Partially Purified Reindeer (Rangifer Tarandus) Bone Morphogenetic Protein Has a High Bone-Forming Activity Compared With Some Other Artiodactyls

Jortikka, L.; Marttinen, A.; Ts, Lindholm

doi:10.1097/00003086-199312000-00008

Cited by 26 publications

(22 citation statements)

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“…After redissolving in 4 M GuHCl solution, the waterinsoluble material was dialyzed against 0.25 M citrate buffer, pH 3.1. The citrate-buffer-insoluble material was washed with deionized water and lyophilized (Jortikka et al 1993a).…”

Section: Bmp Materialsmentioning

confidence: 99%

“…Bone morphogenetic proteins (BMPs) stimulate regeneration of bone and cartilage (Jortikka et al 1993a,b, Cook 1999, Govender et al 2002, Wozney 2002, Cheng et al 2003, Pekkarinen et al 2003. The bone-healing properties of different recombinant and extracted BMPs have been studied using long bone defect models in rabbits (Hollinger et al 1998, Kokubo et al 2003, dogs Johnson 2000, Tuominen et al 2000), rats (Takagi and Urist 1982, Chen et al 2002, Matsuo et al 2003 and sheep (Gao et al , 1997.…”

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confidence: 99%

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Reindeer BMP extract in the healing of critical-size bone defects in the radius of the rabbit

Pekkarinen¹,

Jämsä

Määttä

et al. 2006

Acta Orthopaedica

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Background Native BMP extracts from reindeer effectively induce ectopic new bone formation in vivo, but their bone healing properties have not yet been evaluated. We investigated the effect of reindeer BMP extracts on the healing of long bone defects.Methods The implants tested contained 5 mg or 10 mg of unsterilized BMP extract from reindeer and 10 mg of gamma-sterilized BMP extract administered with collagen carrier (Lyostypt, B. Braun, Germany). 70 µg of rhBMP-2 with collagen carrier (InductOs; Wyeth Europa) served as positive control, and collagen implants (Lyostypt) and untreated defects served as negative controls. New Zealand White rabbits with 1.5 cm of critical-size radius bone defects were used, with 8 weeks of follow-up.Results Radiographic analysis showed bone formation (BF) to be higher in all groups containing BMPs than in the untreated controls. BF was also higher in the rhBMP-2 group, and marginally higher in the group treated with 10 mg of unsterilized reindeer BMP extract (p = 0.06) as compared to the collagen controls. Bone union (BU) was better in the unsterilized BMP extract groups and rhBMP-2 group than in the untreated controls. BU was also better in the implants with 10 mg of unsterilized reindeer BMP extract and rhBMP-2 than in the collagen-treated implants. The mean area of new bone at the site of the defect proved to be higher in all implants containing BMP than in the untreated defects. It was also higher in the groups with 10 mg of unsterilized reindeer BMP extract and rhBMP-2 than in the collagen-treated controls. Mechanical tests showed torsional stiffness of the bones to be higher in the group with 10 mg of unsterilized BMP extract than in the collagen

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Section: Bmp Materialsmentioning

confidence: 99%

mentioning

confidence: 99%

Reindeer BMP extract in the healing of critical-size bone defects in the radius of the rabbit

Pekkarinen¹,

Jämsä

Määttä

et al. 2006

Acta Orthopaedica

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“…The native reindeer bone extract has proved to possess better osteoinductivity than bovine or ostrich bone extracts, and it has been suggested that this is due to the fact that reindeers renew their antlers annually [7,8]. Furthermore, it has been suggested that more of the protein material extracted from the reindeer bone is in monocomponent form compared to that from other species such as cattle, sheep or pigs [7].…”

Section: Introductionmentioning

confidence: 99%

Reindeer bone extract can heal the critical-size rat femur defect

Tölli

Kujala

Jämsä

et al. 2010

International Orthopaedics (SICOT)

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Bone extract from reindeer induces new ectopic bone formation (BF) in muscle pouches, but its feasibility in experimental bone lesions has not been evaluated. We investigated the effects of implants, containing 2, 5, 15, 20 or 50 mg of reindeer bone extract in a collagen carrier, on the healing of 8-mm femur defects in 78 rats. We used 30 µg of recombinant human bone morphogenetic protein-2 (rhBMP-2) in a collagen carrier, collagen and untreated defects as controls. Bone healing was evaluated with radiographs, peripheral quantitative computed tomography (pQCT), biomechanics and histology. In comparison with empty defects, the groups receiving bone extracts showed more BF at three weeks and had better bone union (BU), larger mean cross-sectional bone area at the defect site in groups receiving higher doses of extract, showed greater torsional stiffness of the bones and higher maximum breaking load of bones at six weeks. In comparison to all other groups, in the rhBMP-2 group, BF and BU were best at the three-and six-week follow-up, bone area was largest and mechanical test results were best. Although rhBMP-2 is superior for new bone regeneration, native reindeer bone extract is also effective in the six-week follow-up period.

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“…The ability of BMPs to induce new bone and to heal bone defects has been well established in many experimental animal and clinical studies [7,8,9,11,12,13,14,21,22,24]. The sterilization of BMP before preclinical and clinical application is mandatory but problematic because BMPs do not tolerate heat management.…”

Section: Introductionmentioning

confidence: 99%

Influence of ethylene oxide sterilization on the activity of native reindeer bone morphogenetic protein

Pekkarinen

Hietala

et al. 2004

International Orthopaedics

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Partially Purified Reindeer (Rangifer Tarandus) Bone Morphogenetic Protein Has a High Bone-Forming Activity Compared With Some Other Artiodactyls

Cited by 26 publications

References 0 publications

Reindeer BMP extract in the healing of critical-size bone defects in the radius of the rabbit

Reindeer BMP extract in the healing of critical-size bone defects in the radius of the rabbit

Reindeer bone extract can heal the critical-size rat femur defect

Influence of ethylene oxide sterilization on the activity of native reindeer bone morphogenetic protein

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