Partial Purification of an Opiate Receptor from Mouse Brain

Lowney, Louise I.; Schulz, Karin; Lowery, Patricia J.; Goldstein, Avram

doi:10.1126/science.183.4126.749

Cited by 90 publications

(11 citation statements)

References 23 publications

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“…Similar effects, however, were observed when negatively charged lipids were used instead of the complete proteolipids (106). In view of the remarkable properti e s of the proteolipids and associ ated lipids fr om excitable tissues, in particular their high affinity, and in some cases stereospecifi c binding characteristics (see 48,82,107), their effects on ionic permeability certainly deserve further investigation. Other studies, attempting the incorporation of detergent-solubilized, purified AChR (of varying degrees of purity) into black lipid membranes, have been reviewed recently (26).…”

Section: Reconstitution Of Receptor Systemsmentioning

confidence: 52%

Endogenous Chemical Receptors: Some Physical Aspects

Barrantes¹

1979

Annu. Rev. Biophys. Bioeng.

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Chemical receptors are the mediators of specifi c reactions in a variety of biological processes that range from elementary chemosensory activities in primitive bacteria to the highly sophisticated behavioral mechanisms in man. They have the capacity to specifi cally recognize a given chemical and to generate the requisite signal for evolving a biological response. Strictly speaking, the word "receptor" was used as such for the fi rst time in the early papers of Ehrlich (see 54, 137) (in the discipline now known as immunology), although it was in the context of physiology and pharma cology that it really acquired its "wholistic" connotation. Since then the term has been subjected to considerable use and misuse, often leading to confusion, but above all depriving receptors in many instances of their most superb integrating property. It cannot be over-emphasized that spe cific recognition of a chemical does not suffice to define a receptor [for a clear distinction between receptors and acceptors see (2 1)]. The chemical message encoded in a ligand, say a neurotransmitter or a hormone, is virtually meaningless until it is decoded by its corresponding receptor into purposeful regulatory signals in the target cell. Therefore I adhere throughout this review to the early interpretation of the term, using it to denote both the capacity to specifi cally recognize a ligand (cognitive or discriminatory property) and the capacity to initiate the chain of events leading to the biological effect (gating property).I have not attempted to cover the whole fi eld of chemical receptors, but have preferred instead to restrict the discussion to some endogenous receptors fo r hormones and neurotransmitters. This necessarily implies the omission of several important problems in chemoreception. Arbitrary though it may seem, the review progressively concentrates on a single archetypal system, the receptor fo r the neurotransmitter acetylcholine 287 0084-6589/7910615-0287$0 1.00 Annu. Rev. Biophys. Bioeng. 1979.8:287-321. Downloaded from www.annualreviews.org Access provided by State University of New York -Brooklyn on 03/30/15. For personal use only. Quick links to online content Further ANNUAL REVIEWS

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Section: Reconstitution Of Receptor Systemsmentioning

confidence: 52%

Endogenous Chemical Receptors: Some Physical Aspects

Barrantes¹

1979

Annu. Rev. Biophys. Bioeng.

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“…Even studies in mice with triple knockout of the opioid receptors were conducted in a way that did not allow examination of these nonstereoselective but specific saturable binding sites because of the nature of the control methods (Simonin et al, 2001;Clarke et al, 2002;Kieffer and Gavériaux-Ruff, 2002). Even subsequent studies conducted in Goldstein's laboratory ceased to report the saturable but nonstereoselective binding sites (Lowney et al, 1974). Delfs et al (1994) conducted one of the only studies that provided an opportunity to examine the nonclassic opioid binding sites by using parallel in situ labeling of opioid receptor mRNA and the binding of tritiated naloxone.…”

Section: A What Have We Learned From Several Millennia Ofmentioning

confidence: 99%

Exploring the Neuroimmunopharmacology of Opioids: An Integrative Review of Mechanisms of Central Immune Signaling and Their Implications for Opioid Analgesia

Hutchinson

Shavit

Grace³

et al. 2011

Pharmacol Rev

343

315

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“…It exhibits a high affinity for and stereoselective binding to narcotic drugs; the binding is highly correlated wfth the rank order of analgesic potency for fhese drugs in man and rodent (5). A partially purified opiate receptor isolated from mouse brain (6) was identified as CS (7). A reduction in the availability of brain CS induced pharmacologically or genetically results in a decrease in the analgesic response to morphine (8).…”

mentioning

confidence: 99%

Antibodies to Cerebroside Sulfate Inhibit the Effects of Morphine and β-Endorphin

Craves

Zalc

Leybin

et al. 1980

Science

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Morphine and beta-endorphin inhibit the shaking response of pentobarbital-anesthetized rats to ice water. Stereotaxically guided administration of antibodies to cerebroside sulfate into the periaqueductal gray region, the most sensitive brain region in which to demonstrate inhibition of this response, antagonizes the effect of morphine and beta-endorphin. These results suggest that cerebroside sulfate may be an integral component of an opiate receptor in rat brain.

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Partial Purification of an Opiate Receptor from Mouse Brain

Cited by 90 publications

References 23 publications

Endogenous Chemical Receptors: Some Physical Aspects

Endogenous Chemical Receptors: Some Physical Aspects

Exploring the Neuroimmunopharmacology of Opioids: An Integrative Review of Mechanisms of Central Immune Signaling and Their Implications for Opioid Analgesia

Antibodies to Cerebroside Sulfate Inhibit the Effects of Morphine and β-Endorphin

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