Parts From Life-Cycle of H.ERINACEUS: Response Surface Methodology Approach to Optimize Extraction Conditions and Determination of Its Antioxidant, Antidiabetic and Antimicrobial Effect

Abstract

In this article, optimization of extraction conditions of different parts from the life cycle of H.erinaceus was investigated based on Response Surface Method. Furthermore, antioxidant, antidiabetic, and antimicrobial effects of optimized extracts were studied. Results: Temperature, liquid / solid ratio, and solvent concentration significantly affected the phenolic content and antioxidant capacities of all samples. The young fruit body of H.erinaceus had higher antioxidant (43.11±2.74, 51.39±2.34, 62.84±1.59 µmol TE/g for DPPH, FRAP and ABTS, respectively) and antidiabetic effects (93.54±2.3 and 132.25±1.38 µg/mL for IC50 values of α-glucosidase and α-amylase, respectively) than mycelium. However, these effects partially decrease as the fruit body ages. Besides, the optimized extracts exhibited more antimicrobial effects on Gram-positive bacteria than Gram-negative bacteria. Conclusion: Both the fruit body and the mycelium of H.erinaceus have many bio-functional properties. However, these properties of the fruit body decrease significantly with the delay of the harvest time. The extraction process conditions we optimize in this study can be adapted to large-scale separation processes in the industry.

Using Phenol-Rich Agro-Wastes as Substrates for the Cultivation of Hypsizygus ulmarius Mushroom with Enhanced Functional and Nutritional Potential

“…Moreover, the present work assessed for the first time the TPC of H. ulmarius cultivated on different growing media supplemented with phenolic-rich agro-wastes. The TPC of H. ulmarius fruitbodies grown on different media varied between 2.65 and 4.26 mg GAE g -1 compared to the reported values in other mushroom species including Lentinula edodes (18.03 mg GAE g -1 ) [50], Ganoderma lucidum (2.107 mg GAE g -1 ), Pleurotus ostreatus (36.0 mg GAE g -1 ) [51], Hericium erinaceus mycelium and fruitbodies (3.82-4.89 mg GAE g -1 ) [52]. Generally, it seemed that increases in TPC concentration were found to be significantly associated with higher ratios of GP and GWH in the growing media.…”

Güve-Alev Optimizasyon Algoritması Kullanarak Pleurotus cornucopiae var. citrinopileatus Mantarı Ekstraksiyon Koşullarının Optimizasyonu

“…Biyoaktif bileşiklerin ekstraksiyonu için en yaygın olarak kullanılan solventler etanol, metanol, aseton gibi çözücüler veya bunların su ile karışımının kullanıldığı çözeltilerdir [19]. Ayrıca bu bileşiklerin ekstraksiyonunun, ekstraksiyon sıcaklığı, zamanı, solvent türü ve oranı, ekstraksiyon konsantrasyonu, solvent-katı oranı, partikül boyutu ve pH gibi farklı ekstraksiyon koşullarından etkilendiği yapılan birçok çalışma ile bildirilmiştir [20], [24]. Dolayısıyla ekstraksiyon koşullarının optimize edilmesi, hedef bileşiklerden en verimli şekilde yararlanmak için çok önemli bir adımdır.…”