Partial purification and characterization of the bacteriocin produced by Propionibacterium jensenii B1264

Abstract

-Thirteen dairy propionibacteria were screened for catalase-insensitive, protease-sensitive inhibition of various Gram-positive and Gram-negative bacteria. Production of bacteriocin-like agents was identified in 8 ofthese cultures. The bacteriocin produced by Propionibacteriumjensenii B 1264 inhibited closely related propionibacteria and lactic acid bacteria and was bactericidal to Lactobacillus delbrueckii subsp. lactis ATCC 4797. It was produced in sodium lactate broth cultures during stationary phase; maximum activity was detected after 10 d of growth but only after IO-fold concentration of culture supematants. The bacteriocin was precipated by ammonium sulphate at 70 % saturation. The ammonium sulphate-concentrated, dialyzed bacteriocin was stable to 0.1-1.0 rnol-Lr' NaCI, 0.1-2.0 % SOS, 4 mol-L:' urea, 100 "C (60 min), pH 2-10 and organic sol vents (methanol, ethanol and isopropanol at 0-50 %). Batch anion exchange chromatography (pH 6.5) of the bacteriocin yielded 101 % activity and 15-fold increased purity. Flat bed analytical isoelectric focussing showed that the isoelectric point was between pH 3 and 3.5. SOS-PAGE yielded an estimated molecular mass between 6 and 9 kOa. The evidence suggests that this anionic bacteriocin is unique. © Inra/Eisevier, Paris.

Bacteriocins of propionic acid bacteria

“…Ratman et al [33] also reported that extracellular bacteriocin could only be detected in the late stationary phase of P. jensenii B1264. These researchers suggested that cell lysis occurred and that the bacteriocin was released during lysis.…”

“…In a later screening this strain along with other lactobacilli and PAB were used as indicators [33]. Eight of the thirteen strains tested positive against at least one indicator, but three of them were only active against lactobacilli.…”

“…Interestingly, mild protease treatment stimulated its activity and extended the inhibitory spectrum to more lactobacilli as well as Lactococcus lactis [33].…”

“…By doing this test Ratnam et al [33] discovered that some of the inhibitory zones became larger upon protease treatment. Futhermore, the inhibitory spectrum of P. jensenii B1264 was broadened by protease treatment.…”

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

“…Among them it could be mentioned: Propionicin PLG-1 and GBZ-1 produced by P. thoenii 127 [81]; Jenseniin G isolated from P. thoenii P126 [82]; Propionicins SM1 and SM2 produced by P. jensenii DF1 [83]; Propionicin T1 synthesized by P. thoenii 419 and LMG2792 [84]; Thoenicin 447 isolated from P. thoenii 447 [85]; Acnecin produced by a strain of P. acnes [86] and several other propionicins [87][88][89].…”