Particulate β‐glucan induces TNF‐α production in wound macrophages via a redox‐sensitive NF‐κβ‐dependent pathway

Roy, Sashwati; Dickerson, Ryan; Khanna, Savita; Collard, Eric; Gnyawali, Urmila; Gordillo, Gayle M.; Sen, Chandan K.

doi:10.1111/j.1524-475x.2011.00688.x

Cited by 29 publications

(27 citation statements)

References 40 publications

(42 reference statements)

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“…The NPWT dressing (sponges) were collected from each patient for cell isolation and wound fluid collection. Wound fluids were derived from NPWT dressing by lavaging the wound dressing with saline solution (18). All human studies were approved by The Ohio State University Institutional Review Board.…”

Section: Methodsmentioning

confidence: 99%

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Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes

Das

Ghatak

Sinha

et al. 2016

The Journal of Immunology

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109

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Milk fat globule EGF factor 8 (MFG-E8) is a peripheral glycoprotein which acts as a bridging molecule between the macrophage and apoptotic cells thus executing a pivotal role in the scavenging of apoptotic cells from affected tissue. We have previously reported that apoptotic cell clearance activity or efferocytosis is compromised in diabetic wound macrophages. In this work we test the hypothesis that MFG-E8 helps resolve inflammation, supports angiogenesis and accelerates wound closure. MFG-E8−/− mice, displayed impaired efferocytosis associated with exaggerated inflammatory response, poor angiogenesis and wound closure. Wound macrophage-derived MFG-E8 was recognized as a critical driver of wound angiogenesis. Transplantation of MFG-E8−/− bone marrow to MFG-E8+/+ mice resulted in impaired wound closure and compromised wound vascularization. On the other hand, MFG-E8−/− mice that received wild-type bone marrow showed improved wound closure and improved wound vascularization. Hyperglycemia and exposure to advanced glycated end products inactivated MFG-E8 recognizing a key mechanism that complicates diabetic wound healing. Diabetic db/db mice suffered from impaired efferocytosis accompanied with persistent inflammation and slow wound closure. Topical rMFG-E8 induced resolution of wound inflammation, improvements in angiogenesis and acceleration of closure upholding the potential of MFG-E8 directed therapeutics in diabetic wound care.

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Section: Methodsmentioning

confidence: 99%

“…mirVana RNA isolation kit (Ambion, Austin, TX) was used according to the manufacturer’s instructions to extract total RNA as previously described (18). Quantification of mRNA was done by real-time or quantitative (Q)PCR assay using double-stranded DNA binding dye SYBR Green-I, as described previously(21, 32, 35).…”

Section: Methodsmentioning

confidence: 99%

Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes

Das

Ghatak

Sinha

et al. 2016

The Journal of Immunology

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109

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“…Data are expressed as mean -SD (n = 3) *p < 0.05 compared to non-FPP CTL. by real-time polymerase chain reaction assay using SYBR green-I (Applied Biosystems, Carlsbad, CA) as described previously (36,(38)(39)(40). b-Actin was used as the housekeeping gene.…”

Section: Figmentioning

confidence: 99%

“…density centrifugation followed by sorting with anti-CD14 coated magnetic microbeads (Miltenyi Biotech, Auburn, CA) as previously described (36). Purified CD14 + PBMCs were then cultured under standard conditions (5% CO 2 , 37°C, humidified incubator).…”

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confidence: 99%

Correction of Aberrant NADPH Oxidase Activity in Blood-Derived Mononuclear Cells from Type II Diabetes Mellitus Patients by a Naturally Fermented Papaya Preparation

