Paspalum

Williams, W. M.; Williamson, M. L.; Real, Daniel

doi:10.1007/978-3-642-14255-0_12

Cited by 5 publications

(2 citation statements)

References 112 publications

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“…241878, tetraploid population) (Fachinetto, Dall'Agnol, Schifino‐Wittmann, Simioni, & Avila, ) and P. juergensii (Accession no. 508779, diploid) (Williams, Williamson, & Real, ) were acquired from the United States Department of Agriculture (USDA) National Plant Germplasm System (https://www.ars-grin.gov/npgs/). Seeds were germinated on moist filter paper in Petri dishes and then transferred to 10‐cm plastic pots containing a mixture (2:1) of Metro‐mix 360 and all‐purpose sand with soil collected from a nearby tallgrass prairie added at 10% by volume to provide a source of natural soil microbial diversity.…”

Section: Methodsmentioning

confidence: 99%

Comparing control options for time‐series RNA sequencing experiments in nonmodel organisms: An example from grasses

Qiu

Bachle

Nippert

et al. 2020

Molecular Ecology Resources

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RNA sequencing (RNA‐seq) is a widely used approach to investigate gene expression and increasingly is used in time‐course studies to characterize transcriptomic changes over time. Two primary options are available as controls in time‐course experiments: samples collected at the first sampling time are used as controls (temporal control, TC) and samples collected in parallel at each individual sampling time are used as controls (biological control, BC). While both approaches are used in experimental studies, we know of no analyses performed to date that directly compare effects of control type choices on identifying differentially expressed genes (DEGs) and subsequent functional analysis. In the current study, we compare experimental results using these different control types for time‐course RNA‐seq drought stress experiments in two wild grass species in the genus Paspalum. Our results showed BC assemblies gave a higher number of loci in both species. The number of DEGs increased with increasing stress and then decreased dramatically at the recovery time point using both control types. Expression levels of the same DEGs were highly correlated between control types in both species, ranging from r = .653 to r = .852. We also observed similar rank orders of shared enriched Gene Ontology term lists using the two different control types. Collectively, our findings suggest similar results in differential gene expression and functional annotation between control types. The ultimate choice of control type will rely on the experimental length and organism type, with labour time and sequencing costs as additional factors to be considered.

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Section: Methodsmentioning

confidence: 99%

Comparing control options for time‐series RNA sequencing experiments in nonmodel organisms: An example from grasses

Qiu

Bachle

Nippert

et al. 2020

Molecular Ecology Resources

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“…flavescens , P. dilatatum “Virasoro”, and P. dilatatum “Vacaria”), one is apomictic pentaploid ( P. dilatatum ssp. dilatatum ), and three are apomictic hexaploid ( P. dilatatum “Chirú”, “Uruguaiana”, and “Torres”) (Burson et al, 1991; Evers and Burson, 2004; Williams et al, 2011). The predominant biotype is the apomictic pentaploid, which is widespread in South America and naturalized in other parts of the world (Evers and Burson, 2004).…”

Section: Population Diversity and Germplasm Collectionmentioning

confidence: 99%

Reproductive Systems in Paspalum: Relevance for Germplasm Collection and Conservation, Breeding Techniques, and Adoption of Released Cultivars

et al. 2019

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The objective of this review is to analyze and describe the impact that mode of reproduction in Paspalum has on germplasm conservation, genetic improvement, and commercialization of cultivars. Germplasm collection and conservation can now be rethought considering the newly available information related to how diversity is allocated in nature and how it can be transferred between the sexual and apomictic germplasm using novel breeding approaches. An inventory of species and accessions conserved around the world is analyzed in relation to the main germplasm banks. Because of the importance of apomixis in Paspalum species different breeding approaches have been used and tested. Knowledge related to the inheritance of apomixis, variable expressivity of the trait and techniques for early identification of apomicts has helped to improve the efficiency of the breeding methods. Novel breeding techniques are also being developed and are described regarding its advantages and limitations. Finally, the impact of reproductive mode on the adoption of the released cultivars is discussed.

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How does drought affect native grasses’ photosynthesis on the revegetation of iron ore tailings?

Rios

Siqueira-Silva

Pereira

2020

Environ Sci Pollut Res

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Paspalum

Cited by 5 publications

References 112 publications

Comparing control options for time‐series RNA sequencing experiments in nonmodel organisms: An example from grasses

Comparing control options for time‐series RNA sequencing experiments in nonmodel organisms: An example from grasses

Reproductive Systems in Paspalum: Relevance for Germplasm Collection and Conservation, Breeding Techniques, and Adoption of Released Cultivars

How does drought affect native grasses’ photosynthesis on the revegetation of iron ore tailings?

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