Passage of circulating adrenaline into perfused cerebral ventricles and subarachnoidal space

Draskoci, M; Feldberg, W.; Haranath, P. S. R. K.

doi:10.1113/jphysiol.1960.sp006371

Cited by 30 publications

(5 citation statements)

References 18 publications

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“…This recalls the finding of Draskoci (1960), that during the intravenous infusion of 10 [tg/kg/min lysergic acid diethylamide there was no obvious difference in the output of the diethylamide from the perfused cerebral ventricles, whether the perfusion was from lateral ventricle to cisterna or from lateral ventricle to aqueduct, that is, included or excluded part of the subarachnoid space. Draskoci, Feldberg & Haranath (1960) showed, in experiments similar to ours, that during intravenous infusion of adrenaline 40 pg/kg/min, more of the amine appeared in the effluent from the perfused subarachnoid space than from the perfused cerebral ventricles.…”

Section: Discussionsupporting

confidence: 86%

Passage of Intravenously Infused Atropine Into Perfused Cerebral Ventricles and Subarachnoid Space

Haranath

Premalatha

Sunanda-Bai

1966

British Journal of Pharmacology and Chemotherapy

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Stern & Gautier (1921) detected atropine in the cerebrospinal fluid of the dog and of the rabbit, after its intravenous or intracarotid injection. The present experiments were undertaken to see if in the cat also atropine passes from the blood stream into the cerebrospinal fluid, and if so, to determine the rate of its output in the effluents obtained on perfusion of various parts of the cerebral ventricles and subarachnoid space.Our experiments with cats show that atropine, during its intravenous infusion, appears in the cerebrospinal fluid, and further it was found in the effluents from both the perfused cerebral ventricles and the perfused subarachnoid space. There was no significant difference between the output from the cerebral ventricles and that from the subarachnoid space. METHODSCats weighing 1.5 to 4 kg were anaesthetized with chloralose, 70 mg/kg, injected intravenously under ethyl chloride and-ether anaesthesia. The trachea was cannulated. For the atropine infusion a cannula was inserted in the right femoral vein. The atropine sulphate in 0.9% saline was infused at 0.2 ml./min with a continuous slow injector at rates of 1, 10 or 25 pLg/kg/min. Blood samples were obtained from the left femoral artery, which was dissected free and clamped; an opening was made in the artery for the insertion at intervals of 1 hr of a polythene tube, through which the samples were collected into tubes containing heparin, and then immediately centrifuged and the plasma separated. The plasma was kept at room temperature (25 to 300 C) if assayed on the same day, or in the refrigerator (at 4' C) for assay on the next day.Collection of cerebrospinal fluid. A cisternal cannula similar to one described by Bhawe (1958) was inserted into the cisterna magna. The cannula consisted of a 20 S.W.G. hypodermic needle with the butt removed and a small length of polythene tube attached. The free end of the polythene tube was closed with a small glass stillete. Cerebrospinal fluid samples were collected every hr by removing the glass stillete and allowing 0.5 to I ml. of cerebrospinal fluid to flow into a test tube. The stillete was then replaced.

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Section: Discussionsupporting

confidence: 86%

Passage of Intravenously Infused Atropine Into Perfused Cerebral Ventricles and Subarachnoid Space

Haranath

Premalatha

Sunanda-Bai

1966

British Journal of Pharmacology and Chemotherapy

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“…Epinephrine also passes into the brain and is absorbed into the general circulation when injected into the CSF (83). This observation becomes in creasingly interesting in the light of observations made by Titus and co workers that slices of cerebral cortex, when incubated in a medium contain ing the related catecholamine, norepinephrine, take up this compound by a process that has many characteristics of active transport (84).…”

Section: Drug Exchange Between Blood Cerebrospinal Fluid and Brainmentioning

confidence: 95%

Mechanisms of Drug Absorption and Excretion Passage of Drugs in and out of the Central Nervous System

Rall¹,

Zubrod²

1962

Annu. Rev. Pharmacol.

144

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“…In previous experiments in which adrenaline was infused intravenously into anaesthetized cats (Draskoci, Feldberg & Haranath, 1960) or atropine into anaesthetized dogs (Haranath, et ad., 1966) whilst the liquor space was perfused from lateral ventricle to either aqueduct or cisterna magna the concentration of these substances was greater in the cisternal than in the aqueductal effluent. The results of the present experiments are not so clear-cut.…”

Section: Discussionmentioning

confidence: 99%

Passage of intravenously administered tubocurarine into the liquor space in man and dog

Devasankaraiah

Haranath

Krishnamurty

1973

British J Pharmacology

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Summary1. In anaesthetized patients under controlled respiration, samples of lumbar cerebrospinal fluid were withdrawn 15 and 60 min after an intravenous injection of 30 mg tubocurarine. When tested on the frog rectus muscle preparation contracted by acetylcholine, they exerted curare-like activity which corresponded to between 0 05 and 0-33 ,ug/ml tubocurarine. 2. In dogs anaesthetized with pentobarbitone sodium and artificially ventilated, two procedures were adopted to find out if tubocurarine passes into the liquor space after an intravenous injection of 0-3 or 3 mg/kg and during its intravenous infusion at a rate of 10 (jug/kg)/minute. Either samples of cisternal cerebrospinal fluid (c.s.f.) were collected, or different regions of the liquor space were perfused with artificial c.s.f. and the effluent was collected. The samples of c.s.f. and the effluent were assayed for curare-like activity on the frog rectus muscle. 3. After the intravenous injection of tubocurarine samples of cisternal effluent collected during perfusion from lateral ventricle to cisterna exerted curare-like activity. It corresponded to 20 ng/min tubocurarine in the sample collected during the first 15 min after the injection of 0 3 mg/kg and to 40-60 ng/min in the samples collected up to 2 h after the injection of 3 mg/kg. 4 During intravenous infusion of tubocurarine the cisternal c.s.f. as well as the effluent from the perfused regions of the liquor space exhibited curare-like activity. Expressed in equivalents of tubocurarine, the activity in the cisternal c.s.f. ranged from between 0-1 and 0-75 ,ug/ml. On perfusion from lateral ventricle to aqueduct or cistema, the activity ranged from between 3 and 25 ng/min in the aqueductal and from between 4 and 40 ng/min in the cisternal effluent. On perfusion from the lumbar-spinal subarachnoid space to cisterna it ranged from between 6 and 55 ng/min in the cisternal effluent.

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Passage of circulating adrenaline into perfused cerebral ventricles and subarachnoidal space

Cited by 30 publications

References 18 publications

Passage of Intravenously Infused Atropine Into Perfused Cerebral Ventricles and Subarachnoid Space

Passage of Intravenously Infused Atropine Into Perfused Cerebral Ventricles and Subarachnoid Space

Mechanisms of Drug Absorption and Excretion Passage of Drugs in and out of the Central Nervous System

Passage of intravenously administered tubocurarine into the liquor space in man and dog

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