Parvovirus: the expanding spectrum of disease

Heegaard, Erik D.; Hornsleth, A

doi:10.1111/j.1651-2227.1995.tb13591.x

Cited by 70 publications

(35 citation statements)

References 69 publications

(115 reference statements)

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“…However, immunocompromised patients (such as patients with HIV or patients with cancer who are undergoing chemotherapy), pregnant women, and, increasingly, transplant recipients are at significant risk for B19 infection [1,2]. Many such individuals are unable to produce neutralizing antibodies to clear the virus, and this can lead to a persistent infection resulting in anemia [1].…”

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confidence: 99%

B Cell Memory Is Directed toward Conformational Epitopes of Parvovirus B19 Capsid Proteins and the Unique Region of VP1

Corcoran¹,

Mahon²,

Doyle³

2004

J INFECT DIS

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Background. Loss of antibody reactivity against linear epitopes of parvovirus B19 (B19) capsid proteins VP1 and VP2 occurs after infection; however, it is unclear whether B cell memory is established against linear epitopes.Methods. B cell enzyme-linked immunospot assay was used to evaluate B19-specific B cell memory in volunteer donors ( ). n p 22 Results. B cell memory is maintained against conformational epitopes of VP2 and is absent against linear epitopes of VP2. Individuals seronegative for IgG against the unique region of VP1 have detectable B cell memory, with the potential to mount a humoral response on reexposure to B19. Conversely, in mice immunized with VP2, long-lasting IgG against linear epitopes of VP2 and a strong B cell-memory response are observed.Conclusions. B cell memory is established and maintained against conformational epitopes of VP2 and against linear epitopes of VP1 but not against linear epitopes of VP2. These findings further our understanding of the immune response to B19 and suggest that analysis of B19-specific B cell memory merits consideration for future B19-vaccine studies.

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confidence: 99%

B Cell Memory Is Directed toward Conformational Epitopes of Parvovirus B19 Capsid Proteins and the Unique Region of VP1

Corcoran¹,

Mahon²,

Doyle³

2004

J INFECT DIS

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“…As mentioned above and elsewhere, increasing numbers of cases involving B19 infection among patients suffering from haematological disorders have been reported [13,14]. A retrospective study by Frickhofen et al has found evidence of B19 infection in 8/57 (14%) patients with acquired chronic pure red cell aplasia by demonstrating viral DNA in their sera [15].…”

Section: Discussionmentioning

confidence: 99%

“…The study by Heegaard et al [14] showed that 12 of 13 B19 positive patients presented associated conditions (which were mentioned above). Similarly, all of our patients were diagnosed as suffering from a variety of specific diseases and B19 was thought to represent a coincidental complication.…”

Section: Discussionmentioning

confidence: 99%

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The investigation of parvovirus B19 infection in patients with haematological disorders by using PCR and ELISA techniques

Us¹,

Ozune²,

Kaşifoğlu³

et al. 2007

Braz J Infect Dis

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Parvovirus B19 has a marked tropism for erythroid progenitor cells. This may lead to chronic anemia in predisposed individuals. The purpose of the study was to investigate the frequency of parvovirus B19 infections in patients with diagnosis of haematological disorders. In order to determine the diagnostic use of different markers of parvovirus B19 infection, serum specimens obtained from 79 patients with haematological disorders were tested for specific antibodies and viral DNA through the use of ELISA and PCR techniques. Evidence of parvovirus B19 infection was found in 23/79 (29.1%) patients by demonstrating viral DNA and/or specific IgM antibody. B19 infection was established in 3 of 11 patients with chronic myeloid leukemia, in 3 of 11 acute myeloid leukemia, in 2 of 11 patients with multiple myeloma, in 3 of 8 patients with Hodgkin's lymphoma, in 5 of 10 patients with non-Hodgkin's lymphoma, in 1 of 6 patients with myelodysplastic syndrome, in 4 of 11 patients with chronic lymphocytic leukemia, and in 2 of 11 patients with acute lymphocytic leukemia. In 4 of 23 positive patients, only parvovirus B19 DNA could be detected, while 7 patients were tested positive for both parvovirus B19 DNA and specific IgM. Nine patients were tested positive for both B19 DNA and specific IgG. In the remaining 3 positive patients only specific IgM could be detected. Due to the discrepancies between DNA and IgM results, the diagnostic procedures should include a search for specific DNA by PCR methods if specific IgM has been found to be negative.

