Participation of Membrane Sulfhydryl Groups in the Epididymal Maturation of Human and Rabbit Spermatozoa

Reyes, Alberto; Mercado, Efraı́n; Goicoechea, Blanca; Rosado, Adolfo

doi:10.1016/s0015-0282(16)42263-6

Cited by 35 publications

(7 citation statements)

References 14 publications

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“…Various maleimides have been reported to have inhibitory effects on human and rabbit spermatozoa, possibly by a mechanism involving membrane sulphydryl groups Mercado, Carvajal, Reyes & Rosado, 1976;Reyes, Mercado, Goicoechea & Rosado, 1976).…”

Section: Discussionmentioning

confidence: 99%

Effect of antidepressant drugs on the in-vitro eggpenetrating ability of golden hamster epididymal spermatozoa

Stein¹,

Schnieden²

1983

Reproduction

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Tricyclic antidepressants appeared to be without effect, except for desipramine which significantly decreased whiplash motility after spermatozoa were added to eggs, and clomipramine which decreased motility and whiplash motility in epididymal sperm suspensions after pretreatment of males. Mianserin and viloxazine were also without effect, but nomifensine significantly decreased sperm motility and whiplash motility and inhibited egg penetration almost completely. After 3 h preincubation with 0\m=.\75mmol nomifensine hydrogen maleate/l, 2/181 and 0/256 eggs were penetrated in two separate series of experiments. Control groups in these series gave medians of 90\p=n-\100%penetration by 4\m=.\5\p=n-\5\m=.\5h after spermatozoa and eggs were mixed. Maleic acid had a similar effect (1/253 eggs penetrated) whilst nomifensine hydrochloride was inactive, suggesting that the effect was due to the maleate moiety of the original nomifensine hydrogen maleate salt used.

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Section: Discussionmentioning

confidence: 99%

Effect of antidepressant drugs on the in-vitro eggpenetrating ability of golden hamster epididymal spermatozoa

Stein¹,

Schnieden²

1983

Reproduction

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“…Maturation of mammalian spermatozoa in the epididymis is associated with an increase in disulfides in the nucleus, perinuclear materials, and in the tail components [Calvin and Bedford, 1971;Bedford and Calvin, 1974a, b]. The number of reactive membrane SH groups has been shown to reduce markedly during epididymal maturation and decrease slightly during in vitro "capacitation" [Reyes et al, 1976;Rosado et al, 19731. The relation of SH oxidation to capacitation and the acrosome reaction has not been studied up to now and the mechanism by which DTT prevents capacitation is at present entirely unknown.…”

Section: Discussionmentioning

confidence: 99%

Promotion of capacitation of guinea pig spermatozoa by the membrane mobility agent, A₂C, and inhibition by the disulfide‐reducing agent, DTT

1982

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The membrane mobility agent A2C accelerates the onset of the acrosome reaction of guinea pig spermatozoa by promoting capacitation. Spermatozoa incubated in a suspension of A2C particles in Ca2+‐free medium for one hour undergo a synchronous, rapid acrosome reaction upon the addition of Ca2+. These acrosome‐reacted spermatozoa are capable of fertilization as assessed by their ability to penetrate (fuse with) zona‐free hamster eggs. The disulfide‐reducing agent, dithiothreitol (DTT) inhibits A2C‐mediated capacitation. It also blocks fertilization of zone‐free eggs by acrosome‐reacted spermatozoa by preventing attachment of the spermatozoa to the egg plasma membrane. The mode of A2C action on spermatozoa is compared to that of A2C‐induced fusion in somatic cells. The similarity of the molecular events in the sperm membrane during capacitation and the acrosome reaction to these in other fusion events is pointed out. Inhibition of capacitation by DTT points to the importance of membrane and/or submembrane proteins and thiol groups in this process. Oxidation of sperm membrane SH groups may play an important role in in vivo capacitation.

