Particulate Nature of Vibriocin: a Bacteriocin from Vibrio comma

Jayawardene, A; Farkas-Himsley, Hannah

doi:10.1038/219079a0

Cited by 23 publications

(7 citation statements)

References 4 publications

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“…The cytotoxic effect on cancerous cells from human origin was also reported earlier [31]. The uniqueness of the bacteriocins lies in their interaction with the cell surface without penetrating the target cells, yet affecting cell division and DNA synthesis [32]. Bacteriocins are highly specific in their membrane interaction which is related to the unique receptors found in different bacterial species or types [33].…”

Section: Discussionsupporting

confidence: 54%

In Vitro Cytotoxicity of Native and Rec-Pediocin CP2 Against Cancer Cell Lines: A Comparative Study

Kumar¹,

Balgir²,

Kaur³

et al. 2012

Pharm Anal Acta

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Pediocin CP2 is a natural antimicrobial peptide produced by Pediococcus acidilactici MTCC 5101. Its recombinant version was created by employing computational protein engineering approach and was expressed as a synthetic fusion protein in recombinant E. coli BL21(DE3)-pedA. Both native and rec-pediocin were comparatively evaluated for their cytotoxicity against HepG2 (a hepatocarcinoma cell line), HeLa (a cervical adenocarcinoma), MCF7 (a mammary gland adenocarcinoma) and Sp2/0-Ag14 (a spleen lymphoblast) cell lines. Inhibition of cell proliferation was quantitated by MTT assay and induction of apoptosis was studied by genomic DNA fragmentation assay. Results indicated a significantly higher cytotoxicity of rec-pediocin and damage of chromosomal DNA in bacteriocin tested cell lines.

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Section: Discussionsupporting

confidence: 54%

In Vitro Cytotoxicity of Native and Rec-Pediocin CP2 Against Cancer Cell Lines: A Comparative Study

Kumar¹,

Balgir²,

Kaur³

et al. 2012

Pharm Anal Acta

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“…On turning to other groups of bacteria, and particularly to gram-positive genera, the inhibition zones seen on agar plates are more likely to be due to various unrelated inhibitors. The results of surveys which measure the incidence of "bacteriocinogeny" in bacteria other than members of the Enterobacteriaceae are particularly difficult to assess in view of the reports of bacte-riocin-like action due to such diverse agents as ammonia (482), hydrogen peroxide (264,389), lactic acid (567), phospholipases (including clostridial a-toxin) (114,455), hemolysins (58,259), the lytic enzyme lysostaphin (501), bacteriophage tails or defective bacteriophages (49,142,298,308,496), phospholipids, and fatty acids (580).…”

Section: Colicins and Diseasementioning

confidence: 99%

Colicinogeny and related phenomena.

Hardy¹

1975

Bacteriological Reviews

130

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“…Purified preparations of some large bacteriocins consist of phagelike particles; several colicins (4, 28, 36), pyocins (3,13,16), monocins (4,12), and a vibriocin (22,23) are included in this group.…”

Section: As Bradleymentioning

confidence: 99%

Purification and Some Properties of Two Boticins

Ellison

Kautter

1970

J Bacteriol

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Two bacteriocins (boticins) were elaborated without induction by strain S5, a nontoxigenic variant of Clostridium botulinum type E. After separation of the two active entities by gel filtration on Sephadex G-50, a large particle with boticin activity was isolated by density gradient ultracentrifugation, and a small soluble boticin was purified by continuous curtain electrophoresis and chromatography on sulfoethyl-Sephadex. Large and small boticins were purified 200-and 3,000-fold, respectively, with yields of 50% or more. The small boticin, a basic substance with molecular weight under 30,000, was the predominant species; the large boticin, a negatively charged particle with particle weight greater than 40 x 106, represented less than 20% of the total activity. Both purified boticins were resistant to heat and were attacked by proteolytic enzymes, but the large boticin was less thermostable and less sensitive to proteolytic enzymes than was the smaller variety. The activity of the large boticin was not reduced by treatment with urea or deoxyribonuclease. Both boticins exhibited sporostatic and bactericidal activities for C. botulinum type E, strain 070. A suspension of type E strain 070 vegetative cells was rendered nonviable within 9 min by the small boticin. The lethal action of this bacteriocin was not reversed by trypsin.The production of bacteriocins (boticins) active against toxigenic Clostridium botulinum type E strains by several closely related but nontoxigenic organisms was described in an earlier report from this laboratory (25). Since the initial report, we have detected (unpublished data) bacteriocin activity against C. botulinum type E in filtrates of C. perfringens and C. botulinum types A, B, and E strains as well. Interference of these bacteriocin-producing organisms with methods for the detection or recovery (or both) of toxin-producing type E has been demonstrated (25).The status of our knowledge regarding the bacteriocins has been reviewed frequently (3,9,10,17,21,30,34); the recent reviews of Reeves (34) and Bradley (3) are especially pertinent. Bradley's broad definition of bacteriocins as "bactericidal particles which are unable to multiply in a sensitive indicator" is applied in this report.Many bacteriocinogenic organisms elaborate maximal quantities of bacteriocins only after induction with ultraviolet light, metabolic inhibitors such as mitomycin C, or other agents known to induce lysogenic phages (2,10,13,18,20,24,34,37). Some organisms release bacteriocin continuously (11,26); in other cases, an abrupt release of bacteriocin coincides with partial or complete lysis of the culture (11,14,17,20,24).As Bradley (3) pointed out, the bacteriocins studied to date fall into two distinct categories. One group consists of bacteriocins of small molecular size, which are usually thermostable and cannot be sedimented by ultracentrifugation; bacteriocins of very large size, which are usually thermolabile and can be sedimented easily, make up the second group. The production of a bacteriocin of each...

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Particulate Nature of Vibriocin: a Bacteriocin from Vibrio comma

Cited by 23 publications

References 4 publications

In Vitro Cytotoxicity of Native and Rec-Pediocin CP2 Against Cancer Cell Lines: A Comparative Study

In Vitro Cytotoxicity of Native and Rec-Pediocin CP2 Against Cancer Cell Lines: A Comparative Study

Colicinogeny and related phenomena.

Purification and Some Properties of Two Boticins

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