Partitioning of Recombinant Pseudomonas putida POS-F84 Proline Dehydrogenase in Aqueous Two-Phase Systems: Optimization Using Response Surface Methodology

Omidinia, Eskandar; Shahbazmohammadi, Hamid; MohseniPour, Zeinab; Mahdizadeh, Rahman

doi:10.1007/s12010-019-03011-3

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…Among different statistical tools used in scientific research response surface methodology (RSM) is one of the popular statistical tools extensively used for optimizing the cultural conditions during fermentation process of various enzymes in recent years. This statistical approach has been used by many researchers for optimizing the production of enzymes from microorganisms [ 38 , 39 , 40 , 41 ]. The economics of the fermentation process for the production of enzymes in industries is one of the most crucial factors.…”

Section: Resultsmentioning

confidence: 99%

Response surface methodology: An effective optimization strategy for enhanced production of nitrile hydratase (NHase) by Rhodococcus rhodochrous (RS-6)

Sahu

Meghavarnam

Janakiraman

2020

Heliyon

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Nitrile hydratase is an enzyme which catalyze the hydration of nitriles into amide and their role as catalysts for acrylamide production in industries are well known. The present study aims at statistically optimizing physiological and nutritional parameters for NHase production from Rhodococcus rhodochrous (RS-6). The effect of incubation period, temperature, pH, carbon and nitrogen sources on the production of NHase was investigated by one factor at a time strategy. Further optimization process was carried out by response surface methodology for studying the interactive effect of these variables using central composite design. The optimized levels of variables obtained by statistical analysis were: incubation period 48 h, temperature 33 °C, pH 7.0, glycerol 1% and urea 0.75%, which resulted in maximum NHase production. The results of ANOVA were significant with the F -value of the model being 296.78, value of R 2 is 0.9983 and the lack of fit test was not significant. The contour and response surface plots showed significant interaction between the variables. The NHase yield was enhanced up to 6.22 fold by statistical optimization using RSM. Thus, the developed experimental design is effective towards process optimization for NHase production from R . rhodochrous (RS-6).

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Section: Resultsmentioning

confidence: 99%

Response surface methodology: An effective optimization strategy for enhanced production of nitrile hydratase (NHase) by Rhodococcus rhodochrous (RS-6)

Sahu

Meghavarnam

Janakiraman

2020

Heliyon

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“…Albertsson later used these systems for separating biological compounds [2,14]. This discovery has provided the way for separating different kinds of biomolecules such as proteins [15,16], enzymes [17,18], amino acids [19,20], antioxidants [21][22][23], antibiotics [24][25][26], and DNA [27]. Typically, ATPSs are formed by the aqueous solution of two incompatible polymers, one salt with one polymer, or two salts, resulting in two immiscible phases.…”

Section: Introductionmentioning

confidence: 99%

Examining the Phase Equilibria of Choline Chloride + Tripotassium Phosphate + Water and Exploiting the Liquid- Liquid Zone for Biomolecule Partitioning: Influence of Salt Additives

Norouzi

2024

Preprint

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Initially, the phase diagram for tripotassium phosphate + choline chloride + water was established by delineating binodals encompassing the solid-liquid-liquid triphasic zone, solid-liquid biphasic zone, liquid-liquid biphasic zone, and the liquid monophasic zone. Tie-lines within the liquid-liquid biphasic zone, serving as an aqueous two-phase system (ATPS), were determined through equilibrium concentration measurements of the components. The ATPS was applied for the partitioning of bovine serum albumin (BSA) and L-tryptophan. Results indicated that the current ATPS is unsuitable for BSA partitioning due to its low solubility, whereas it exhibited notable efficacy in L-tryptophan partitioning, achieving a maximum partition coefficient of 150.65. Subsequently, the impact of two salt additives, NaCl and K2CO3, on enhancing L-tryptophan partitioning in the ATPS was explored. Both salts increased the partition coefficient, with K2CO3 demonstrating significantly greater effectiveness.

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Partitioning of Recombinant Pseudomonas putida POS-F84 Proline Dehydrogenase in Aqueous Two-Phase Systems: Optimization Using Response Surface Methodology

Cited by 2 publications

References 18 publications

Response surface methodology: An effective optimization strategy for enhanced production of nitrile hydratase (NHase) by Rhodococcus rhodochrous (RS-6)

Response surface methodology: An effective optimization strategy for enhanced production of nitrile hydratase (NHase) by Rhodococcus rhodochrous (RS-6)

Examining the Phase Equilibria of Choline Chloride + Tripotassium Phosphate + Water and Exploiting the Liquid- Liquid Zone for Biomolecule Partitioning: Influence of Salt Additives

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