Particle tracking microrheology of purified gastrointestinal mucins

Georgiades, Pantelis; Pudney, Paul D. A.; Thornton, David J.; Waigh, Thomas A.

doi:10.1002/bip.22372

Cited by 115 publications

(163 citation statements)

References 39 publications

(67 reference statements)

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“…The results were consistent with the research of Pantelis Georgiades et al, who reported that purified stomach mucin solutions of up to 20 mg/mL behaved as viscous liquids and the sol−gel transition concentration was 25 mg/mL. 22 Moreover, the viscosity of normal gastric mucus decreased from 300 mPa·s to 25 mPa·s, with the shear rate ranging from 1.15 s −1 to 115 s −1 . 23 The viscosities of normal gastric mucus and porcine gastric mucin solution had the same order of magnitude.…”

Section: Resultssupporting

confidence: 92%

Influence of Microemulsion–Mucin Interaction on the Fate of Microemulsions Diffusing through Pig Gastric Mucin Solutions

Zhang

Wang

et al. 2015

Mol. Pharmaceutics

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Mucus layer, a selective diffusion barrier, has an important effect on the fate of drug delivery systems in the gastrointestinal tract. To study the fate of microemulsions in the mucus layer, four microemulsion formulations with different particle sizes and lipid compositions were prepared. The microemulsion-mucin interaction was demonstrated by the fluorescence resonance energy transfer (FRET) method. Moreover, the microemulsions were observed aggregated into micron-sized emulsions by laser confocal microscopy. We concluded the microemulsion-mucin interaction not only led to microemulsions closely adhered to mucins but also destroyed the structure of microemulsions. At last, the diffusion of blank microemulsions and microemulsion-carried drugs (resveratrol and hymecromone) through mucin solutions was determined by the fluorescence recovery after photobleaching (FRAP) method and the Franz diffusion cell method. The results demonstrated the diffusion of microemulsions was significantly hindered by mucin solutions. The particle size of microemulsions had a negligible effect on the diffusion coefficients. However, the type of lipid played an important role, which could form hydrophobic interactions with mucins. Interestingly, microemulsion-carried drugs with different core/shell locations seemed to suffer different fates in the mucin solutions. The drug incorporated in the oil core of microemulsions, resveratrol, was transported through the mucus layer by the carriers, while the drug incorporated in the surfactant shell of microemulsions, hymecromone, was separated from the carriers and diffused toward the epithelium in the form of free molecules.

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Section: Resultssupporting

confidence: 92%

Influence of Microemulsion–Mucin Interaction on the Fate of Microemulsions Diffusing through Pig Gastric Mucin Solutions

Zhang

Wang

et al. 2015

Mol. Pharmaceutics

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“…2.C shows the loss modulus, G" (ω). Comparing PGM at the two concentrations without bacteria, we note that at both concentrations G'< G" indicating that the mucin at pH 6 is solution at both concentrations, in agreement with the results of Celli et al [5] and Georgiades et al [25]. As expected both moduli are larger in the more concentrated solution.…”

Section: Average Mean Squared Displacement G' and G'' For 1 µM Besupporting

confidence: 92%

Microrheology of Mucin: Tracking Particles and Helicobacter Pylori Bacteria

Bansil¹,

Hardcastle²,

Constantino³

2015

Epitoanyag - JSBCM

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the gastric ulcer and cancer causing bacteria, helicobacter Pylori have uniquely adapted to swim across the viscoelastic mucus gel that lines the stomach epithelial surface and colonize in the harsh acidic environment of the stomach. in this paper we first briefly review results of bacteria tracking and oscillatory shear rheology studies to suggest how the bacteria get across the viscoelastic mucus gel by using a chemical mechanism to raise the pH from acidic to neutral which also triggers a gel to sol transition of mucin. We then present new microrheology studies to show that the bacterium influences the Brownian motion of spherical tracer particles in culture broth solution and in solutions of gastric mucin. the elastic and viscous moduli obtained by tracking particles in the mucin solutions are found to decrease in the presence of bacteria. We also examined the Brownian motion of the bacteria themselves and find that motile bacteria display super-diffusive anomalous Brownian motion while the immotile bacteria exhibit regular diffusive Brownian motion.

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“…The problem with this is that it is a chaotropic agent and will denature the mucins and reduce their ability to form a gel. Studies by Georgiades et al have shown that GuHCl disassembles the semi dilute network structure of porcine gastric mucin solutions by unfolding proteins and disrupting non-covalent interactions [17]. In studies where it is necessary to visualize particles in a scraped mucus gel or the gel contains food particles and other debris, the gel can be cleaned up by gently stirring for one hour at 4 °C in sodium chloride (0.1M) (1:5 w/v mucus to sodium chloride) and then centrifuged (10,400g at 10 °C) for 2 hours.…”

Section: Intestinal Mucus Collection and Purificationmentioning

confidence: 99%