Particulate malate oxidation in strictly aerobic bacteria: the respiratory system of <i>Moraxella lwoffi</i>

Jones, Merfyn; King, Hugh K.

doi:10.1042/bj1270089pa

Cited by 3 publications

(2 citation statements)

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“…We have confirmed this report and, furthermore, have demonstrated the requirement of this enzyme for FAD and its association with the electron transport chain of N. gonorrhoeae. In this respect, the enzyme appears similar to those reported for Azotobacter vinelandii (26) and Moraxella iwoffi (25). However, further studies are required to firmly establish the relation of this enzyme to electron transport as well as its potential involvement in oxidative phosphorylation in N. gonorrhoeae.…”

Section: Discussionsupporting

confidence: 76%

Physiology and metabolism of pathogenic neisseria: tricarboxylic acid cycle activity in Neisseria gonorrhoeae

Hebeler¹,

Morse²

1976

J Bacteriol

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Tricarboyxlic acid cycle activity was examined in Neisseria gonorrhoeae CS-7. The catabolism of glucose in N. gonorrheae by a combination of the Entner-Doudoroff and pentose phosphate pathways resulted in the accumulation of acetate, which was not further catabolized until the glucose was depleted or growth became limiting. Radiorespirometric studies revealed that the label in the 1 position of acetate was converted to CO2 at twice the rate of the label in the 2 position, indicating the presence of a tricarboxylic acid cycle. Growth on glucose markedly reduced the levels of all tricarboxylic acid cycle enzymes except citrate synthase (EC 4.1.3.7). Extracts of glucose-grown cells contained detectable levels of all tricarboxylic acid cycle enzymes except aconitase (EC 4.2.1.3), isocitrate dehydrogenase (EC 1.1.1.42), and a pyridine nucleotide-dependent malate dehydrogenase (EC 1.1.1.37). Extracts of cells capable of oxidizing acetate lacked only the pyridine nucleotide-dependent malate dehydrogenase. In lieu of this enzyem, a particulate pyridine nucleotide-independent malate oxidase (EC 1.1.3.3) was present. This enzyme required flavin adenine dinucleotide for activity and appeared to be associated with the electron transport chain. Radiorespirometric studies utilizing labeled glutamate demonstrated that a portion of the tricarboxylic acid cycle functioned during glucose catabolism. In spite of the presence of all tricarboxylic acid cycle enzymes, N. gonorrhoeae CS-7 was unable to grow in medium supplemented with cycle intermediates.

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Section: Discussionsupporting

confidence: 76%

Physiology and metabolism of pathogenic neisseria: tricarboxylic acid cycle activity in Neisseria gonorrhoeae

Hebeler¹,

Morse²

1976

J Bacteriol

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“…Jones et al (1977) noted that true molar growth yields of bacteria are not significantly altered by the major cytochrome oxidase moiety but are influenced by the presence or absence of a high-potential, membrane-bound cytochrome c; this is a redox carrier which has not been detected in Acinetobacter spp. (this paper; also Whittaker, 1971 ;Jones & King, 1972;Asperger et al, 1978;Ensley & Finnerty, 1980).…”

Section: Discussionmentioning

confidence: 76%

Effect of Oxygen Concentration on the Growth and Respiratory Efficiency of Acinetobacter calcoaceticus

Hardy

Dawes

1985

Microbiology

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Maximum molar growth yields on oxygen and succinate (YE;inate) were not affected when carbon-limited Acinetobacter calcoaceticus NCIB 8250 was changed to oxygen limitation. +H+/O quotients for cells grown with succinate-or ammonium-limitation did not differ significantly from those obtained with oxygen-limited cells. The levels of cytochromes b and o remained constant at 0.13 and 0.05 nmol (mg protein)-' respectively in crude cell-free extracts of succinate-limited organisms grown at high (80 mmHg) or low (5-10 mmHg) dissolved oxygen tension respectively. Their levels increased in oxygen-limited conditions, when cytochrome a2 (d) was additionally synthesized and organisms exhibited increased resistance to cyanide inhibition of respiration. It is concluded that the respiratory efficiency of A. calcoaceticus NCIB 8250 is unaffected by oxygen concentration under the conditions studied. Theoretical calculations are presented for maximum molar growth yields on succinate and oxygen and these are compared with experimental values. Although the effective P/O ratio is only about unity, energy is probably conserved at two sites of oxidative phosphorylation and this is independent of the de novo synthesis of cytochrome a2 (d) under conditions of oxygen limitation. Possible reasons for the observed low molar growth yields are discussed.

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Moraxella, Acinetobacter, and the Mimeae

Henriksen¹

1973

Bacteriol Rev

118

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Particulate malate oxidation in strictly aerobic bacteria: the respiratory system of Moraxella lwoffi

Cited by 3 publications

References 1 publication

Physiology and metabolism of pathogenic neisseria: tricarboxylic acid cycle activity in Neisseria gonorrhoeae

Physiology and metabolism of pathogenic neisseria: tricarboxylic acid cycle activity in Neisseria gonorrhoeae

Effect of Oxygen Concentration on the Growth and Respiratory Efficiency of Acinetobacter calcoaceticus

Moraxella, Acinetobacter, and the Mimeae

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