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“…In addition to its effect of increasing structural stiffness of sperm flagella (Cornwall et al, 1988;Cornwall and Chang, 1990), diamide is known to decrease oxygen and Ca2+ uptake by mitochondria, an action that can be restored by dithioerythritol (Siliprandi et al, 1974). Other thiol-oxidising agents have also been shown to suppress Ca2+ uptake by bovine epididymal sperm (Breitbart and Lardy, 19871, oxygen uptake, as well as motility, of rabbit spermatozoa (Reyes et al, 1976) and motility of human sperm (Dawson et al, 1979). In contrast, reported effects of DTT on mammalian spermatozoa include an increase in sensitivity and response of demembranated sperm to localised stimulation by ATP (Yeung and Woolley, 1983), an increase in the percentage of demembranated sperm undergoing reactivation (Lindemann et al, 1988), and probably an increase in membrane stability as reflected by the inhibition of acrosome reaction and sperm-egg fusion induced by membrane mobility agents (Fleming et al, 1982).…”

Section: Influence Of Thiol-reducing or Oxidising Agents On Motility mentioning

confidence: 99%

“…both in the nucleus and the tail. Caput spermatozoa have more reactive SH groups than cauda spermatozoa in rats (Calvin and Bedford, 1971;Calvin et al, 1973;Shalgi et al, 1989), guinea pigs (Huang et al, 1984), and rabbits and men (Reyes et al, 1976), whereas the total amount of SH and S-S remains constant during maturation . In addition to the well-recognised role of oxidation of thiol to disulphide bonds in nuclear condensation of maturing spermatozoa, involvement of oxidation of flagellar structural SH to S-S in the development from nonprogressive to progressive sperm motility has also been postulated (see Bedford, 1975).…”

Section: Introductionmentioning

confidence: 99%

Maturation of hamster epididymal sperm motility and influence of the thiol status of hamster and rat spermatozoa on their motility patterns

Yeung¹,

Oberländer²,

Cooper³

1994

Molecular Reproduction Devel

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A method for objective quantification of hamster sperm movement parameters as an indicator of maturation along the epididymis was established using a computerised system. Analysis of spermatozoa released into medium from five epididymal regions showed that the most drastic increases in percentage motility and curvilinear velocity (VCL) occurred from the distal corpus to the beginning of the proximal cauda and in straight-line velocity (VSL) from the beginning to a more distal site within the proximal cauda region. Both high osmolarity (400 mOsm/kg) and the thiol-oxidising agent diamide (10 microM) increased flagellar straightness of distal corpus spermatozoa, but VSL was increased only with the latter. The thiol-reducing agent dithiothreitol (DTT, 1mM) stimulated and maintained percentage motility and velocities of spermatozoa from the caput, stimulated only percentage motility of distal corpus sperm, but decreased velocities of those from the proximal cauda in prolonged incubation. In rats, diamide increased path straightness but not velocities of caput spermatozoa and yet caused immotility within 15 min, whereas DTT prolonged the maintenance of in vitro motility. The slight increases in kinematic parameters in the presence of DTT were enhanced by a 2-min preincubation with diamide. The finding that the effects of DTT and diamide were not compensatory suggests that the influence of the SH/S-S status on sperm movement is multifaceted, with decreasing sensitivity to stimulation upon sperm maturation.

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Participation of Membrane Sulfhydryl Groups in the Epididymal Maturation of Human and Rabbit Spermatozoa

Cited by 35 publications

References 14 publications

Effect of antidepressant drugs on the in-vitro eggpenetrating ability of golden hamster epididymal spermatozoa

Effect of antidepressant drugs on the in-vitro eggpenetrating ability of golden hamster epididymal spermatozoa

Promotion of capacitation of guinea pig spermatozoa by the membrane mobility agent, A₂C, and inhibition by the disulfide‐reducing agent, DTT

Maturation of hamster epididymal sperm motility and influence of the thiol status of hamster and rat spermatozoa on their motility patterns

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Participation of Membrane Sulfhydryl Groups in the Epididymal Maturation of Human and Rabbit Spermatozoa

Cited by 35 publications

References 14 publications

Effect of antidepressant drugs on the in-vitro eggpenetrating ability of golden hamster epididymal spermatozoa

Effect of antidepressant drugs on the in-vitro eggpenetrating ability of golden hamster epididymal spermatozoa

Promotion of capacitation of guinea pig spermatozoa by the membrane mobility agent, A2C, and inhibition by the disulfide‐reducing agent, DTT

Maturation of hamster epididymal sperm motility and influence of the thiol status of hamster and rat spermatozoa on their motility patterns

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Promotion of capacitation of guinea pig spermatozoa by the membrane mobility agent, A₂C, and inhibition by the disulfide‐reducing agent, DTT