Parvalbumin and calbindin-D28K immunoreactivity as developmental markers of auditory and vocal motor nuclei of the zebra finch

Braun, Katharina; Scheich, Henning; Heizmann, Claus W.; Hunziker, Willi

doi:10.1016/0306-4522(91)90017-i

Cited by 78 publications

(59 citation statements)

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“…Conversely to calbindin, parvalbumin is a useful marker of neurons with high levels of electrical and metabolic activity (Braun et al, 1985(Braun et al, , 1991Kawaguchi et al, 1987). This protein labels efferent neurons whose axons exit the SC via the tectoreticular pathway en route to the contralateral brainstem (Meredith and Stein, 1986;Wallace et al, 1993;McHaffie et al, 2001;Fuentes-Santamaria et al, 2006), which is consistent with the present study reporting parvalbumin within intermediate and large multipolar neurons in the deep layers of the SC in control and epileptic animals.…”

Section: Figuresupporting

confidence: 91%

“…Calcium-binding proteins contribute to regulation of intracellular calcium concentration in neurons in the nervous system (Baimbridge et al, 1992). Interestingly, it has been suggested that intracellular calcium, and therefore, calcium-binding protein levels, could be regulated by modifications in neuronal electrical activity (Braun et al, 1991;Winsky and Jacobowitz, 1995; Fuentes-Santamaria et al, 2003, 2005bAlvarado et al, 2004Alvarado et al, , 2005. One of these proteins, calbindin, has been used in the present study as a neuronal marker for investigating possible activity-related changes in the superficial layers of the SC of the GPG/Vall hamster.…”

Section: Figurementioning

confidence: 99%

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Morphologic and neurochemical alterations in the superior colliculus of the genetically epilepsy-prone hamster (GPG/Vall)

et al. 2007

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SummaryThe GPG/Vall hamster is an animal model that exhibits seizures in response to sound stimulation. Since the superior colliculus (SC) is implicated in the neuronal network of audiogenic seizures (AGS) in other forms of AGS, this study evaluated seizure-related anatomical or neurochemical abnormalities in the SC of the GPG/Vall hamster. This involved calbindin (CB) and parvalbumin (PV) immunohistochemistry, densitometric analysis and high performance liquid chromatography in the superficial and deep layers of the SC in control and epileptic animals. Compared to control animals, a reduction in SC volume and a hypertrophy of neurons located in the deep layers of the SC were observed in the epileptic hamster. Although, analysis of CB-immunohistochemistry in the superficial layers did not show differences between groups, analysis of PV-immunostaining in the deep SC revealed an increase in the mean gray level within immunostained neurons as well as a decreased immunostained neuropil in the GPG/Vall hamster as compared to control animals. These alterations were accompanied by a decrease in the levels of GABA and increased levels of taurine in the epileptic animal. These data indicate that the deep SC of the GPG/Vall hamster is structurally abnormal; suggesting its involvement in the neuronal network for AGS.

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Section: Figuresupporting

confidence: 91%

Section: Figurementioning

confidence: 99%

Morphologic and neurochemical alterations in the superior colliculus of the genetically epilepsy-prone hamster (GPG/Vall)

et al. 2007

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“…PV-expression in the songbird is confined to specific neuronal populations that are especially involved in song-learning [80][81][82]. This finding suggests that PV fulfills a specific function in these neurons that is potentially related to their morphological plasticity in the context of vocal learning (see also [83]); or, alternatively, to their protection against Ca 2+ -toxicity from the highly active neurons that control some of the fastest muscles in the body [84].…”

Section: Discussionmentioning

confidence: 97%

Reduction in 50-kHz call-numbers and suppression of tickling-associated positive affective behaviour after lesioning of the lateral hypothalamic parvafox nucleus in rats

Roccaro-Waldmeyer

Babalian

Müller

et al. 2016

Behavioural Brain Research

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h i g h l i g h t s• Wistar rats were tickled before and after excitotoxic lesioning of the lateral hypothalamic parvafox nucleus.• Ultrasonic vocalisations (USVs) that were emitted during the tickling of rats and their tendency to approach and follow the experimenter's hand were analysed.• Lesioning was considered successful if the number of parvalbuminimmunoreactive (PV-ir) cells in the area of the parvafox nucleus was reduced beyond a threshold level.• Rats with bilaterally successful lesions manifested the most profound surgery-associated reduction in the number of 50-kHz USVs and in the tendency to approach and follow the experimenter's hand.• Positive correlations were found between each of the four investigated parameters. g r a p h i c a l a b s t r a c tThe parvafox nucleus is located ventrolaterally in the lateral hypothalamic area (LHA). Its core and shell are composed of neurons expressing the calcium-binding protein parvalbumin (PV) and the transcription factor Foxb1, respectively. Given the known functions of the LHA and that the parvafox nucleus receives afferents from the lateral orbitofrontal cortex and projects to the periaqueductal gray matter, a functional role of this entity in the expression of positive emotions has been postulated.The purpose of the present study was to ascertain whether the deletion of neurons in the parvafox nucleus influenced the tickling-induced 50-kHz calls, which are thought to reflect positive affective states, in rats. To this end, tickling of the animals (heterospecific play) was combined with intracerebral injections of the excitotoxin kainic acid into the parvafox nucleus.The most pronounced surgery-associated reduction in 50-kHz call-numbers was observed in the group of rats in which, on the basis of PV-immunoreactive-cell counts in the parvafox nucleus, bilateral lesions had been successfully produced. Two other parameters that were implemented to quantify positive affective behaviour, namely, an approach towards and a following of the hand of the tickling * Corresponding author at: Institute of Anatomy, University of Fribourg, Rte A. Gockel 1, CH-1700 Fribourg, Switzerland.E-mail address: marco.celio@unifr.ch (M.R. Celio). 1Published in which should be cited to refer to this work.http://doc.rero.ch experimenter, were likewise most markedly suppressed in the group of rats with bilaterally successful lesions. Furthermore, positive correlations were found between each of the investigated parameters. Our data afford evidence that the parvafox nucleus plays a role in the production of 50-kHz calls in rats, and, more generally, in the expression of positive emotions.

