Particle-based technologies for osteoarthritis detection and therapy

Kavanaugh, Taylor E.; Werfel, Thomas A.; Cho, Hongsik; Hasty, Karen A.; Duvall, Craig L.

doi:10.1007/s13346-015-0234-2

Cited by 63 publications

(46 citation statements)

References 130 publications

(126 reference statements)

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“…These results are promising for the development of other TF drugs. In addition, other approaches to target TFs that limit the adverse effects have also emerged, such as local injection or the use of nanodrug delivery systems ( [199,200]). In OA, specific joints are often involved, which makes it likely that local injection of TF drugs can be beneficial without many side effects.…”

Section: Transcription Factors As Therapeutic Targetsmentioning

confidence: 99%

Transcription Factors in Cartilage Homeostasis and Osteoarthritis

Neefjes

Caam

Kraan

2020

Biology

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Osteoarthritis (OA) is the most common degenerative joint disease, and it is characterized by articular cartilage loss. In part, OA is caused by aberrant anabolic and catabolic activities of the chondrocyte, the only cell type present in cartilage. These chondrocyte activities depend on the intra- and extracellular signals that the cell receives and integrates into gene expression. The key proteins for this integration are transcription factors. A large number of transcription factors exist, and a better understanding of the transcription factors activated by the various signaling pathways active during OA can help us to better understand the complex etiology of OA. In addition, establishing such a profile can help to stratify patients in different subtypes, which can be a very useful approach towards personalized therapy. In this review, we discuss crucial transcription factors for extracellular matrix metabolism, chondrocyte hypertrophy, chondrocyte senescence, and autophagy in chondrocytes. In addition, we discuss how insight into these factors can be used for treatment purposes.

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Section: Transcription Factors As Therapeutic Targetsmentioning

confidence: 99%

Transcription Factors in Cartilage Homeostasis and Osteoarthritis

Neefjes

Caam

Kraan

2020

Biology

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“…As the most common chronic joint disease, osteoarthritis (OA) is regarded as the primary cause of disability among older adults [1,2]. But research focusing on early diagnosis and treatment of OA is still at its infancy [3]. Therefore, developing highly specific theranostic methods is imperative.…”

Section: Introductionmentioning

confidence: 99%

MMP-13 Enzyme and pH Responsive Theranostic Nanoplatform for Osteoarthritis

Lan

Chen

Pang

et al. 2020

Preprint

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Stimulus-responsive therapy permits precise control of therapeutic effect only at lesion of interest, which determines it a promising method for diagnosis and imaging-guided precision therapy. The acid environment and overexpressed matrix metalloproteinases-13 (MMP-13) are typical markers in osteoarthritis (OA). However, stimulus-responsive therapeutic platform with high specificity for OA basing on the two markers has not been developed yet. We herein demonstrate a new stimuli-responsive theranostic strategy against OA based on a novel multifunctional nano-micelle which is rationally designed and yet readily synthesized. Such nanoplatform is incorporated with a motif specifically targeting on cartilage, a motif responsive to matrix metalloproteinases-13 to specifically report OA condition and biodynamics of nano-micelles, an anti-inflammatory drug (e.g., psoralidin (PSO)) from traditional Chinese medicine, and a biocompatible polymeric skeleton for sustainable drug release in response to the acidic OA condition. The high effectiveness of this targeted precision therapy is demonstrated comprehensively by both in vitro an in vivo evidences.

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“…The chemical composition of PLGA particles can be tailored such that a compound is released in vivo over the period of several weeks. PLGA has also been used in preclinical animal trials and human clinical therapies to locally administer compounds to various organs and tissues 15,16 . Other hydrophobic polymers can also be used in the generation of biodegradable microspheres.…”

Section: Discussionmentioning

confidence: 99%

Sustained Administration of β-cell Mitogens to Intact Mouse Islets <em>Ex Vivo</em> Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

Pasek

Kavanaugh

Duvall

et al. 2016

JoVE

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The development of biomaterials has significantly increased the potential for targeted drug delivery to a variety of cell and tissue types, including the pancreatic β-cells. In addition, biomaterial particles, hydrogels, and scaffolds also provide a unique opportunity to administer sustained, controllable drug delivery to β-cells in culture and in transplanted tissue models. These technologies allow the study of candidate β-cell proliferation factors using intact islets and a translationally relevant system. Moreover, determining the effectiveness and feasibility of candidate factors for stimulating β-cell proliferation in a culture system is critical before moving forward to in vivo models. Herein, we describe a method to co-culture intact mouse islets with biodegradable compound of interest-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres for the purpose of assessing the effects of sustained in situ release of mitogenic factors on β-cell proliferation. This technique describes in detail how to generate PLGA microspheres containing a desired cargo using commercially available reagents. Additionally, this method utilizes 96-well plates to minimize the amount of reagents necessary to assess β-cell proliferation. This protocol can be readily adapted to use alternative biomaterials and other endocrine cell characteristics such as cell survival and differentiation status.

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Particle-based technologies for osteoarthritis detection and therapy

Cited by 63 publications

References 130 publications

Transcription Factors in Cartilage Homeostasis and Osteoarthritis

Transcription Factors in Cartilage Homeostasis and Osteoarthritis

MMP-13 Enzyme and pH Responsive Theranostic Nanoplatform for Osteoarthritis

Sustained Administration of β-cell Mitogens to Intact Mouse Islets <em>Ex Vivo</em> Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

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Particle-based technologies for osteoarthritis detection and therapy

Cited by 63 publications

References 130 publications

Transcription Factors in Cartilage Homeostasis and Osteoarthritis

Transcription Factors in Cartilage Homeostasis and Osteoarthritis

MMP-13 Enzyme and pH Responsive Theranostic Nanoplatform for Osteoarthritis

Sustained Administration of &#946;-cell Mitogens to Intact Mouse Islets <em>Ex Vivo</em> Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

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Sustained Administration of β-cell Mitogens to Intact Mouse Islets <em>Ex Vivo</em> Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres