Participation of CXCL1 in the glial cells during neuropathic pain

Moraes, Thamyris Reis; Elisei, Lívia; Malta, Iago Henrique Silva; Galdino, Giovane

doi:10.1016/j.ejphar.2020.173039

Cited by 18 publications

(18 citation statements)

References 52 publications

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“…Intrathecal administration of CXCL1, 2 or 3 produced hyperalgesia in mice [ 52 ]. These chemokines have also been implicated in neuropathic pain [ 52 – 54 ] and nociceptive sensitization after traumatic brain injury [ 55 , 56 ]. CXCL1 in the synovial fluid of osteoarthritis patients correlated with the severity of pain [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of disease- and headache-specific mediators and pathways in migraine using blood transcriptomic and metabolomic analysis

et al. 2021

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Background Recent data suggest that gene expression profiles of peripheral white blood cells can reflect changes in the brain. We aimed to analyze the transcriptome of peripheral blood mononuclear cells (PBMC) and changes of plasma metabolite levels of migraineurs in a self-controlled manner during and between attacks. Methods Twenty-four patients with migraine were recruited and blood samples were collected in a headache-free (interictal) period and during headache (ictal) to investigate disease- and headache-specific alterations. Control samples were collected from 13 age- and sex-matched healthy volunteers. RNA was isolated from PBMCs and single-end 75 bp RNA sequencing was performed using Illumina NextSeq 550 instrument followed by gene-level differential expression analysis. Functional analysis was carried out on information related to the role of genes, such as signaling pathways and biological processes. Plasma metabolomic measurement was performed with the Biocrates MxP Quant 500 Kit. Results We identified 144 differentially-expressed genes in PBMCs between headache and headache-free samples and 163 between symptom-free patients and controls. Network analysis revealed that enriched pathways included inflammation, cytokine activity and mitochondrial dysfunction in both headache and headache-free samples compared to controls. Plasma lactate, succinate and methionine sulfoxide levels were higher in migraineurs while spermine, spermidine and aconitate were decreased during attacks. Conclusions It is concluded that enhanced inflammatory and immune cell activity, and oxidative stress can play a role in migraine susceptibility and headache generation.

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Section: Discussionmentioning

confidence: 99%

Identification of disease- and headache-specific mediators and pathways in migraine using blood transcriptomic and metabolomic analysis

et al. 2021

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“…CXCL1 and CXCR2 have been increasingly recognised to participate in the development and maintenance of neuropathic pain in both the CNS and PNS. 12,13,38 Mounting evidence suggests that CXCL1 is expressed in astrocytes and CXCR2 is distributed in neurons in the spinal cord. 30,38 Moreover, CXCL1 and CXCR2 were shown to be colocalised with CGRP, isolectin B4 (IB4) and NF200 in the DRG, indicating that CXCL1 and CXCR2 were expressed in neurons.…”

Section: Discussionmentioning

confidence: 99%

“…10,11 In the central nervous system (CNS), CXCR2 and CXCL1 are upregulated in different chronic pain models, including chronic constriction injury (CCI) of the sciatic nerve, spinal nerve ligation (SNL) and traumatic brain injury (TBI). [12][13][14] Studies on CXCR2 in the peripheral nervous system (PNS) have mainly focused on the dorsal root ganglion (DRG). CXCL1 and CXCR2 have been reported to play a role in neuropathic pain induced by CCI of the sciatic nerve.…”

Section: Introductionmentioning

confidence: 99%

C‐X‐C motif chemokine ligand 1 and its receptor C‐X‐C motif chemokine receptor 2 in trigeminal ganglion contribute to nerve injury‐induced orofacial mechanical allodynia

Yang

Liu

Zhang

et al. 2021

J of Oral Rehabilitation

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Background: Orofacial ectopic pain induced by trigeminal nerve injury is a serious complication of dental treatment. C-X-C motif chemokine ligand 1 (CXCL1) and its primary receptor C-X-C motif chemokine receptor 2 (CXCR2) contribute to the development and maintenance of neuropathic pain in the spinal nervous system, but their roles in trigeminal neuropathic sensation are still poorly understood.Objectives: This study aimed to investigate the exact role of CXCL1 and CXCR2 in the regulation of orofacial ectopic mechanical allodynia and their potential downstream mechanisms in the trigeminal ganglion (TG).

