Parvalbumin correlates with relaxation rate in the swimming muscle of sheepshead and kingfish

Abstract

expression of parvalbumin isoforms will differ between the anterior and posterior red muscle, but little longitudinal variation will be observed in parvalbumin expression in white and pink muscle. We successfully employed protein electrophoresis (SDS-PAGE) with western blots to identify two parvalbumin isoforms in each muscle fiber type. SDS-PAGE and densitometry were used to determine the relative expression levels of the two parvalbumin isoforms and total parvalbumin expression. Red muscle displays a significant shift, from anterior to posterior, in the relative expression of the two isoforms, both in their relative contribution and in total parvalbumin content, but white and pink muscle did not. The red muscle of southern kingfish, Menticirrhus americanus (Pisces, F. Scianidae) showed a pattern similar to the red muscle of sheepshead.

Differential expression of genes and proteins between electric organ and skeletal muscle in the mormyrid electric fishBrienomyrus brachyistius

“…This finding is consistent with studies by Childers and Siegel, which examined parvalbumin concentrations in SM and EO of E. electricus (Childers and Siegel, 1976). Parvalbumins are considered crucial in excitation-contraction coupling in muscle (Arif, 2009;Wilwert et al, 2006). Typically, muscles with fast relaxation rates express higher levels of parvalbumins than more powerful, slow-contracting muscles (Wilwert et al, 2006).…”

“…Parvalbumins are considered crucial in excitation-contraction coupling in muscle (Arif, 2009;Wilwert et al, 2006). Typically, muscles with fast relaxation rates express higher levels of parvalbumins than more powerful, slow-contracting muscles (Wilwert et al, 2006). Thus, the decreased expression of parvalbumins in EO is consistent with the fact that EOs is noncontractile.…”

Regional variation in parvalbumin isoform expression correlates with muscle performance in common carp (Cyprinus carpio)

“…Our study shows that the anterior skeletal muscle of carp does have significantly more PARV than posterior muscle by a similar magnitude to that reported by Thys and colleagues for fast-twitch muscle from widemouthed bass (Thys et al, 2001). Earlier studies have also found regional differences in PARV isoform composition in fast-twitch muscle in some fish species: brook trout (Coughlin et al, 2007), barbel (Huriaux et al, 1997), largemouth bass (Thys et al, 2001), sheepshead and kingfish (Wilwert et al, 2006). Our study expands on these earlier studies by examining a complete set of PARV isoforms, in turn, revealing a more complicated pattern of changes including increasing and decreasing expression with significantly higher proportions of β4 and β5 and significantly lower amounts of β1 in anterior compared with posterior muscle.…”

Differences in locomotor performance between individuals: importance of parvalbumin, calcium handling and metabolism

“…We provide here the first experimental evidence that locomotor performance is also influenced by parvalbumin concentration. At the level of isolated muscles, parvalbumin expression is positively associated with relaxation time (Münterer et al, 1995;Brownridge et al, 2009;Schoenman et al, 2010), and differences in relaxation time of different muscles within the same organism are correlated with differences in parvalbumin content (Wilwert et al, 2006;Brownridge et al, 2009). There may also be functional differences between different parvalbumin isoforms (Brownridge et al, 2009;Schoenman et al, 2010).…”