Partitioning of hydrophobic amino acids and oligopeptides in aqueous two-phase system containing self-aggregating block copolymer effects of temperature, salts and surfactants

Phase equilibrium in aqueous two-phase systems containing ethylene oxide–propylene oxide block copolymers and dextran

“…Moreover, there is a remarkable change in the phase diagrams when temperature is raised from 5 to 40 • C, which was ascribed to changes on the aggregation state of the copolymer molecules. Further work by the same group was reported by Svensson et al [12], who investigated the partitioning behavior of aminoacids, dipeptides and tripeptides in ATPS formed by dextran T500 and the block copolymer P105, and by Svensson et al [13], who determined the partition coefficients of some proteins in ATPS formed by dextran T500 and either F68 or P105.…”

Quaternary (liquid+liquid) equilibria of aqueous two-phase poly (ethylene glycol), poly (DMAM–TBAM), and KH2PO4: Experimental and generalized Flory–Huggins theory

“…On comparing figures 2 and 3, it becomes clear that the addition of copolymer similar to temperature increase can expand the two-phase area of ATPS. The binodal curves of the second type of ternary ATPSs containing DMAM-TBAM copolymer and four salts (sodium chloride, ammonium hydrogen phosphate, potassium hydrogen phosphate, and sodium carbonate) at T = 338.15 K are given in table 6. System 1 (copolymer + NH 4 H 2 PO 4 ) and system 2 (copolymer + KH 2 PO 4 ) are different in cation type.…”

“…Recently the partitioning of some series of proteins in ATPS containing pluronic block copolymers have been reported [5][6][7].…”

Applications

“…[212][213][214][215] The partitioning has been investigated, with the Pluronic in both the unimer and micellar states. The selective partitioning between aqueous and hydrophobic domains in two-phase systems with Pluronic and dextran polymer components leads to a distribution between the two phases depending on the hydrophobicity or hydrophilicity of the protein.…”