Partitioning of high-density lipoproteins in charge-sensitive two-phase systems

Wiegel, D; Meyer, Christine; Arnold, Katrin

doi:10.1016/0378-4347(94)00338-6

Cited by 2 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They indicated also that the large particle size of low-density lipoproteins leads to accumulation at the interface. 5,6 Marked similarity is seen between our and their results, though the sample systems are entirely different.…”

Section: ! ]L Isupporting

confidence: 81%

Adsorption and interface accumulation of aquasol and organosol carbon particles labeled with99mTc

Nozaki¹,

Satoh²,

Muraoka³

et al. 1999

J Radioanal Nucl Chem

View full text Add to dashboard Cite

Aerosol particles of 99mTc-labeled carbon were prepared by sublimation and introduced in various liquid media. The adsorption of the aquasol and organosol particles were studied for various adsorbing substances and media, with the effect of surface treatment and voltage application. The particles often accumulated at the aqueous-organic interface, and also on the vessel surface in the presence of both aqueous and organic phases.The distribution of the particles was examined by a gamma-camera for different organic phases under various concentrations of electrolytes in the aqueous phase.

show abstract

Section: ! ]L Isupporting

confidence: 81%

Adsorption and interface accumulation of aquasol and organosol carbon particles labeled with99mTc

Nozaki¹,

Satoh²,

Muraoka³

et al. 1999

J Radioanal Nucl Chem

View full text Add to dashboard Cite

show abstract

“…ATPS has great potential and versatility for the downstream processing of biopharmaceuticals such as monoclonal antibodies (Azevedo et al 2009), high density lipoproteins (Wiegel et al 1994), cytokines (Delgado et al 1994), and plasmid DNA (Ribeiro et al 2002). These systems combine a high biocompatibility, as both phases consists mainly of water, with an easy scale-up and continuous mode of operation (Albertsson et al 1990).…”

Section: Introductionmentioning

confidence: 98%

A novel method for human hematopoietic stem/progenitor cell isolation from umbilical cord blood based on immunoaffinity aqueous two-phase partitioning

et al. 2011

View full text Add to dashboard Cite

A novel cell separation process based on immunoaffinity aqueous two phase systems is presented to isolate and purify CD34(+) stem/progenitor cells directly from the whole umbilical cord blood (UCB). A system, composed of polyethylene glycol and dextran, was evaluated for the selective recovery of CD34(+) cells from UCB. A monoclonal antibody against the CD34 surface antigen was used for the direct partitioning of CD34(+) cells in UCB to the PEG-rich phase. The initial population of CD34(+) cells (0.2% of the initial sample) was enriched to values up to 42% in a single partitioning step, while the majority of contaminant cells were partitioned to the dextran-rich phase (1.37 × 10(-2) < K(P) < 2.76 × 10(-2)). This novel selection method allowed a recovery yield of 95% of CD34(+) cells with a purification factor of 245 and is expected to pave a new way to purify hematopoietic stem/progenitor cells for use in a variety of clinical settings.

show abstract

Partitioning of high-density lipoproteins in charge-sensitive two-phase systems

Cited by 2 publications

References 15 publications

Adsorption and interface accumulation of aquasol and organosol carbon particles labeled with99mTc

Adsorption and interface accumulation of aquasol and organosol carbon particles labeled with99mTc

A novel method for human hematopoietic stem/progenitor cell isolation from umbilical cord blood based on immunoaffinity aqueous two-phase partitioning

Contact Info

Product

Resources

About