Partial Purification and Chemical Characterization of a Bioemulsifier and Its Application in MEOR

Abstract

The bioemulsifier secreted by strain XS2, which was isolated from oil-contaminated soil samples in Yumen oil field, China, was partially purified and analyzed. Protease hydrolysis and heat treatment results showed that the protein composition of the bioemulsifier played a significant role in emulsification activity. High-performance liquid chromatography (HPLC) study suggested that the protein composition may exist as glycoprotein. Monosaccharide analysis by gas chromatograph-mass spectrometer (GC-MS) showed that galactose, glucose, xylose, and mannose presented in the bioemulsifier. The emulsification activity of this bioemulsifier was relatively high due to different carbohydrates (n-hexadecane 61 %, liquid paraffin 60 %, Toluene 77 %). Furthermore, single well stimulation trial in Changqing oil field, was done to explore the potential application in Microbial-enhanced oil recovery (MEOR). Up to September 2014, the cumulative increased crude oil production was about 88 T in total 240 days, and the water content of the well-produced liquid decreased from 85 to 25 %. These data illustrated that the bioemulsifier performed a high potential in applications and had important economic values.Keyword Bioemulsifier Á XS2 Á Chemical characterization Á MEOR Á Single well stimulation D. Dong and X. Wang are contributed equally to this work.

Direct micro-electric stimulation alters phenanthrene-degrading metabolic activities of Pseudomonas sp. strain DGYH-12 in modified bioelectrochemical system

“…Identification was carried out through phylogenetic analysis based on the sequencing results of 16S rDNA. The morphological, physiological, and phylogenetic characteristics of the strain were analyzed according to procedures that have been described previously (Dong et al 2015).…”