Particle Induced X-ray Emission (PIXE): A Tool of Qualitative Elemental Analysis for Biological Sample

Kabir, M. Hasnat

doi:10.3329/rujs.v39i0.16538

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…In PIXE, the samples are bombarded with particles (generally 1-4 MeV protons) accelerated in an accelerator and the characteristic X-rays produced by the de-excitation of the atoms in the sample are measured using semiconductor detector. Interaction of protons with matter generates the X-ray spectrum; from these spectra, elements as well as their concentrations can be determined in the sample [28].…”

Section: Pixe (Particle-inducedx-ray Emission) Analysismentioning

confidence: 99%

Plant Growth-Promoting Rhizobacteria (PGPR) Reduce Adverse Effects of Salinity and Drought Stresses by Regulating Nutritional Profile of Barley

Zaib,

Zubair,

Abbas

et al. 2023

Applied and Environmental Soil Science

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With the growing emphasis on sustainable agriculture, food security, and environmental protection, the use of beneficial soil microbes is imperative, as the use of chemicals such as fertilizers, pesticides, and herbicides has resulted in food contamination, disease, weed resistance, and negative environmental consequences, which ultimately impacted human health. Climate change is a major factor and is of great concern for crop production. Abiotic stresses, including salt and drought stress, restrain the crop yield. The aim of this particular study is to understand what role do plant growth-promoting rhizobacteria (PGPR) play in combating the salinity and drought stresses through modification of nutritional profile. In the current study, inoculated barley (Hordeum vulgare L.) plants were subjected to various stresses such as 200 mM and 1000 mM salinity stress as well as drought stress, and then their various parameters such as seed germination as well as shoot and root biomasses and photosynthetic activity were compared with non-treated stressed barley plants. Our data depicted an improvement or significant enhancement of these parameters in PGPR (Pseudomonas fluorescens SBW25 and Pseudomonas putida KT2440) applied barley plants. Furthermore, the particle-induced X-ray emission (PIXE) technique was used for the elemental analysis of PGPR-inoculated and non-inoculated plants under stress vs. no stress conditions. Our PIXE analysis of various macro- and micronutrients revealed an enhancement of Ca, Mg, K, P, S, Al, and Si uptake in PGPR-treated plants. PGPR applications depicted reduced Cl− contents in 200 mM salt-stressed barley roots (KT2440 = 7.7 mg/kg and SBW25 = 6.3 mg/kg) and stems (KT2440 = 406.4 mg/kg and SBW25 = 365.5 mg/kg) as compared to controls (roots = 8.9 and stems = 469.5), while they displayed a significant increase in the barley leaves (KT2440 = 405 mg/kg and SBW25 = 416.4 mg/kg) when compared to control (110.6 mg/kg) under the same stress condition. In 1000 mM salt stress, a significant reduction in the Cl− content was observed in PGPR-applied barley roots (KT2440 = 7.6 mg/kg), stems (KT2440 = 1205.8 mg/kg and SBW25 = 1008.3 mg/kg), and leaves (KT2440 = 967.8 mg/kg and SBW25 = 530.8 mg/kg) when compared to controls (roots = 15.2 mg/kg, stems = 1605.2 mg/kg, and leaves = 1165.2 mg/kg). On the other hand, a significant increase in the Cl− content was noticed in PGPR-applied barley roots (KT2440 = 29.5 mg/kg and SBW25 = 25.8 mg/kg), stems (KT2440 = 1023.8 mg/kg and SBW25 = 894.9 mg/kg), and leaves (KT2440 = 369.2 mg/kg and SBW25 = 409.8 mg/kg) when compared to controls (roots = 13.5 mg/kg, stems = 505.3 mg/kg, and leaves = 219.9 mg/kg) under drought stress condition. PGPR application was also found to be effective for enhancing the uptake of micronutrients (Mn, Fe, Co, Ni, Cu, and Zn) in barley plant parts under control and also under stressed conditions. Overall, our findings revealed an improvement in the uptake of macro- and micronutrients for the enhancement of salinity and drought stress tolerance. Conclusively, these PGPR species are an effective source of plant stress tolerance and elevated growth of barley and related plants under stress conditions.

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Section: Pixe (Particle-inducedx-ray Emission) Analysismentioning

confidence: 99%

Plant Growth-Promoting Rhizobacteria (PGPR) Reduce Adverse Effects of Salinity and Drought Stresses by Regulating Nutritional Profile of Barley

Zaib,

Zubair,

Abbas

et al. 2023

Applied and Environmental Soil Science

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“…The standardization of the PIXE analysis is very important for obtaining accurate results. [2,7] The PIXE is based on a two-step process, i.e., creation of electron vacancies in the innermost shell and filling of these vacancies by outer shell electrons, as shown in Fig. 1.…”

Section: Introductionmentioning

confidence: 99%

Standardization of proton-induced x-ray emission technique for analysis of thick samples

et al. 2015

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This paper describes the standardization of the proton-induced x-ray emission (PIXE) technique for finding the elemental composition of thick samples. For the standardization, three different samples of standard reference materials (SRMs) were analyzed using this technique and the data were compared with the already known data of these certified SRMs. These samples were selected in order to cover the maximum range of elements in the periodic table. Each sample was irradiated for three different values of collected beam charges at three different times. A proton beam of 2.57 MeV obtained using 5UDH-II Pelletron accelerator was used for excitation of x-rays from the sample. The acquired experimental data were analyzed using the GUPIXWIN software. The results show that the SRM data and the data obtained using the PIXE technique are in good agreement.

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“…Among non-destructive analytical techniques, Particle Induced X-ray Emission (PIXE) is a highly sensitive, multi-elemental analytical technique which is already proved in all prospective areas such as thin films, water, air, archaeological and biological samples etc. This technique works on the principle of creation of inner-shell vacancies in the target element and measurements of the x-rays emitted from the sample by using semiconductor detectors [1][2][3][4][5]. PIXE can only be used for detection of elements in the range because the energy of the characteristic x-rays emitted by low-Z elements is so low to be detected by presently available detection system.…”

Section: Introductionmentioning

confidence: 99%

An Elemental Analysis of Different CD-R discs

Pishkoo

2014

JAP

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Particle Induced X-ray Emission (PIXE): A Tool of Qualitative Elemental Analysis for Biological Sample

Cited by 3 publications

References 8 publications

Plant Growth-Promoting Rhizobacteria (PGPR) Reduce Adverse Effects of Salinity and Drought Stresses by Regulating Nutritional Profile of Barley

Plant Growth-Promoting Rhizobacteria (PGPR) Reduce Adverse Effects of Salinity and Drought Stresses by Regulating Nutritional Profile of Barley

Standardization of proton-induced x-ray emission technique for analysis of thick samples

An Elemental Analysis of Different CD-R discs

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