Dickerson

Deshpande

Gnyawali

et al. 2012

Antioxidants & Redox Signaling

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Supplementation of standardized fermented papaya preparation (FPP) to adult diabetic mice improves dermal wound healing outcomes. Peripheral blood mononuclear cells (PBMC) from type II diabetes mellitus (T2DM) patients elicit a compromised respiratory burst activity resulting in increased risk of infections for the diabetic patients. Aims: The objectives of the current study were to determine the effect of FPP supplementation on human diabetic PBMC respiratory burst activity and to understand underlying mechanisms of such action of FPP. Results: When stimulated with phorbol 12-myristate 13-acetate, the production of reactive oxygen species by T2DM PBMC was markedly compromised compared to that of the PBMC from non-DM donors. FPP treated ex vivo improved respiratory burst outcomes in T2DM PBMC. FPP treatment significantly increased phosphorylation of the p47phox subunit of NADPH oxidase. In addition, the protein and mRNA expression of Rac2 was potently upregulated after FPP supplemention. The proximal human Rac2 gene promoter is G-C rich and contains consensus binding sites for Sp1 and AP-1. While FPP had no significant effect on the AP-1 DNA binding activity, the Sp1 DNA binding activity was significantly upregulated in PBMC after treatment of the cells with FPP. Innovation: This work provided first evidence that compromised respiratory burst performance of T2DM PBMC may be corrected by a nutritional supplement. Conclusion: FPP can correct respiratory burst performance of T2DM PBMC via an Sp-1-dependant pathway. Studies testing the outcome of FPP supplementation in diabetic patients are warranted. Antioxid. Redox Signal. 17, 485-491.

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“…CLEC7A (dectin 1) is a molecule promoting wound healing by the enhanced production of collagen matrices with beta-glucans 63–65 . Our results show that dectin 1 is about four times over-expressed in coiled IA when compared to flow-diverter treated IA.…”

Section: Discussionmentioning

confidence: 99%

Differential Gene Expression in Coiled versus Flow-Diverter-Treated Aneurysms: RNA Sequencing Analysis in a Rabbit Aneurysm Model

Rouchaud¹,

Johnson²,

Thielen³

et al. 2015

AJNR Am J Neuroradiol

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Introduction The biological mechanisms leading to aneurysm healing or rare complications such as delayed aneurysm ruptures after flow-diverter placement remain poorly understood. We used RNA-sequencing (RNA-seq) following implantation of coils or flow-diverters in elastase aneurysms in rabbits to identify genes and pathways of potential interest. Methods Aneurysms were treated with coils (n=5) or flow-diverters (n=4) or left untreated for controls (n=6). Messenger RNA were isolated from the aneurysms at 4 weeks following treatment. RNA samples were processed using RNA-seq technology and analyzed using the Ingenuity Pathway Analysis tool. Results Using RNA-seq for coiled versus untreated aneurysms, 464/9990 genes (4.6%) were differentially expressed (58 down-regulated, 406 up-regulated). Comparing flow-diverter versus untreated aneurysms, 177/10041 (1.8%) genes were differentially expressed (8 down-regulated, 169 up-regulated). Comparing flow-diverter versus coiled aneurysms, 13/9982 (0.13%) genes were differentially expressed (8 down-regulated, 5 up-regulated). Keratin 8 was overexpressed in flow-diverters versus coils. This molecule may potentially play a critical role in delayed ruptures due to plasmin production. We identified overregulation of apelin in flow-diverters supporting the preponderance of endothelialization, whereas we found overexpression of molecules implicated in wound healing (Dectin1 and HHIP) for coiled aneurysms. Furthermore, we identified metallopeptidases 1, 12 and 13 as overexpressed in coiled versus untreated aneurysms. Conclusions We observed different physiopathologic responses after endovascular treatment with different devices. Flow-diverters promote endothelialization but express molecules that could potentially explain the rare delayed ruptures. Coils promote wound healing and express genes potentially implicated in recurrence of coiled aneurysms.

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Particulate β‐glucan induces TNF‐α production in wound macrophages via a redox‐sensitive NF‐κβ‐dependent pathway

Cited by 29 publications

References 40 publications

Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes

Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes

Correction of Aberrant NADPH Oxidase Activity in Blood-Derived Mononuclear Cells from Type II Diabetes Mellitus Patients by a Naturally Fermented Papaya Preparation

Differential Gene Expression in Coiled versus Flow-Diverter-Treated Aneurysms: RNA Sequencing Analysis in a Rabbit Aneurysm Model

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