Participation of prostaglandin D 2 in the mobilization of the nuclear-localized CTP:phosphocholine cytidylyltransferase alpha in renal epithelial cells

Favale, Nicolás Octavio; Pescio, Lucila Gisele; Santacreu, Bruno Jaime; Márquez, Marı́a Gabriela; Sterín-Speziale, Norma

doi:10.1016/j.bbalip.2016.03.025

Cited by 1 publication

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alternatively, propargyl-Cho-Alexa 488labeled cells were incubated with 3% BSA in PBS 0.1% (v/v) Triton X-100 at room temperature for 60 min, followed by incubation at 37°C for 60 min with 1:100 mouse anti-Lamin A/C antibody (Millipore) a kind gift from Dr. Nicolas Favale (Buenos Aires University, Argentina). Primary interactions were detected using a 1:500 F(ab´)2 fragment of goat anti-mouse IgG conjugated with Cy3 (Jackson ImmunoResearch) [28]. ER was labeled with 1:250 Alexa 647conjugated concanavalin A (Molecular Probes) kindly provided by Dr. Nicolas Favale (Buenos Aires University, Argentina) as described in [28].…”

Section: Subcellular Fractionationmentioning

confidence: 99%

“…Primary interactions were detected using a 1:500 F(ab´)2 fragment of goat anti-mouse IgG conjugated with Cy3 (Jackson ImmunoResearch) [28]. ER was labeled with 1:250 Alexa 647conjugated concanavalin A (Molecular Probes) kindly provided by Dr. Nicolas Favale (Buenos Aires University, Argentina) as described in [28]. Anti-CT α for immunofluorescence studies was a kind gift from Dr. Rosemary Cornell (Simon Fraser University, Canada).…”

Section: Subcellular Fractionationmentioning

confidence: 99%

See 1 more Smart Citation

De novo lipogenesis at the mitotic exit is used for nuclear envelope reassembly/expansion. Implications for combined chemotherapy

et al. 2019

View full text Add to dashboard Cite

Mitosis has been traditionally considered a metabolically inactive phase. We have previously shown, however, that extensive alterations in lipids occur as the cells traverse mitosis, including increased de novo fatty acid (FA) and phosphatidylcholine (PtdCho) synthesis and decreased lysophospholipid content. Given the diverse structural and functional properties of these lipids, we sought to study their metabolic fate and their importance for cell cycle completion. Here we show that FA and PtdCho synthesized at the mitotic exit are destined to the nuclear envelope. Importantly, FA and PtdCho synthesis, but not the decrease in lysophospholipid content, are necessary for cell cycle completion beyond G 2 /M. Moreover, the presence of alternative pathways for PtdCho synthesis renders the cells less sensitive to its inhibition than to the impairment of FA synthesis. FA synthesis, thus, represents a cell cycle-related metabolic vulnerability that could be exploited for combined chemotherapy. We explored the combination of fatty acid synthase (FASN) inhibition with agents that act at different phases of the cell cycle. Our results show that the effect of FASN inhibition may be enhanced under some drug combinations.

show abstract

Section: Subcellular Fractionationmentioning

confidence: 99%

Section: Subcellular Fractionationmentioning

confidence: 99%