Particle Bombardment Mediated Transformation

Finer, John J.; Finer, Kim R.; Ponappa, Tilak

doi:10.1007/978-3-642-60234-4_3

Cited by 25 publications

(19 citation statements)

References 101 publications

(54 reference statements)

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“…Function of cytosine methylation and cosuppression mutually is consequential for down-regulation of transgene expression which is sometimes very incompatible and irregular (Komari et al 1998 ;Finer et al 1999 ;Iyer et al 2000 ;Dong and Li 2006 ) . Methylation of promoter sequences mainly cause the gene silencing at transcriptional level and in this manner chromatin re-modeling proteins are attracted to these sites by interfering with assembly of the transcription factors (Meyer 2000 ;Wang and Waterhouse 2002 ) .…”

Section: Transgene Expressionmentioning

confidence: 99%

“…Both the methods of transformation ( Agrobacterium /gene gun) are known for the stable integration and expression of the foreign genes into the plant cells (Briddon et al 1998 ;Finer et al 1999 ;Aragão 2002 ) . To observe the transient expression, tissues are bombarded/cocultivated and cultured for a speci fi c duration, generally 24-48 h. Then the tissues are evaluated for the expression of transgene.…”

Section: Transgene Expressionmentioning

confidence: 99%

“…After particle bombardment integration of exogenous DNA takes place into plant's genome in the form of simple, single copy or complex multiple copies insertions (Christou 1997 ;Finer et al 1999 ;Smith et al 2001 ) . This insertion of transgene process is considered to takes place in two-steps.…”

Section: Integration Of Transgenementioning

confidence: 99%

“…Regulatory sequences are modi fi ed by deletions or mutations and bombarded into plant cells. Control elements are identi fi ed by dissection and promoters are studied for up or down-regulation under that speci fi c physiological condition (Vain et al 1996 ;Komari et al 1998 ;Finer et al 1999 ) . Effectiveness of transgenes and promoters are estimated through transient expression assays before commencement of the plant genetic engineering (Jefferson 1987 ;de-la Riva et al 1998 ) .…”

Section: Transgene Expressionmentioning

confidence: 99%

See 3 more Smart Citations

Plant Genetic Engineering: Problems and Applications

Rashid

Husnain

Riazuddin

2012

Crop Production for Agricultural Improvement

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Section: Transgene Expressionmentioning

confidence: 99%

Section: Transgene Expressionmentioning

confidence: 99%

Section: Integration Of Transgenementioning

confidence: 99%

Section: Transgene Expressionmentioning

confidence: 99%

See 2 more Smart Citations

Plant Genetic Engineering: Problems and Applications

Rashid

Husnain

Riazuddin

2012

Crop Production for Agricultural Improvement

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“…Microprojectile bombardment has been used successfully to transform plant genomes since its development in the mid-1980s (Finer et al, 1999). In general, this method propels DNA-coated particles into plant cells, where the DNA integrates into the chromosome at one or more locations.…”

Section: Introductionmentioning

confidence: 99%

A transgenic locus in soybean exhibits a high level of recombination

Choffnes

Philip

Vodkin

2001

In Vitro Cell.Dev.Biol.-Plant

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We have examined transgene inheritance in over 300 progeny of a line of soybean (Glycine max ) transformed by particle bombardment with a construct containing bovine b-casein under the control of the soybean lectin 5 0 and 3 0 regulatory elements. Four copies of the casein transgene, located at a single locus, exhibit a high frequency of recombination that resulted in novel patterns in approximately 16% of the progeny in both the T 1 and T 2 generations. Characterization of the transgene locus using restriction enzymes that do not cut the transformation plasmid showed that all four transgene copies are at a single locus no larger than approximately 40 kb in size. Therefore, the recombination events resulting in the loss of transgene DNA are taking place within a limited physical distance on the host chromosome. This is the first report extensively documenting transgene instability at the DNA level in a plant transformed via particle bombardment. As this report indicates, a seemingly simple phenotype (presence of the foreign protein) may conceal inherent genetic instability at the DNA level.

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Levitation and movement of tripalmitin‐based cationic lipospheres on a dielectrophoresis‐based lab‐on‐a‐chip device

Fabbri

Borgatti

Manaresi³

et al. 2008

J of Applied Polymer Sci

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Dielectrophoresis (DEP) is a very valuable approach for designing and developing laboratory-on-achip (lab-on-a-chip) devices that are able to manipulate microparticles and cells. Lab-on-a-chip technology will enable laboratory testing to move from laboratories using complex equipment to nonlaboratory settings. We used a lab-on-a-chip device, the SmartSlide, which carries 193 parallel electrodes and generates up to 50 cylinder-shaped DEP cages able to entrap microparticles and cells within DEP cages and move them along the chip. For lab-on-a-chip technology, the characterization of microparticles exhibiting a differential ability to be DEP-caged, levitated, and moved is important for the development of both diagnostic and therapeutic protocols. We determined whether the SmartSlide could be used to levitate and move tripalmitin-based lipospheres carrying increasing concentrations of dihexadecyl dimethyl ammonium bromide (DHDAB) as a cationic surfactant. The data obtained with this DEP-based platform showed that DEP caging, levitation, and movement of the cationic lipospheres depended on the percentage of DHDAB. Tripalmitin lipospheres containing 6% DHDAB could be DEP-caged and manipulated. On the contrary, lipospheres containing 12% DHDAB did not exhibit an efficient ability to be DEP-caged and moved throughout the chip. To our knowledge, this is the first report on the possible use of a DEP-based lab-on-a-chip device for guided manipulation of lipospheres. This information might be of interest in the fields of drug discovery, delivery, and diagnosis.

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Particle Bombardment Mediated Transformation

Cited by 25 publications

References 101 publications

Plant Genetic Engineering: Problems and Applications

Plant Genetic Engineering: Problems and Applications

A transgenic locus in soybean exhibits a high level of recombination

Levitation and movement of tripalmitin‐based cationic lipospheres on a dielectrophoresis‐based lab‐on‐a‐chip device

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