this review, I will discuss the current understanding of the molecular mechanism underlying the cell-bone interaction. I will also highlight the facts and new findings supporting that the inorganic, rather than the organic, component of bone is likely responsible for cellular attachment.

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Section: Composition Of Bonementioning

confidence: 99%

Chemical and Biochemical Basis of Cell-Bone Matrix Interaction in Health and Disease

Xing¹

2009

CCB

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“…This practice is not uncommon [11] when, for the sake of simplicity, one wishes to eliminate the unknown complications of media that contain a few too many organic and biological constituents such as serum, hormones, and antibiotics [12,13].…”

Section: Culture Mediamentioning

confidence: 98%

“…The controls were placed in a separate compartment of the incubator, out of the influence of the field. Because of the short-term nature of the culture, no special CO 2 gas composition was used except for the air flow of the incubator itself; this practice is not uncommon [11].…”

Section: Culture Techniquementioning

confidence: 99%

Electromagnetic modulation of biological processes: Influence of culture media and significance of methodology in the Ca-uptake by embryonal chick tibiain vitro

Colacicco

Pilla

1984

Calcif Tissue Int

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The present studies are aimed at establishing molecular correlations in the interaction of very low frequency electromagnetic fields with biological systems. Ca-uptake by chick embryo tibia rudiment in short-term culture was a useful model. Tibiae of 8- to 10-day-old chick embryos were incubated 60 min in simplified culture media in the presence of 45Ca at 37.5 +/- 0.5 degrees C either inside or outside pulsating electromagnetic fields. Radioactivity count in the medium was the most accurate method for determining Ca-uptake by the rudiment. The effect of the fields on the Ca-uptake depended markedly on the chemical composition of the culture medium: bicarbonate was indispensable; glucose or sucrose was important; phosphate was potentiating; ethanol, Mg2+, and NaF were stimulating. The field had no effect in (a) blank medium without tibia, (b) tibiae that had been altered by fixation with aqueous glutaraldehyde, (c) nonliving artificial systems endowed with great or small ion sorption capacity. The unique bicarbonate effect with living systems and the passive behavior of nonliving ion sorbing systems prompt the suggestion that the electromagnetic field probably couples with specific processes, such as a bicarbonate-dependent Ca2+ ATPase and the active ion transport, at the cell membrane level. The molecular mechanisms remain to be established.

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“…The common impurity is carbonate in place of the phosphate groups [13]. Other known substitutions include potassium, magnesium, strontium and sodium in place of the calcium ions, and chloride and fluoride in place of the hydroxyl groups [14][15][16]. These impurities are believed to decrease the crystallinity of the bone mineral and thus may change certain mineral properties such as solubility, which is important for mineral homeostasis and bone adaptation [1,17].…”

Section: Composition Of Bonementioning

confidence: 99%

Chemical and Biochemical Basis of Cell-Bone Matrix Interaction in Health and Disease

2009

Current Chemical Biology

View full text Add to dashboard Cite

Bone, a calcified tissue composed of 60% inorganic component (hydroxyapatite), 10% water and 30% organic component (proteins), has three functions: providing mechanical support for locomotion, protecting vital organs, and regulating mineral homeostasis. A lifelong execution of these functions depends on a healthy skeleton, which is maintained by constant bone remodeling in which old bone is removed by the bone-resorbing cell, osteoclasts, and then replaced by new bone formed by the bone-forming cell, osteoblasts. This remodeling process requires a physical interaction of bone with these bone cells. Moreover, numerous cancers including breast and prostate have a high tendency to metastasize to bone, which is in part attributable to the capacity of the tumor cells to attach to bone. The intensive investigation in the past two decades has led to the notion that the cellbone interaction involves integrins on cell surface and bone matrix proteins. However, the biochemical composition of bone and emerging evidence are inconsistent with this belief. In this review, I will discuss the current understanding of the molecular mechanism underlying the cellbone interaction. I will also highlight the facts and new findings supporting that the inorganic, rather than the organic, component of bone is likely responsible for cellular attachment.

show abstract

Passive accumulation of magnesium, sodium, and potassium by chick calvaria

Cited by 13 publications

References 19 publications

Chemical and Biochemical Basis of Cell-Bone Matrix Interaction in Health and Disease

Chemical and Biochemical Basis of Cell-Bone Matrix Interaction in Health and Disease

Electromagnetic modulation of biological processes: Influence of culture media and significance of methodology in the Ca-uptake by embryonal chick tibiain vitro

Chemical and Biochemical Basis of Cell-Bone Matrix Interaction in Health and Disease

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