Partial success of marker-assisted selection of ‘A’ and ‘B’ onion lines in Brazilian germplasm

Ferreira, Roberta Rocha; Santos, C. A. F.

doi:10.1016/j.scienta.2018.08.002

Cited by 15 publications

(14 citation statements)

References 25 publications

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“…Our findings of male‐sterile and ‐fertile pollen based on anther colour and shape in male‐sterile plants are in accordance with the findings of Pathak (1994) and Santos, Leite, Oliveira, and Rodrigues (2010), who identified the maintainer and male‐sterile onion lines within the Brazilian population while monitoring S‐, T‐ and N‐cytoplasms, using 5' cob ‐marker and orf A50. Similarly, Ferreira and Santos (2018) identified male‐sterile and maintainer lines within the Brazilian onion population using orf‐ 725 marker and the AcSKP1 marker system.…”

Section: Discussionmentioning

confidence: 96%

Identification and characterization of important sterile and maintainer lines from various genotypes for advanced breeding programmes of onion (Allium cepa)

et al. 2020

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Onion is one of the major vegetable crops in terms of production as well as consumption. In the current research, available onion genetic stock was evaluated to identify male‐sterile lines and produce high‐yielding F1 hybrids for future breeding programmes. A mitochondrial DNA‐based marker was mapped and correlated with phenotypic traits to isolate male‐sterile plants. Based on the floral and pollen structure, nine putative male‐sterile lines were identified. On the other hand, for nuclear marker identification at Ms locus, two sets of primers were used, one for Ms dominant allele and another for sterile and maintainer plants. Results revealed that 70% of open pollinated varieties (OPVs) possess plants with sterile cytoplasm coupled with genetic sterility at Ms locus, called sterile “A” line. Approximately 20% of plants in some genotypes were identified with normal (N) cytoplasm having recessive fertility gene at Ms locus, called maintainer “B” line. Based on the present findings, “A”, “B” and “R” (restorer line), future F1 hybrid seed production systems in onion is discussed.

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Section: Discussionmentioning

confidence: 96%

Identification and characterization of important sterile and maintainer lines from various genotypes for advanced breeding programmes of onion (Allium cepa)

et al. 2020

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“…A wellcharacterized male sterile cytoplasm which prevents pollen development is available in Petunia (Gillman et al, 2009) and can be transferred by backcross to elite female lines in a breeding program. In this context, molecular markers associated to cytoplasm types are of value to trace sterile cytoplasms during their introgression into elite lines and to determine genetic purity of F1s (Shu et al, 2016;Swamy et al, 2017;Ferreira and Santos, 2018).…”

Section: Discussionmentioning

confidence: 99%

PCR identification of Petunia male sterile cytoplasm

Colombo

Hagiwara²

2019

Ornam. Hortic.

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Petunia is a very important ornamental plant with a broad range of flower colour and size, and most of the cultivars grown are propagated through seeds. Cytoplasmic male sterility (CMS) is a maternally inherited character determined by mitochondrial genes that results in impaired pollen development. The unique and well characterized male sterile cytoplasm in Petunia is a valuable resource for hybrids production because it prevents self-fertilization of mother plants and ensures the purity of F1s. Introgression of the male sterile cytoplasm in elite lines of Petunia is achieved following a backcross scheme and can be assisted using molecular markers associated to the trait of interest. The objective of this study was to develop a molecular marker to identify the male sterile cytoplasm of Petunia. A PCR-based marker amplifying a region of the mitochondrial CMS-associated urfS only in the male sterile plants was designed. Results showed differential PCR amplification of a ≈ 600 bp product in plants carrying male sterile cytoplasm in four Petunia species and their F1s and BC1 generations. A multiplex PCR reaction was subsequently set up, adding specific primers amplifying a ≈ 800 bp product from the conserved region trnT-trn-L of the chloroplast genome as a positive control in order to unambiguously identify the cytoplasm types as normal or sterile. A rapid, simple and precise molecular marker is now available for assisting breeding of F1 hybrids in Petunia.

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“…Leaf samples from seven plants of each progeny and parent were collected 30-40 days after sowing to constitute a bulk for total DNA extraction. The protocol for DNA extraction was the 2x CTAB method modified to 10,500 and 12,500 rpm in the first and second centrifugations, respectively, with 2% beta-mercaptoethanol incubation at 60ºC for 30 minutes for all samples (Ferreira & Santos, 2018). The DNA concentration extracted from each sample was determined by direct reading with a Multiskan Spectrophotometer (Thermo Scientific) at absorbance ratios of 260/280 nm.…”

Section: Plant Materials and Dna Extractionmentioning

confidence: 99%

Outcrossing and heterozygosity rates in tropical onion populations

Santos

Alves

2022

Com. Sci.

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Outcrossing and heterogosity rates are important for defining genetic conservation and breeding strategies, and these estimates are quite rare to onion. The aim of this study was to estimate cross-pollination and heterozygosity rates in tropical onion populations to guide development of new varieties. Eight tropical onion pairs were field isolated to be open pollination by insects. Parents vernalized bulbs were used for pair crossing, in alternate rows, 1:1. Seeds were harvested from the female parent to assess the progenies outcrossing and heterogigosity rates based on color of the red bulb, partially dominant over yellow, and four microsatellite loci. The outcrossing rates estimated with morphological marker, in the ‘BRS Rio Vale’ × ‘BRS Carrancas’, ‘Botucatu’ × ‘BRS Carrancas’, ‘IPA 11’ × ‘IPA 10’ and ‘BRS Alfa São Francisco’ × ‘IPA10’ crosses, ranged from 15% to 39%, with an average of 28.2%, while with microsatellite loci, in the ‘BRS Alfa São Francisco’×’IPA 11’, ‘Alfa SF RT’ × ‘BRS A. São Francisco’, ‘BRS Rio Vale’ × ‘Botucatu’ and ‘Cascuda T6’ × ‘Botucatu’ crosses, ranged from 33% to 71%, with an average of 42.7%, indicating a mixture of cross-pollination and self-pollination within progenies. The average values of heterozygosity in the eight populations ranged from 0.82 to 1.0, indicating the possibility of developing open pollinated onion varieties with greater adaptation to biotic and abiotic stresses.

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Partial success of marker-assisted selection of ‘A’ and ‘B’ onion lines in Brazilian germplasm

Cited by 15 publications

References 25 publications

Identification and characterization of important sterile and maintainer lines from various genotypes for advanced breeding programmes of onion (Allium cepa)

Identification and characterization of important sterile and maintainer lines from various genotypes for advanced breeding programmes of onion (Allium cepa)

PCR identification of Petunia male sterile cytoplasm

Outcrossing and heterozygosity rates in tropical onion populations

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