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“…Pre-injection of CCR2 antagonist into the intrathecal not only can prevent and relieve nerve pain but also can inhibit the activation of microglia and p38-MAPK [25][26][27] In addition, studies have shown that in neuropathic pain caused by CCI surgery, the expression of CXC motif chemokine ligand 1 (CXCL1) and its receptor CXCR2 in spinal dorsal horn neurons and DRG are increased, taking CXCR2 antagonists can reverse mechanical allodynia [28]. Further studies have found that CXCR2 and the activation of microglia may be involved in neuropathic pain, and p-p38 and p38-MAPK may be involved in it [29]. We also found that the levels of CCL2, CCR2, CXCL1 and CXCR2 in the spinal cord tissue of the model group increased in this study, and their expression decreased signi cantly after THP treatment.…”

Section: Discussionmentioning

confidence: 99%

Tetrahydropalmatinee alleviates diabetic neuropathic pain by inhibiting the inflammation via p38-MAPK signaling pathway in microglia

Cheng

Jiang

et al. 2021

Preprint

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Object: Exploring the effect of Tetrahydropalmatine (THP) on diabetic neuropathic pain (DNP) and its possible mechanism. Methods: The type 2 diabetic (T2DM) rat models were prepared by high-fat and high-sugar feeding combined with a single small-dose intraperitoneal injection of streptozotocin (STZ). When the mechanical withdrawal threshold (MWT) and the thermal withdrawal latency (TWL) of T2DM model rats decreased to less than 85% which were judged as DNP-bearing rats. After treatment with or without THP, the protein expression of hypertonic glycerol reactive kinase (p38), phosphorylated hypertonic glycerol-responsive kinase (p-p38) and OX42 (a specific marker of microglia) were detected by Western Blot and and the mRNA content of p38 and OX42 were detected by qRT-PCR. The expression of pro-inflammatory factors IL-1β, IL-6, TNF-α, as well as chemotactic factors and their receptors including CXCL1, CXCR2, CCL2 and CCR2 in spinal tissues were detected by ELISA. Serum FINS and GSP content were also detected by ELISA. Double-label immunofluorescence were used to observe the expression of OX42 and p-p38 in the spinal dorsal horn. Results: Results showed that THP inhibited microglial activation of spinal in DNP rats. And after THP intervention, the MWT and TWL of DNP rats decreased, the expression of p38, p-p38 and OX42 in the spinal cord tissues of rats was significantly reduced while the mRNA of p38 and OX42 also reduced. The expression of IL-1β, IL-6, TNF-α, CXCL1, CXCR2, CCL2 and CCR2 in the spinal cord tissues of rats was significantly reduced (P < 0.01). At the same time, THP significantly proved FINS, but did not affect FBG and GSP in DNP rats. Conclusions: THP significantly alleviates pain symptoms in DNP rats, and this effect may be achieved by inhibiting the inflammatory response caused by the activation of microglia mediated by the p38-MAPK signaling pathway.

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Participation of CXCL1 in the glial cells during neuropathic pain

Cited by 18 publications

References 52 publications

Identification of disease- and headache-specific mediators and pathways in migraine using blood transcriptomic and metabolomic analysis

Identification of disease- and headache-specific mediators and pathways in migraine using blood transcriptomic and metabolomic analysis

C‐X‐C motif chemokine ligand 1 and its receptor C‐X‐C motif chemokine receptor 2 in trigeminal ganglion contribute to nerve injury‐induced orofacial mechanical allodynia

Tetrahydropalmatinee alleviates diabetic neuropathic pain by inhibiting the inflammation via p38-MAPK signaling pathway in microglia

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