Particle size distribution of the major Alternaria alternata allergen, Alt a 1, derived from airborne spores and subspore fragments

Grewling, Łukasz; Bogawski, Paweł; Szymańska, Agata; Nowak, Małgorzata; Kostecki, Łukasz; Smith, Matt

doi:10.1016/j.funbio.2020.02.005

Cited by 22 publications

(15 citation statements)

References 81 publications

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“…Despite there being a lot of studies about airborne allergens, these researches were focused on allergens from pollen and not from fungal spores. Most studies of aeroallergens from fungal spores were based on the detection and quantification of Alt a 1 [61,62,68,73] because it is the most important fungal aeroallergen that provokes respiratory tract symptoms. These studies were also helped by Elisa kits being available on the market for detection and quantification.…”

Section: Discussionmentioning

confidence: 99%

Airborne Alt a 1 Dynamic and Its Relationship with the Airborne Dynamics of Alternaria Conidia and Pleosporales Spores

Linares

Navarro

Puigdemunt

et al. 2022

JoF

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Fungal spores are universal atmospheric components associated to allergic reactions. Alternaria (Ascomycota) is considered the most allergenic spore taxa. Alt a 1 is the major allergen of Alternaria and is present also in other Pleosporales. In this study, standard Hirst-based sampling and analyzing methods for measuring spore daily concentrations of Alternaria, Curvularia, Drechslera-Helminthosporium, Epicoccum, Leptosphaeria, Pithomyces, Pleospora and Stemphylium (all included in the taxon Pleosporales) have been used as well as two high-volume samplers, Burkard Cyclone (2017) and MCV CAV-A/mb (2019–2020), and ELISA kits for measuring the allergen. The detection and quantification of Alt a 1 was only possible in the samples from the MCV sampler. Although Alt a 1 was better correlated with Alternaria spores than with Pleosporales spores, the three of them showed high correlations. It is shown that there is a high and significant correlation of Alt a 1 with temperature, a negative correlation with relative humidity and no correlation with precipitation. The aerobiological monitoring of these three elements ensures the best information for understanding the affectation to allergy sufferers, but, if this is not possible, as a minimum public health service aimed at the detection, treatment and prevention of allergies, the study of the airborne Alternaria spores should be ensured.

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Section: Discussionmentioning

confidence: 99%

Airborne Alt a 1 Dynamic and Its Relationship with the Airborne Dynamics of Alternaria Conidia and Pleosporales Spores

Linares

Navarro

Puigdemunt

et al. 2022

JoF

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“…In outdoor air the main source of the major allergen Alt a 1 are intact Alternaria spores, but also the quantity of other fungal fragments (broken spores, conidiophores, hyphal fragments, Pady andKramer 1960, Green et al 2005a). Consequently, the impact of these particles should not be overlooked, as they could increase the total atmospheric concentration of Alt a 1 (Green et al 2005b, Grewling et al 2020.…”

Section: Discussionmentioning

confidence: 99%

Airborne fungal spore monitoring: between analyst proficiency testing

et al. 2021

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This study presents the results of a Europe-wide training and Quality Control (QC) exercise carried out within the framework of the European Aerobiology Society's QC Working Group. The main aim of this exercise was to examine the feasibility of carrying out a QC exercise for fungal spore monitoring in Europe, using a similar methodology to the one previously used for pollen.The QC survey was conducted in two parts: (1) Coordinators of national and regional aerobiological networks in Europe involved in the monitoring of atmospheric fungal spores were invited to complete a questionnaire survey related to their network and asked whether they were interested in taking part in an external inter-laboratory QC exercise; (2) Participating networks performed an inter-laboratory ring test with the same sample slide in order to determine the reproducibility of identifying and counting two fungal spore taxa (Alternaria and Epicoccum) in air samples collected by a Hirst-type volumetric spore trap. Participants were instructed to read five separate longitudinal transects in the "effective collecting area" of the slide. Reproducibility of analysis was determined following the method previously used in the European Aerobiology Society's QC exercises for pollen. Thirty-two counters from 16 national or regional networks in Europe participated in the QC exercise. Coefficients of Variation (CV%)

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“…Despite that there are a lot of studies about airborne allergens, these researches were focused on allergens from pollen and not from fungal spores. Most studies of aeroallergens from fungal spores were based in the detection and quantification of Alt a 1 [61][62]68,73] because it is the most important fungal aeroallergen that provokes respiratory tract symptoms. It helped also to these studies that Elisa kits are available on the market for its detection and quantification.…”

Section: Discussionmentioning

confidence: 99%

Airborne Alt a 1 Dynamic and Its Relationship With the Airborne Dynamics of <em>Alternaria</em> Conidia and Pleosporales Spores

Linares¹,

Navarro²,

Puigdemunt³

et al. 2021

Preprint

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Fungal spores are universal atmospheric components associated to allergic reactions. Alternaria (Ascomycota) is considered the most allergenic spore taxa. Alt a 1 is the major allergen of Alternaria and is present also in other Pleosporales. In this study, standard Hirst-based sampling and analyzing methods for measuring spore daily concentrations of Alternaria, Curvularia, Drechslera-Helminthosporium, Epicoccum, Leptosphaeria, Pithomyces, Pleospora and Stemphyllium (all included in the taxon Pleosporales) have been used besides two high-volume samplers, Burkard Cyclone (2017) and MCV CAV-A/mb (2019-2020), and ELISA Kits for measuring the allergen. The detection and quantification of Alt a 1 was only possible in the samples from the MCV sampler. Although Alt a 1 was better correlated with Alternaria spores than with Pleosporales spores, the three of them showed high correlations. It is shown, for the first time, a high and significant correlation of Alt a 1 with temperature, a negative one with relative humidity and no correlation with precipitation. The aerobiological monitoring of these three elements ensures the best information for understanding the affectation to allergy sufferers but, if not possible, as a minimum public health service aiming at the detection, treatment and prevention of allergy, the study of the airborne Alternaria spores should be ensured.

show abstract

Particle size distribution of the major Alternaria alternata allergen, Alt a 1, derived from airborne spores and subspore fragments

Cited by 22 publications

References 81 publications

Airborne Alt a 1 Dynamic and Its Relationship with the Airborne Dynamics of Alternaria Conidia and Pleosporales Spores

Airborne Alt a 1 Dynamic and Its Relationship with the Airborne Dynamics of Alternaria Conidia and Pleosporales Spores

Airborne fungal spore monitoring: between analyst proficiency testing

Airborne Alt a 1 Dynamic and Its Relationship With the Airborne Dynamics of <em>Alternaria</em> Conidia and Pleosporales Spores

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