Partitioning of cefazolin in aqueous two-phase systems containing poly (ethylene glycol) and sodium salts (citrate, tartrate, and sulphate)

Ghaffari, Somayyeh; Shahrouzi, Javad Rahbar; Towfighi, Farshid; Khoshfetrat, Ali Baradar

doi:10.1016/j.fluid.2019.01.010

Cited by 34 publications

(17 citation statements)

References 34 publications

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“…Furthermore, the solubility of HA in either PEG-rich or salt-rich environments also influences its partitioning. The pKa of the carboxyl groups in HA is between 3 and 4 (Dosio et al 2016 ); thus, at the pH of the salt-rich bottom phase (~ 8.0) (Ghaffari et al 2019 ), the carboxyl groups are deprotonated, making HA a polyanion with preference for the more hydrophilic phase, where ionic species are easily solvated by free water molecules. In addition, the partitioning of biomolecules in ATPS is also driven by electrostatic interactions between the biomolecule and the components of the two phases (Yang et al 2010 ).…”

Section: Discussionmentioning

confidence: 99%

“…ATPS were prepared by mixing determined weights of stock solutions of either PEG6000 (50% w/w) or PEG8000 (50% w/w) and sodium citrate (25% w/w) into clear, graduated 2-mL microcentrifuge tubes. The TLL and compositions of the ATPS were calculated based on the PEG–citrate binodal curves previously reported (Ghaffari et al 2019 ) and summarised in Table 1 . The sample was added to account for the 10% (w/w) of the ATPS, and the final mass of the ATPS was adjusted to 2.0 g with water.…”

Section: Methodsmentioning

confidence: 99%

“…ATPS, given their aqueous environment and mild conditions, have been largely employed for the recovery and separation of a broad range of biomacromolecules, including proteins, enzymes, antibodies, peptides, and genetic material (Asenjo and Andrews 2011 ; Azevedo et al 2009 ; Sánchez-Trasviña et al 2019 ), as well as low molecular weight compounds with biological activity (Enriquez-Ochoa et al 2020 ; Ghaffari et al 2019 ; Simental-Martínez et al 2014 ). Nevertheless, the application of ATPS for the separation of polysaccharides has been limited, mainly oriented as an extraction technique for polysaccharides from plant sources using ethanol-salt ATPS (Chen et al 2016 ; Cheng et al 2017 ; Wu et al 2017 ; Zhang et al 2018 ; Zhu et al 2020 ), whereas the implementation of ATPS in the downstream processing of polysaccharides from microbial fermentations is practically unexplored.…”

Section: Introductionmentioning

confidence: 99%

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Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

et al. 2021

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Given its biocompatibility, rheological, and physiological properties, hyaluronic acid (HA) has become a biomaterial of increasing interest with multiple applications in medicine and cosmetics. In recent decades, microbial fermentations have become an important source for the industrial production of HA. However, due to its final applications, microbial HA must undergo critical and long purification processes to ensure clinical and cosmetic grade purity. Aqueous two-phase systems (ATPS) have proven to be an efficient technique for the primary recovery of high-value biomolecules. Nevertheless, their implementation in HA downstream processing has been practically unexplored. In this work, polyethylene glycol (PEG)–citrate ATPS were used for the first time for the primary recovery of HA produced with an engineered strain of Streptococcus equi subsp. zooepidemicus. The effects of PEG molecular weight (MW), tie-line length (TLL), volume ratio (VR), and sample load on HA recovery and purity were studied with a clarified fermentation broth as feed material. HA was recovered in the salt-rich bottom phase, and its recovery increased when a PEG MW of 8000 g mol−1 was used. Lower VR values (0.38) favoured HA recovery, whereas purity was enhanced by a high VR (3.50). Meanwhile, sample load had a negative impact on both recovery and purity. The ATPS with the best performance was PEG 8000 g mol−1, TLL 43% (w/w), and VR 3.50, showing 79.4% HA recovery and 74.5% purity. This study demonstrated for the first time the potential of PEG–citrate ATPS as an effective primary recovery strategy for the downstream process of microbial HA.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

et al. 2021

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“…[6][7][8] These environmentally friendly ATPS have been already considered for the separation of biomolecules such as antibiotics, DNP-amino acids, among others. [9][10][11][12][13] Presence of organic salt and high content of water in such ATPS are advantageous. However, due to many dependencies of the partitioning behavior, understanding the phenomena which rule the partitioning in these complex systems requires much more effort (e.g., data) than in conventional separation systems.…”

