Partial sequence of the gene for a serine protease from a halophilic archaeumhaloferax mediterranei R4, and nucleotide sequences of 16S rRNA encoding genes from several halophilic archaea

Abstract

A part of the gene coding for a halophilic serine protease from a halophilic archaeum Haloferax mediterranei R4 was amplified by PCR and its 672 nucleotide sequence was determined. Tentative translation to the amino acid sequence suggested that the enzyme was quite similar to halolysin produced by another halophilic archaeum strain 172P1. Nucleotide sequences of 16S rRNA encoding genes from 9 halophilic archaea were determined. Alignment of 19 sequences known so far showed that there are more than 20 positions carrying bases or deletions specific for each halobacterial genus: Halobacterium, Haloarcula, Haloferax, and Halococcus.

Detection and preliminary characterization of extracellular proteolytic activities of the haloalkaliphilic archaeon Natronococcus occultus

“…All the gelatinolytic species were similarly affected by both inhibitors (not shown). Our results indicate that -at for all the extracellular proteases described in halophilic archaea (Izotova et al 1983;Kamekura and Seno 1990;Schmitt et al 1990;Stepanov et al 1992;Kamekura and Seno 1993) the extracellular proteases from N. occultus are of the serine type.…”

“…Many strains secrete caseinolytic and gelatinolytic activities into the medium (Gibbons 1957), but at present only a few proteolytic enzymes have been described and characterized. These are extracellular proteases of the serine type, which require high salt concentrations for activity and stability and are autolysed after dialysis against low-ionic-strength buffers (Izotova et al 1983;Kamekura and Seno 1990;Schmitt et al 1990;Stepanov et al 1992;Kamekura and Seno 1993).…”

Proposal to transfer Halococcus turkmenicus, Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena turkmenica gen. nov., comb. nov.

“…Halobacterium trapanicum JCM 9743 and strain GSL-11 have been shown to contain very similar 16s rRNA gene sequences (Kamekura & Seno, 1993) and exactly the same polar lipid pattern (Kamekura & DyallSmith, 1995). Halococcus turkmenicus, on the other hand, had a different glycolipid, S2-DGD, which is the characteristic lipid of Natrialba asiatica.…”

Isolation of halophilic and halotolerant bacteria from a Japanese salt field and comparison of the partial 16S rRNA gene sequence of an extremely halophilic isolate with those of other extreme halophiles

“…[The strain has been previously identified, (Takashina et al, 1990) and descriptions of the strain's cell shape and cell surface have also been previously reported (Hamamoto et al, 1988;Horikoshi et al, 1993).] We sequenced the TR-1 16S rRNA gene, using a cloned gene to avoid difficulties arising from multiple 16S rRNA genes which some halophilic archae are known to contain (Kamekura and Seno, 1993). The TR-1 16S rRNA sequence has high sequence similarity (>94.9%) with 16S rRNA sequences of Haloarcula species, which is consistent with our previous findings that TR-1 is a species belonging to the Haloarcula group (Takashina, et al, 1990).…”

“…5). Figure 5 also shows the alignment of the partial 16S rRNA sequence with other published sequence of Haloarcula species (Kamekura and Seno, 1993). Sequence similarity of the 16S rRNA genes of Ha.…”