This study includes case series of three cases of transverse vaginal septum with vaginal atresia. Cases presented with either primary amenorrhea or hypomenorrhea or secondary amenorrhea with complaints of cyclical abdominal pain. On examination, all cases had well developed secondary sexual characters, blind vaginal pouch with enlarged uterus felt on per rectal examination. Hormonal profile of all three cases was within normal limit. Karyotyping was done for all three cases, results were 46 XX. Radiological findings were that all cases had hematocolpos and one case had OHVIRA syndrome i.e.; absent right kidney with didelphys uterus with right hemivagina transverse septum. Management of all three cases included resection of transverse vaginal septum with vaginoplasty. Transverse vaginal septum was dissected and was used to create neovagina. Post-operatively, patients were advised to use phantom dilator, for 3-4 times in a day for atleast 3 months, to avoid vaginal stenosis. Cases were followed in OPD every monthly for 3 months, dysmenorrhea was relieved and patients were menstruating regularly with moderate flow for 3-4 days.