Particle and naked RNA mycoviruses in industrially cultivated mushroom Pleurotus ostreatus in China

β-Glucan Synthase Gene Overexpression and β-Glucans Overproduction in Pleurotus ostreatus Using Promoter Swapping

“…Protoplasts preparation and PEG/CaCl 2 -mediated transformation of P. ostreatus TD 300 were performed as described previously [20], [21]. The introduced foreign DNA was the homologous recombination fragment for swapping GLS promoter.…”

Immobilization of Mycelial Pellets from Liquid Spawn of Oyster Mushroom Based on Carrier Adsorption

“…LSM was composed of 10 g corn flour, 20 g soybean meal powder, 30 g sucrose, 5 g yeast extract, 1 g potassium dihydrogen phosphate (KH 2 PO 4 ), 0. tap water. CCM was a mixture of 100 g cottonseed hulls, 1 g lime, 1 g gypsum, and 110 mL tap water (Qiu et al, 2010). TD300 was stored on PDA slants at 4°C and was transferred every 2 months.…”

“…The mycelia were cultivated for 96 h, reaching the maximum biomass, and then harvested to prepare immobilized spawn. Conventional solid spawn was prepared as described (Qiu et al, 2010).…”

“…Experiments of basidiomata production were carried out using plastic bags containing 1000 g CCM. Five grams of immobilized spawn or solid spawn (as control) were inoculated into one end of a plastic bag and cultivated as described by Qiu et al (2010). Biological efficiency (BE) was defined as the percentage of the fresh weight of harvested mushrooms over the dry weight of inoculated substrates.…”

The PoV mycovirus affects extracellular enzyme expression and fruiting body yield in the oyster mushroom, Pleurotus ostreatus

“…In the edible mushroom Pleurotus ostreatus, mycoviral infections can cause symptoms such as irregular mycelial growth rate, decreased fruiting body yield, and malformed fruiting bodies, and thus occasionally affect cultivation of the mushroom. As mentioned above, several mushroom diseases have been reported to be associated with mycoviruses, such as oyster mushroom spherical virus (OMSV) 6 , oyster mushroom isomeric virus (OMIV) 7 , P. ostreatus spherical virus (POSV) 8 , oyster mushroom dsRNA virus (OMDV) 9 , P. ostreatus virus 1 (PoV1) 10 , and P. ostreatus ASI2792 virus (PoV-ASI2792; PoV) 3 .…”