Particle aggregation in photosynthetic membranes of the blue-green alga Anacystis nidulans

Verwer, W.; Ververgaert, P.H.J.Th.; Leunissen‐Bijvelt, J.; Verkleij, Arie J.

doi:10.1016/0005-2728(78)90021-x

Cited by 23 publications

(7 citation statements)

References 19 publications

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“…1, A and B) particle-free regions were seen in almost all of the PF of the cytoplasmic membrane. This is in agreement with previous reports by other investigators (1,2,4,16). At 13°C, the particle-free regions were small (Fig.…”

supporting

confidence: 94%

Chilling-Susceptibility of the Blue-Green Alga Anacystis nidulans

Ono¹,

Murata²

1982

Plant Physiol.

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The lipid phase of cytoplasmic membrane was studied by freeze-fracture electron microscopy in the chilling-susceptible blue-green alga, Anacystis nidulans. At growth temperatures, intramembrane particles were distributed at random in the fracture faces of cytoplasmic membrane, whereas, at chilling temperatures, the fracture faces were composed of particle-free and particle-containing regions. These findings indicate that lipids of the cytoplasmic membrane were in the liquid-crystalline state at the growth temperatures and in the phase-separation state at the chilling temperatures.

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supporting

confidence: 94%

Chilling-Susceptibility of the Blue-Green Alga Anacystis nidulans

Ono¹,

Murata²

1982

Plant Physiol.

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“…Further, the formation of gel-phase lipids was confirmed by X-ray diffraction measurements (26). These studies not only support the phase separation into coexisting domains in thylakoid membranes in line with that found in our simulations but also suggest the potential role of such domains in controlling the partitioning of integral membrane proteins (23)(24)(25)(26). It is worth mentioning the red alga G. Corticata studied here, abundantly grows in the Saurashtra coast of India during autumn-winter-spring when the seawater temperature ranges ~25-28 °C (58).…”

Section: Discussionsupporting

confidence: 84%

“…The scope for elucidation of phase properties and the structure of nanodomains in native thylakoid lipid membranes is very limited. However, of direct relevance to our finding, a previous study using freeze-fracture electron microscopy had captured the formation of gel phase lipid at 15 °C in the thylakoid membrane of thermophilic cyanobacteria that grow at 38 °C (23,25). In addition, this study also showed the presence of smooth particle-free patches in these membranes that signals the presence of phase-separated gel phase lipids from which intrinsic proteins are excluded.…”

Section: Discussionsupporting

confidence: 66%

“…There is a lack of consensus regarding the lateral heterogeneity in thylakoid membranes. The formation of domains has been reported and the different regions of these membranes are thought to have different lipid compositions (23)(24)(25)(26)(27). Whereas other studies suggest that the bulk lipids of thylakoid membranes do not display lateral heterogeneity (28).…”

Section: Introductionmentioning

confidence: 99%

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Phase transition in atomistic simulations of model membrane with thylakoid lipids of red algae

Rathod

Chavda

Manna

2022

Preprint

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Marine algae are diverse photosynthetic organisms, profoundly rich in bioactive compounds. Temperature is a major factor in algal cultivation and biomass production. At the cellular level, the change of temperature is reflected in oscillating algal lipid/fatty acid profile and inhibition of photosynthetic activities. The function of thylakoid membrane system is intimately dependent on its lipid matrix, however the molecular organization of these lipid membranes and particularly their adaptive arrangements under temperature stress remain largely unexplored. The present work employing extensive atomistic simulations provides the first atomistic view of the phase transition and domain coexistence in model membrane composed of thylakoid lipids of a marine alga, between 10-40 °C. The model membrane undergoes a transition from a gel-like phase at 10-15 °C to a homogeneous liquid-disordered phase at 40 °C. Clear evidences of spontaneous phase separation into coexisting nanoscale domains are detected at intermediate temperatures. Particularly at 25-30 °C, we identified the formation of a stable rippled phase, where the gel-like domains rich in saturated and nearly hexagonally packed lipids separated from fluid-like domains enriched in lipids containing polyunsaturated chains. Cholesterol impairs the phase transition and the emergence of domains, and induces a fairly uniform liquid-ordered phase in the membrane over the temperatures studied. The results have implications in understanding the role of lipids in temperature adaptation in algal.

