Parylene-C Coating Protects Resin-3D-Printed Devices from Material Erosion and Prevents Cytotoxicity toward Primary Cells

Musgrove, Hannah B.; Cook, Sophie R.; Pompano, Rebecca R.

doi:10.1021/acsabm.3c00444

Cited by 3 publications

(2 citation statements)

References 27 publications

(58 reference statements)

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“…Digital light processing (DLP), a three-dimensional (3D) additive manufacturing technique, is low-cost, rapid, and high-resolution. − It enables the precise fabrication of customized devices and just-in-time prototypes. Available DLP resins, however, mostly comprise monomeric and/or oligomeric (meth)acrylates, with their leachates, be that uncured monomers or additives, causing significant cell death in vitro and in vivo . − Even commercial resins certified as biocompatible leach toxic monomers and additives, such as surfactants and plasticizers, are used to make the cured resin more flexible. , Postprinting treatments such as extensive washing, UV postcuring, heat treatment, and coating with biocompatible materials ameliorate some of the toxicity concerns. ,, Furthermore, these treatments often significantly lengthen the fabrication process, contradicting the premise of rapid prototyping. As a result, despite additive manufacturing’s many promises, the healthcare community has been slow to adapt.…”

Section: Introductionmentioning

confidence: 99%

ABA Block Copolyester Thiol–Ene Resin From Triethylborane-Assisted Ring-Opening Copolymerization

Ma,

Miller,

Wang

2024

ACS Appl. Polym. Mater.

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Leveraging metal-free ring-opening copolymerization catalyzed by a triethylborane and phosphazene base, we have developed a cytocompatible, thiol−ene-based block copolyester photoresin (BC1) with an ABA triblock architecture. A sebacatederived polyester acting as the chain transfer agent improves the scalability of ring-opening copolymerization and enables the direct, facile synthesis of functional sebacate-derived polyesters by circumventing the conventional high-temperature polycondensation that will often destroy reactive functional groups. BC1's ABA block copolymer design enables tunable elasticity, and its surface property supports the proliferation of human umbilical vein endothelial cells better than polycaprolactone, a polyester with a well-established safety record. The polymerization approach presented in this work expands the potential of sebacate-derived materials and will inspire the development of more elastic photoresins that incorporate safe, renewable, and affordable feedstocks such as sebacic acid and other long-chain aliphatic carboxylic acids.

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Section: Introductionmentioning

confidence: 99%

ABA Block Copolyester Thiol–Ene Resin From Triethylborane-Assisted Ring-Opening Copolymerization

Ma,

Miller,

Wang

2024

ACS Appl. Polym. Mater.

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“…Among these, Al 2 O 3 is one of the most studied candidates because of its low WVTR. On the other hand, polymeric parylene is an ultimate conformal coating solution for protection of devices, components, and surfaces in electronics, instrumentation, aerospace, medical and engineering industries [32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Moisture Barrier Layer of Al₂O₃-Parylene Dyad Structure for Use as Solar Cell Encapsulant

Kim

2024

Appl. Sci. Converg. Technol.

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We investigated different moisture barrier layers, including inorganic Al 2 O 3 films and six multilayer (dyad) barriers combined with an organic parylene C polymer. These dyads with varying thicknesses (i.e., 1-dyad, 2-dyad, and 3-dyad) were prepared on a polyethylene naphthalate (PEN) substrate. A single Al 2 O 3 film on a PEN with an optimal thickness of 70 nm effectively acted as a moisture barrier. The measured water vapor transmission rate (WVTR) was 9.64 × 10 −2 g/m 2 /day. Furthermore, a 2-dyad barrier, comprising an Al 2 O 3 layer (70 nm) paired with parylene (700 nm), exhibited an even lower WVTR of 2.01 × 10 −4 g/m 2 /day. We employed a 2-dyad barrier to laminate a flexible photovoltaic (PV) cell and assessed its performance under damp heat conditions of 85 ∘ C and 85 % relative humidity. After undergoing a damp heat test for 500 h, the PV module exhibited a light conversion efficiency of 18.25 %, which is only 2.8 % lower than that of the initial module. The 2-dyad barrier composed of Al 2 O 3 -parylene layers has been demonstrated to be highly effective in preventing moisture penetration. Thus, it is an excellent encapsulation layer for the fabrication of flexible solar modules.

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Three-Dimensionally Printed Agarose Micromold Supports Scaffold-Free Mouse Ex Vivo Follicle Growth, Ovulation, and Luteinization

Zaniker,

Hashim,

Gauthier

et al. 2024

Bioengineering

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Ex vivo follicle growth is an essential tool, enabling interrogation of folliculogenesis, ovulation, and luteinization. Though significant advancements have been made, existing follicle culture strategies can be technically challenging and laborious. In this study, we advanced the field through development of a custom agarose micromold, which enables scaffold-free follicle culture. We established an accessible and economical manufacturing method using 3D printing and silicone molding that generates biocompatible hydrogel molds without the risk of cytotoxicity from leachates. Each mold supports simultaneous culture of multiple multilayer secondary follicles in a single focal plane, allowing for constant timelapse monitoring and automated analysis. Mouse follicles cultured using this novel system exhibit significantly improved growth and ovulation outcomes with comparable survival, oocyte maturation, and hormone production profiles as established three-dimensional encapsulated in vitro follicle growth (eIVFG) systems. Additionally, follicles recapitulated aspects of in vivo ovulation physiology with respect to their architecture and spatial polarization, which has not been observed in eIVFG systems. This system offers simplicity, scalability, integration with morphokinetic analyses of follicle growth and ovulation, and compatibility with existing microphysiological platforms. This culture strategy has implications for fundamental follicle biology, fertility preservation strategies, reproductive toxicology, and contraceptive drug discovery.

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Parylene-C Coating Protects Resin-3D-Printed Devices from Material Erosion and Prevents Cytotoxicity toward Primary Cells

Cited by 3 publications

References 27 publications

ABA Block Copolyester Thiol–Ene Resin From Triethylborane-Assisted Ring-Opening Copolymerization

ABA Block Copolyester Thiol–Ene Resin From Triethylborane-Assisted Ring-Opening Copolymerization

Characteristics of Moisture Barrier Layer of Al₂O₃-Parylene Dyad Structure for Use as Solar Cell Encapsulant

Three-Dimensionally Printed Agarose Micromold Supports Scaffold-Free Mouse Ex Vivo Follicle Growth, Ovulation, and Luteinization

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Parylene-C Coating Protects Resin-3D-Printed Devices from Material Erosion and Prevents Cytotoxicity toward Primary Cells

Cited by 3 publications

References 27 publications

ABA Block Copolyester Thiol–Ene Resin From Triethylborane-Assisted Ring-Opening Copolymerization

ABA Block Copolyester Thiol–Ene Resin From Triethylborane-Assisted Ring-Opening Copolymerization

Characteristics of Moisture Barrier Layer of Al2O3-Parylene Dyad Structure for Use as Solar Cell Encapsulant

Three-Dimensionally Printed Agarose Micromold Supports Scaffold-Free Mouse Ex Vivo Follicle Growth, Ovulation, and Luteinization

Contact Info

Product

Resources

About

Characteristics of Moisture Barrier Layer of Al₂O₃-Parylene Dyad Structure for Use as Solar Cell Encapsulant