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“…While calretinin was the only CaBP found in the chick auditory brain stem nuclei (Parks et al 1997;Rogers 1989a), three members of the CaBPs family, calretinin, parvalbumin, and calbindin, have been found in the mammalian auditory system (Bazwinsky et al 2005(Bazwinsky et al , 2008Braun et al 1991b;Braun and Piepenstock 1993;Caicedo et al 1996Caicedo et al , 1997Celio 1990;Förster and Illing 2000;Fredrich et al 2009;Friauf 1993Friauf , 1994Idrizbegovic et al 2001Idrizbegovic et al , 2004Idrizbegovic et al , 2006Kelley et al 1992;Kulesza 2014;Logerot et al 2011;Lohmann and Friauf 1996;Matsubara 1990;Vater and Braun 1994). These CaBPs formed complementary patterns of expression in the cochlear nuclei (Bazwinsky et al 2008;Fredrich et al 2009;Lohmann and Friauf 1996).…”

Section: Comparison With Mammalian Auditory Brain Stem Expressionmentioning

confidence: 99%

“…The auditory brain stems of birds and mammals have been characterized by high expression levels of three calcium-binding proteins (CaBP): calretinin, calbindin, and parvalbumin (Braun 1990;Braun et al 1985Braun et al , 1991bCelio and Heizmann 1981;Friauf 1994;Kubke et al 1999;Lohmann and Friauf 1996;Parks et al 1997). The calcium buffering capability of these CaBPs may play a neuroprotective role against high activity levels or degeneration during hearing loss (Camp and Wijesinghe 2009;D'Orlando et al 2002;Idrizbegovic et al 2006;Parks et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Bloom

Williams

MacLeod

2014

JARO

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Multiple calcium-binding proteins (CaBPs) are expressed at high levels and in complementary patterns in the auditory pathways of birds, mammals, and other vertebrates, but whether specific members of the CaBP family can be used to identify neuronal subpopulations is unclear. We used double immunofluorescence labeling of calretinin (CR) in combination with neuronal markers to investigate the distribution of CR-expressing neurons in brainstem sections of the cochlear nucleus in the chicken (Gallus gallus domesticus). While CR was homogeneously expressed in cochlear nucleus magnocellularis, CR expression was highly heterogeneous in cochlear nucleus angularis (NA), a nucleus with diverse cell types analogous in function to neurons in the mammalian ventral cochlear nucleus. To quantify the distribution of CR in the total NA cell population, we used antibodies against neuronal nuclear protein (NeuN), a postmitotic neuronspecific nuclear marker. In NA neurons, NeuN label was variably localized to the cell nucleus and the cytoplasm, and the intensity of NeuN immunoreactivity was inversely correlated with the intensity of CR immunoreactivity. The percentage of CR+neurons in NA increased from 31 % in embryonic (E)17/18 chicks, to 44 % around hatching (E21), to 51 % in postnatal day (P) 8 chicks. By P8, the distribution of CR + neurons was uniform, both rostrocaudal and in the tonotopic (dorsoventral) axis.Immunoreactivity for the voltage-gated potassium ion channel Kv1.1, used as a marker for physiological type, showed broad and heterogeneous postsynaptic expression in NA, but did not correlate with CR expression. These results suggest that CR may define a subpopulation of neurons within nucleus angularis.

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Parvalbumin and calbindin-D28K immunoreactivity as developmental markers of auditory and vocal motor nuclei of the zebra finch

Cited by 78 publications

References 40 publications

Morphologic and neurochemical alterations in the superior colliculus of the genetically epilepsy-prone hamster (GPG/Vall)

Morphologic and neurochemical alterations in the superior colliculus of the genetically epilepsy-prone hamster (GPG/Vall)

Reduction in 50-kHz call-numbers and suppression of tickling-associated positive affective behaviour after lesioning of the lateral hypothalamic parvafox nucleus in rats

Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

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