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“…Thus, the verrucose and reticulate textured particle-free areas of the A. nidulans membranes may correspond to regions containing different kinds of lipids, or, as in the case of the lecithin bilayers, a possible different ordering of the lipids. Both lateral (15,16,18,21,27) and vertical (17,28) displacement of membrane particles have been proposed to explain the formation of particle-free areas in cooled membranes. FIG.…”

Section: Discussionmentioning

confidence: 99%

Lateral and vertical displacement of integral membrane proteins during lipid phase transition in Anacystis nidulans.

Armond¹,

Staehelin²

1979

Proc. Natl. Acad. Sci. U.S.A.

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Alterations in membrane structure as a result of lipid phase transitions have been studied in Anacystis nidulans, a blue-green alga. Cells grown at 38'C were subjected to temperature transitions of 380C -21'C and 380C -100C, previously shown to produce substantial changes in photosynthetic activities, and examined by freeze-fracture electron microscopy. As a result of these treatments, large particle-free regions appeared on the fracture faces of both the plasma and thylakoid membranes. Particle density measurements suggest that the displacement of the integral membrane protein complexes occurs in both lateral and vertical directions. Returning the cells to 380C resulted in the restoration of normal membrane morphology, indicating that the proteins were not lost from the membrane. Such displacement of the integral membrane protein complexes could contribute significantly to the temperature-dependent alterations in the functional activity of membrane-bound enzymatic complexes.The temperature dependency of the physical state of membranes has been extensively studied in model systems (1-9), intact cells (10-19), and isolated organellar and membrane fractions (10,(19)(20)(21). In these studies, it has been shown that lipid species and fatty acid composition have a direct effect upon the phase transition temperature of the membrane. It has also been demonstrated that alterations in the activation energy of the membrane-bound enzymatic systems are correlated with the membrane phase transitions (10,11,14,(19)(20)(21). Although the protein constituents of the membrane can affect the fluidity of membranes, they apparently play no role in determining the phase transition temperature (7). However, correlations between enzymatic function and the physical state of the membrane stress the fact that protein-lipid interactions are vital for the proper physiological functioning of the membrane.Previous studies of the blue-green alga Anacystis nidulans have shown that a correlation exists between the physical state of the lipids and such parameters as chlorophyll a fluorescence, P700 reduction, and photosynthetic oxygen evolution, parameters that involve integral membrane protein complexes (10). It was demonstrated that for cells grown at 380C, a change in slope in Arrhenius plots of such membrane functions occurred at approximately 220C to 240C. More recently, a second slope change has been detected at approximately 100C to 12'C (22). Linden et al. (14) have interpreted similar observations to indicate the onset (slope change at the higher temperature) and completion (slope change at the lower temperature) of the lateral phase separation of membrane lipids. Recent electron spin resonance experiments (23) have indicated that, in Anacystis lipids from cells grown at 380C, the onset of the lateral phase separation occurs at a temperature somewhat higher than 240C, but the experiments are in agreement with the completion of the phase transition at 100C. To learn more about the structural basis of the functional changes, we...

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Particle aggregation in photosynthetic membranes of the blue-green alga Anacystis nidulans

Cited by 23 publications

References 19 publications

Chilling-Susceptibility of the Blue-Green Alga Anacystis nidulans

Chilling-Susceptibility of the Blue-Green Alga Anacystis nidulans

Phase transition in atomistic simulations of model membrane with thylakoid lipids of red algae

Lateral and vertical displacement of integral membrane proteins during lipid phase transition in Anacystis nidulans.

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