Participation of cytophilic antibody in enhancement of interferon production in macrophages by under-neutralized NDV

Abstract

SummaryEnhancement of interferon production in mouse peritoneal macrophages by Newcastle disease virus (NDV) under-neutralized with whole antiserum or its IgG fraction was inhibited in cells pretreated with iodoacetamide, sodium nitrite or formaldehyde, which blocked adsorption of cytophilic antibody to macrophages. Mouse embryo primary culture cells had no receptor for eytophilic antibody, and in such a cell culture, under-neutralized NDV did not enhance interferon production. Antiserum enhanced interferon production by NDV which had adsorbed to cell surfaces, but did not affect NDV that had penetrated the cells.

Viral Factors Required for Interferon Induction by Newcastle Disease Virus in Mouse Macrophages and Chicken Embryo Cells

“…In fact, hydroxylamine-treated virus did contain some residual infectious NDV. However, according to an earlier report (Azuma, 1973) macrophages inoculated with infective NDV at a multiplicity of less than 5 p.f.u./cell, fail to produce interferon. These findings suggest that some component or factor of the hydroxylamine-inactivated NDV was actually responsible for interferon induction.…”

Effect of Mycobacterium tuberculosis BCG infection on the resistance of mice to ectromelia virus infection: participation of interferon in enhanced resistance

“…Three weeks later, these mice were used in the experiment. The preparation and assay of interferon were carried out as described previously (1). Normal and BCG-infected mice were inoculated intraperitoneally with 0.2 ml of 10-folddiluted EV, and subsequent deaths were recorded.…”