Parvalbumin, a cross-reactive fish allergen, contains IgE-binding epitopes sensitive to periodate treatment and Ca2+ depletion

Recombinant Carp Parvalbumin, the Major Cross-Reactive Fish Allergen: A Tool for Diagnosis and Therapy of Fish Allergy

“…In this context it was found that several calcium-binding allergens exhibited varying IgE binding capacities depending on the presence or the absence of proteinbound calcium (24,43). To test the influence of calcium on the IgE binding of recombinant carp parvalbumin, we exposed sera from six representative fish-allergic patients to nitrocellulose-blotted rCyp c 1.01 in the presence (ϩ lanes ) or the absence (Ϫ lanes ) of protein-bound calcium (Fig.…”

“…It was further shown that patients who mount IgE Abs against one parvalbumin will cross-react with the homologous proteins from other fish species (24), which demonstrates the importance of parvalbumins as cross-reactive fish allergens and explains why allergic individuals exhibit clinical symptoms upon contact with various fish species. IgE competition experiments performed with purified carp parvalbumin indicated that this molecule contained a large portion of IgE epitopes present in various fish species (25).…”

“…Resistance to boiling and to enzymes of the gastrointestinal tract may, in fact, be a predisposing factor that these proteins can act as potent sensitizing agents for Ͼ95% of fish allergic patients (6,(21)(22)(23)(24). It was further shown that patients who mount IgE Abs against one parvalbumin will cross-react with the homologous proteins from other fish species (24), which demonstrates the importance of parvalbumins as cross-reactive fish allergens and explains why allergic individuals exhibit clinical symptoms upon contact with various fish species.…”

Hypoallergenic molecules for subcutaneous immunotherapy

“…Carp parvalbumin (Cyp c 1) contains most cross-reactive IgE epitopes present in the family of homologous fish parvalbumin. [23] In 2007, a mutant (m) Cyp c 1, [24] was created with the two functional Ca 2+ -binding sites mutated, rendering the molecule hypoallergenic. As in many similar papers, the tentative conclusion: "this molecule might be an interesting candidate for future immunotherapy" was proposed.…”

Development of a real-time PCR system for the detection of the potential allergen fish in food

“…The consumption of fish by affected persons may cause severe anaphylactic shocks with probably fatal consequences. Currently, about 0.1 % of the population suffers from fish allergy and because of the increasing consumption of fishery products, the prevalence is rising [3]. Even small amounts of a few milligrams of protein can lead to allergic reactions immediately [4].…”