his study was contacted to investigate the effects of row spacing and different rate of mineral nitrogen fertilization with / or without Bio-fertilizer on fodder beet productivity, quality and soil properties. We evaluated the effects of N level placement at rate of 60, 90, 120 kg/fed with and without bio-fertilizer and row spacing. The treatments content : A1: 58 cm, A2: 76 cm, T1: 60 kg/ fed N. without bio-fertilizer, T2: 60kg/ fed N with bio-fertilizer, T3: 90 kg/ fed N. without bio-fertilizer, T4: 90 kg/ fed N with bio-fertilizer, T5: 120 kg/ fed N without bio-fertilizer and T6: 120 kg/ fed N with bio-fertilizer. The results showed that the nitrogen application on number of leaves and root diameter value were nonsignificantly increased 47.38 and 14.43 cm for T6, while the root length value 53.25 cm for T4 was significantly increased. The highest values of fresh and dry leaves in T4 were 6.90 and 0.90 ton/fed as effected by applying nitrogen fertilizer at rate of 90 kg N/fed with bio-fertilizer under 76 cm row spacing, while the highest values of fresh and dry root yield in T1 were 71.93 and 10.51 ton/fed, respectively. We may conclude that using as soil application 90 kg N fed -1 inter row spacing 58 cm could led to improving soil chemical properties, which in turn led to producing the highest root and leaves of fodder beet yield/ fed. The highest crude protein and fiber observed with leaves and roots under density row in (A2) 76 cm spaces compared with spacing raw 58 cm(A1), while NFE% content had a significant decrease with applying nitrogen fertilizer alone or combined with biofertilizer with leaves and roots under (A2) the values were 49.57, 68.21 compared with (A1). The T6 (120 kg/fed N + bio-fertilizer) recorded the highest values in crude protein while were 14.99 and 12.91 in laves and roots, respectively, while the highest value in crude fiber was obtained from 60 kg fed -1 nitrogen treatment without bio-fertilizer (T1), where values were 25.23 and 8.01%, respectively. In addition, the values of NFE% decrease significantly in T6. The values were increased significantly for IV-DMD%, GP (ml/200mgDM), IV-DOM% and ME (MJ/kg DM) under (A2) 76 cm spaces between rows compared with spacing raw 58 cm (A1) in leaves. While the roots were increased in GP, IV-DOM% and ME (MJ /kg DM) from 29.04 (ml/200mgDM), 77.33% and10.20(MJ/kg DM) spacing raw 58 cm (A1) to 31.95 (ml/200mgDM), 81.79% and 11.02(MJ/kg DM) density of row in (A2) 76 cm spaces, respectively. The treatment T6 recorded the highest value in leaves. while in the root the highest value recorded on treatment T5 in GP, IV-DOM% and ME (MJ/kg DM) analysis, where the values are 42.12 (ml/200mg DM) ,86.92% and 11.95 (MJ /kg DM), respectively except IV-DMD% were recorded high value 63.37% were recorded with T1. and GPSF with T2. These significantly increase may be due to short chain fatty acid (SCFA mmol/ml gas), Gas production structure fractions (GPSF ml/g DM), Gas production non structure fractions (GPNSF ml/g DM) and Microbial protein (MP g/kg DOM) in (A2) 7...