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New LightCycler PCR for Rapid and Sensitive Quantification of Parvovirus B19 DNA Guides Therapeutic Decision-Making in Relapsing Infections

Harder¹,

Hufnagel

Zahn³

et al. 2001

J Clin Microbiol

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The human erythrovirus B19, a member of the family Parvoviridae, causes a broad and seemingly expanding spectrum of disorders (7,12,23,44). The clinical picture depends on the immune status and age of the patient (14,36,43). Parvovirus B19 shows a remarkable tropism for erythroid progenitor cells in the bone marrow, which is partly based on binding to its receptor, the blood group P antigen (6). Viral replication, and possibly also the induced immune response, interferes with physiological functions and loss of the erythroid progenitor cells, resulting in a usually subclinical reticulocytopenia (8). In patients with an underlying hematologic disorder and high blood cell turnover (e.g., hemolytic anemia), transient aplastic anemia may ensue. Immunocompromised patients, who have an increased risk of developing a persistent B19 infection, are threatened by a chronic reticulocytopenic anemia, also known as pure red cell anemia (2,8).Diagnosis of uncomplicated cases of acute B19 infection (fifth disease or arthropathy) is usually clinically based and can be accomplished by detection of specific immunoglobulin M (IgM) antibodies except in immunocompromised patients, who are prone to persistent infection and who may generate IgMspecific B19 antibodies less reliably (7,19). Likewise, specific IgG is not a reliable marker for discriminating a reconvalescent status from chronic persistent infection (35), although recent data indicate that IgG antibodies specific for nonstructural protein 1 (NS-1) of B19 are more frequently associated with persistent infection (24). Detection of DNA by hybridization or PCR has been reported to be superior in the diagnosis of prenatal B19 infections and in children with oncologic or hematologic disorders (5,11,34). Several qualitative and quantitative PCR methods targeting different regions of the parvovirus genome have been published (1,4,9,10,13,15,17,22,25,40). These PCR approaches are based on conventional block thermocycling and combined single-round PCRs with subsequent oligohybridization or use a nested format.The recently developed LightCycler (LC) DNA amplification technology (Roche Diagnostics, Mannheim, Germany) combines rapid glass capillary thermal cycling with real-time microvolume fluorescence monitoring (47). Detection of amplicons is achieved during the run in real time. Melting point analysis of the amplicons at the end of the run is used as a specificity control when the fluorochrome SYBR green is used for detection of double-stranded DNA. Alternatively, specificity can be tested during the run by using two target-specific hybridization probes which utilize fluorescence resonance energy transfer (FRET) to generate a measurable signal. In the latter case, two oligonucleotide probes (HybProbe) bind to immediately adjacent regions of the respective amplicon. The upstream probe (referred to as the probe) is labeled at its 3Ј end with fluorescein, while the 5Ј end of the downstream probe (referred to as the anchor) is labeled with either of the fluorochromes LC-Red 640 and LC-...

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Parvovirus: the expanding spectrum of disease

Cited by 70 publications

References 69 publications

B Cell Memory Is Directed toward Conformational Epitopes of Parvovirus B19 Capsid Proteins and the Unique Region of VP1

B Cell Memory Is Directed toward Conformational Epitopes of Parvovirus B19 Capsid Proteins and the Unique Region of VP1

The investigation of parvovirus B19 infection in patients with haematological disorders by using PCR and ELISA techniques

New LightCycler PCR for Rapid and Sensitive Quantification of Parvovirus B19 DNA Guides Therapeutic Decision-Making in Relapsing Infections

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