Parvovirus Transcription

Carter, Barrie J.; Laughlin, Catherine A.; Marcus-Sekura, Carol J.

doi:10.1007/978-1-4684-8012-2_5

Cited by 31 publications

(51 citation statements)

References 152 publications

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“…1. The AAV2 genome contains three promoters, P5, plg, and P40, which each yield two transcripts differing in the presence or absence of the intron (11,21,39,40,42). All of the transcripts terminate at the same 3' polyadenylation site (39,57).…”

Section: Resultsmentioning

confidence: 99%

“…When molecular clones containing the entire AAV type 2 (AAV2) genome in bacterial plasmids are transfected into mammalian cells in the presence of adenovirus, the AAV genome is excised from the plasmid and replicated to produce infectious AAV particles (38,50,52). This facilitated both the genetic analysis of AAV (27,(51)(52)(53)59) and the development of AAV as a eucaryotic expression vector (26,60,61).The AAV genome contains three transcription promoters, P5, Pi9, and P40, which yield overlapping transcripts (11). Genetic analysis showed that a major AAV reading frame (orf-2) which is accessible from P40 transcripts codes for a major portion of the AAV capsid proteins (27,59).…”

mentioning

confidence: 99%

“…The AAV genome contains three transcription promoters, P5, Pi9, and P40, which yield overlapping transcripts (11). Genetic analysis showed that a major AAV reading frame (orf-2) which is accessible from P40 transcripts codes for a major portion of the AAV capsid proteins (27,59).…”

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confidence: 99%

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Negative and positive regulation in trans of gene expression from adeno-associated virus vectors in mammalian cells by a viral rep gene product.

Tratschin¹,

Tal²,

Carter³

1986

Mol. Cell. Biol.

149

143

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We previously described use of the human parvovirus, adeno-associated virus (AAV), as a vector for transient expression in mammalian cells of the gene for chloramphenicol acetyltransferase (CAT). In the AAV vector, pTS1, the CAT gene is expressed under the control of the major AAV promoter p4o. This The human parvovirus, adeno-associated virus (AAV), replicates in mammalian cells in the presence of helper functions provided by adenovirus or herpesvirus (10). When molecular clones containing the entire AAV type 2 (AAV2) genome in bacterial plasmids are transfected into mammalian cells in the presence of adenovirus, the AAV genome is excised from the plasmid and replicated to produce infectious AAV particles (38,50,52). This facilitated both the genetic analysis of AAV (27,(51)(52)(53)59) and the development of AAV as a eucaryotic expression vector (26,60,61).The AAV genome contains three transcription promoters, P5, Pi9, and P40, which yield overlapping transcripts (11). Genetic analysis showed that a major AAV reading frame (orf-2) which is accessible from P40 transcripts codes for a major portion of the AAV capsid proteins (27,59). Mutations in this region (cap-) allow normal synthesis of duplex replicating form DNA but prevent synthesis of progeny single-stranded DNA and infectious particles (27,53,59). A second major open reading frame (orf-1) in the left half of the genome is apparently accessible from either p5 or P19 transcripts and codes for one or more proteins (rep) required for AAV DNA replication (27,53,59). Mutations in this region (rep-) are DNA negative but can be complemented. Deletion of both terminal palindromes results in a cis-dominant (ori-) defect which reflects the presence of the AAV replication origins in the terminal repeat sequences (3,4,25,51,53).

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Negative and positive regulation in trans of gene expression from adeno-associated virus vectors in mammalian cells by a viral rep gene product.

Tratschin¹,

Tal²,

Carter³

1986

Mol. Cell. Biol.

149

143

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“…which indicates the location of the three transcription promoters (p5. Pi9, and P40), the 335-nucleotide intron, and the polyadenylation site (3,7,16,27,28,32,44). The AAV vectors described here were constructed to allow expression of the inserted tneo gene under the control of the AAV P40 transcription unit.…”

Section: Methodsmentioning

confidence: 99%

Adeno-associated virus vector for high-frequency integration, expression, and rescue of genes in mammalian cells.

Tratschin¹,

Miller²,

Smith³

et al. 1985

Mol. Cell. Biol.

144

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“…These (10)(11)(12). When no helper virus is available, AAV can persist as an integrated provirus (2, 13-16).…”

Section: Introductionmentioning

confidence: 99%

Use of adeno-associated virus as a mammalian DNA cloning vector: transduction of neomycin resistance into mammalian tissue culture cells.

Hermonat¹,

Muzyczka²

1984

Proc. Natl. Acad. Sci. U.S.A.

407

234

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Adeno-associated virus (AAV) is a human DNA virus that has a broad host range and can be grown both as an integrated provirus and as a lytic genome. These (10)(11)(12). When no helper virus is available, AAV can persist as an integrated provirus (2, 13-16). Integration apparently involves recombination between AAV termini and host sequences and most of the AAV sequences remain intact (15,16) in the provirus. The ability of AAV to integrate into host DNA is apparently a strategy for insuring the survival of AAV sequences in the absence of the helper virus (16). When cells carrying an AAV provirus are subsequently superinfected with a helper, the integrated AAV genome is rescued and a productive lytic cycle occurs (2).Much of the recent genetic work with AAV (17-19) has been facilitated by the discovery that AAV sequences that have been cloned into prokaryotic plasmids are infectious (17). When the wild-type (wt) AAV/pBR322 plasmid pSM620 is transfected into human cells in the presence of adenovirus, the AAV sequences are rescued from the plasmid and a normal AAV lytic cycle ensues (17). This means that it is possible to modify the AAV sequences in the recombinant plasmid and then to grow a viral stock of the mutant by transfecting the plasmid into human cells (18,19).Using this approach, we have demonstrated the existence of at least three phenotypically distinct regions (Fig. 1) in AAV (19). The rep region codes for one or more proteins that are required for DNA replication and for rescue from the recomThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact. binant plasmid. The cap and lip regions (Fig. 1) appear to code for AAV capsid proteins and mutants within these regions are capable of DNA replication (19). In addition, studies of terminal mutants show that the AAV termini are required for DNA replication (18).In this study, we isolate a recombinant AAV viral stock that carries a dominant selectable marker. Using this stock, we demonstrate that AAV can be used to introduce foreign DNA into mammalian cells. Furthermore, we show that when the transduced cells are superinfected with adenovirus, the integrated recombinant genome can be rescued and amplified. These experiments suggest that AAV may be useful as a generalized transduction vector with the unique property that the transduced sequences can be easily recovered from infected cells. MATERIALS AND METHODS

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Parvovirus Transcription

Cited by 31 publications

References 152 publications

Negative and positive regulation in trans of gene expression from adeno-associated virus vectors in mammalian cells by a viral rep gene product.

Negative and positive regulation in trans of gene expression from adeno-associated virus vectors in mammalian cells by a viral rep gene product.

Adeno-associated virus vector for high-frequency integration, expression, and rescue of genes in mammalian cells.

Use of adeno-associated virus as a mammalian DNA cloning vector: transduction of neomycin resistance into mammalian tissue culture cells.

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