Particle Sizing by Photon Correlation Spectroscopy Part I: Monodisperse latices: Influence of scattering angle and concentration of dispersed material

Jaeger, Nicolas De; Demeyere, Hugo; Finsy, Robert; Sneyers, Rik; Vanderdeelen, Jean; Meeren, Paul Van der; Laethem, Marc Van

doi:10.1002/ppsc.19910080134

Cited by 78 publications

(34 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A sample of the diluted suspension is illuminated with a laser beam. The scattered light on the dispersed particles is registered at an angle θ in relation to the incident beam [64]. Because particles in a suspension undergo continuous Brownian motion, the intensity of scattered light changes over time.…”

Section: Protein Adsorption Investigationsmentioning

confidence: 99%

See 1 more Smart Citation

Characterization methods of polymer core–shell particles

Gosecka

Gosecki

2015

Colloid Polym Sci

View full text Add to dashboard Cite

The design and synthesis of various polymer coreshell particles result from their distinct characteristics, which combine the properties of two or more components into one material. Many accessible synthetic strategies for obtaining polymer core-shell particles lead to the formation of particles for which the internal morphology differs from the ideal coreshell structure. Understanding the precise morphology characteristics is important for mechanistic studies of particle formation, which ultimately results in the design of particles for specific structures and properties. The detailed characteristics of complex polymer particle structures are complicated and require more than one method. This review focuses on imaging methods such as transmission electron microscopy (TEM), cryo-TEM, scanning transmission electron microscopy (STEM) and confocal fluorescence microscopy that reveal the radial redistribution of the components and methods for the quantitative analysis of individual phases (core, shell and interfacial layer), such as small-angle X-ray scattering (SAXS), small-angle neutron scattering (SANS), differential scanning calorimetry (DSC) and nuclear magnetic resonance (NMR). Methods that can determine the surface composition and makeup of the character of interfacial layer (gradient or containing small domains, etc.) were also reviewed.

show abstract

Section: Protein Adsorption Investigationsmentioning

confidence: 99%

“…The diffusion coefficient of a particle is then determined. Using the Stokes-Einstein equation [64], the average hydrodynamic diameter of the particle can be calculated.…”

Section: Protein Adsorption Investigationsmentioning

confidence: 99%

Characterization methods of polymer core–shell particles

Gosecka

Gosecki

2015

Colloid Polym Sci

View full text Add to dashboard Cite

show abstract

“…The effects of using a different lecithin (DMPC instead of S100) and PPP-free cLNP suspensions as a reference were studied, too. 35,36 Values d PCS 4 1 mm were not considered meaningful and are marked with a *-sign in Table SIV(a)-(c) (ESI †) and in Fig. 6.…”

Section: Clnp-dna Complexesmentioning

confidence: 99%

Analysis of the structure of nanocomposites of triglyceride platelets and DNA

Schmiele

Knittel

Unruh

et al. 2015

Phys. Chem. Chem. Phys.

View full text Add to dashboard Cite

DNA-complexes with platelet-like, cationically modified lipid nanoparticles (cLNPs) are studied with regard to the formation of nanocomposite structures with a sandwich-like arrangement of the DNA and platelets. For this purpose suspensions of platelet-like triglyceride nanocrystals, stabilized by a mixture of two nonionic (lecithin plus polysorbate 80 or poloxamer 188) and one cationic stabilizer dimethyldioctadecylammonium (DODAB), are used. The structure of the platelets in the native suspensions and their DNA-complexes, ranging from the sub-nano to the micron scale, is investigated with small-and wideangle scattering (SAXS, SANS, WAXS), calorimetry, photon correlation spectroscopy, transmission electron microscopy and computer simulations. The appearance of strong, lamellarly ordered peaks in the SAXS patterns of the DNA-complexes suggests a stacked arrangement of the nanocrystals, with the DNA being partially condensed between the platelets. This finding is supported with computer simulated small-angle scattering patterns of nanocrystal stacks, which can reproduce the measured small-angle scattering patterns on an absolute scale. The influence of the choice of the nonionic stabilizers and the amount of the cationic stabilizer DODAB on the structure of the native suspensions and the inner structure of their DNA-complexes is studied, too. Using high amounts of DODAB, lecithins with saturated acyl chains and polysorbate 80 instead of poloxamer 188 produces thinner nanocrystals, and thus decreases their repeat distances in the nanocomposites. Such nanocomposites could be of interest as DNA carriers, where the triglyceride platelets protect the sandwiched DNA from degradation.

show abstract

“…PCS is mainly based on the principle of laser light scattering by particles in colloidal dispersion [38]. It can be used for size measurement between 5 nm and 2 μ m. The size or diameter obtained by PCS is expressed as the hydrodynamic diameter -a value that refers to how a particle diffuses within a fl uid [39].…”

Section: Dynamic Light Scattering (Dls)/photon-correlation Spectroscopymentioning

confidence: 99%