Partial purification and characterization of a jasmonic acid conjugate cleaving amidohydrolase from the fungus Botryodiplodia theobromae

Abstract

A protein preparation from the mycelium of the tropical pathogenic fungus Botryodiplodia theobromae revealed a novel peptidase activity. This enzyme was capable of cleaving conjugates of jasmonic acid with a-amino acids. The protein was enriched 108-fold by gel filtration, ion exchange and hydrophobic interaction chromatography. The enzyme was found to be a glycoprotein with a molecular mass of about 107 kDa. The amidohydrolase seems to be very specific with regard to (-)-jasmonic acid and a-amino acids with (5)-configuration.

Jasmonates: key players in the plant defence

“…However, an enzyme that will hydrolyse jasmonoyl--amino acid conjugates has been identified in the jasmonate producing fungus, Botryodiplodia theobromae. 83 As well as formation of hydroxylated and glycosylated metabolites already mentioned above (section 2.3) another biologically significant pathway of jasmonate removal has recently been described 84 in Lima bean. Use of [ 2 H 5 ]-labelled jasmonate led to the identification of cis-jasmone 30, as a metabolite (Scheme 5).…”

Amino acid conjugates of jasmonic acid induce jasmonate‐responsive gene expression in barley (Hordeum vulgare L.) leaves

“…Apparently, JA amino acid conjugates occur in cells lacking a cleaving enzyme, or the conjugates are inaccessible for cleavage. The only organism from which a JA conjugate cleaving amidohydrolase could be purified so far, the fungus Botryodiplodia theobromae, does not accumulate JA conjugates [31].…”

Jasmonate Biosynthesis in Arabidopsis thaliana - Enzymes, Products, Regulation

“…JA adenylated by JAR1 is conjugated by this enzyme preferentially to isoleucine (Staswick and Tiryaki, 2004), which corresponds to the preferential occurrence of JA-Ile in different plant species (Kramell et al, 1997). JA-Ile hydrolyzing enzyme activity has been found (Hertel et al, 1997). Although this enzyme has not been cloned so far, hydrolysis and conjugation may sustain JA/JA-Ile homeostasis.…”