Section: Introductionmentioning

confidence: 99%

“…A better alternative are biodegradable ATPS composed of nontoxic components, such as polyethylene glycol (PEG) and organic salts, for example, citrates or tartrates 6‐8 . These environmentally friendly ATPS have been already considered for the separation of biomolecules such as antibiotics, DNP‐amino acids, among others 9‐13 . Presence of organic salt and high content of water in such ATPS are advantageous.…”

Section: Introductionmentioning

confidence: 99%

Partitioning of water‐soluble vitamins in biodegradable aqueous two‐phase systems: Electrolyte perturbed‐chain statistical associating fluid theory predictions and experimental validation

et al. 2020

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Partition coefficients (K) of vitamins (riboflavin, nicotinic acid, nicotinamide, folic acid, cyanocobalamin) in aqueous two‐phase systems (ATPS) composed by polyethylene glycol (PEG 4000, PEG 6000) and organic salt (sodium citrate and sodium tartrate) at T = 298.15 K and p = 1 bar have been studied. Data on liquid–liquid equilibria of the ATPS considered in this study have been taken from the literature (PEG‐Na3Citrate) or measured in this work (PEG‐Na2Tartrate) for PEG 4000 and PEG 6000 at T = 298.15 K and p = 1 bar. The experimental K values were validated by electrolyte perturbed‐chain‐statistical associating fluid theory predictions. The neutral cyanocobalamin has the highest K values among all studied vitamins at any ATPS studied in this work. This finding contrasted with expectations based on literature data which let assume that charged species have typically the highest K values in the considered ATPS. Thus, besides the typically strong charge–charge interactions especially specific forces (e.g., hydrogen bonding) explains the strong PEG‐cyanocobalamin interaction resulting in the high K values.

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Water‐free two‐phase systems: phase equilibria and partitioning of vitamins from tomatoes (Solanum lycopersicum)

Lima,

Buarque,

Pinto

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUNDThe present work provides diagrams for water‐free two‐phase systems based on polypropylene glycol 2000 (PPG 2000) + [C2mim][OAc] + alcohols (primary: methanol, ethanol, 1‐propanol, and 1‐butanol; secondary: 2‐propanol and 2‐butanol; and tertiary: 2‐methyl‐2‐propanol.) at 298.15 ± 1.00 K and 0.10 ± 0.01 MPa. As a proof of concept, the systems were applied to study the selective partitioning of vitamins, namely β‐carotene, and α‐tocopherol.RESULTSThe hydrophilic/hydrophobic balance is the driving force for phase separation. The more hydrophilic alcohols are responsible for the wider biphasic area, and branching increases hydrophilicity and the biphasic area compared to linear alcohols. Additionally, it was verified that the mathematical determination of the tie‐lines satisfactorily describes the positioning in the phase diagram when compared with the analytical determination. In the proposed systems, pure biomolecules partitioned selectively, α‐tocopherol migrated to the PPG 2000 phase (top phase) while β‐carotene to [C2mim][OAc] phase (bottom phase). For the study with real biomolecules, solid‐liquid extraction was performed using 1‐butanol (more selective alcohol) in a solid‐liquid ratio of 1:10 at 200 rpm for 60 min. The partitioning trend followed the behavior of systems using pure biomolecules. The highest selectivity (5.3) was observed for the system composed of PPG 2000 + [C2mim][OAc] + 1‐butanol or 2‐butanol. The partition system did not impair the antioxidant potential expressed in DPPH inhibition.CONCLUSIONSWater‐free two‐phase systems can be formed and applied to partitioning vitamins from tomatoes.This article is protected by copyright. All rights reserved.

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Partitioning of cefazolin in aqueous two-phase systems containing poly (ethylene glycol) and sodium salts (citrate, tartrate, and sulphate)

Cited by 34 publications

References 34 publications

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

Partitioning of water‐soluble vitamins in biodegradable aqueous two‐phase systems: Electrolyte perturbed‐chain statistical associating fluid theory predictions and experimental validation

Water‐free two‐phase systems: phase equilibria and partitioning of vitamins from tomatoes (Solanum lycopersicum)

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