Partial purification and characterization of a thermophilic and alkali-stable laccase of <i>Phoma herbarum</i> isolate KU4 with dye-decolorization efficiency

Debnath, Rinku; Mistry, Prasenjit; Roy, Priyabrata; Roy, B.; Saha, Tanima

doi:10.1080/10826068.2021.1875235

Cited by 13 publications

(7 citation statements)

References 125 publications

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“…Compared to the reported dyes [28], most disperse dye solutions showed very broad absorption bands rather than narrow ones, except for Blue HGL 200%, suggesting that there could be a complex composition of industrial-grade dyestuff, usually supplemented to enhance the color strength and dye fixation [21]. Even though there is a significant number of articles reporting the decolorization of various dyes (mostly acid, basic, direct, and active) by laccase [9,29,30], the description of real dye wastewater or industrial-grade dyes is very scarce, especially for disperse dyes. Relative to simulated dye wastewaters, real textile wastewater or industrial-grade dyes show higher recalcitrance towards decolorization due to the complexity in composition that is usually unfriendly to enzymes.…”

Section: Discussionmentioning

confidence: 95%

Biodecolorization and Ecotoxicity Abatement of Disperse Dye-Production Wastewater Treatment with Pycnoporus Laccase

Wang

Chen

Guan

et al. 2022

IJERPH

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The biological treatment efficiency of dye wastewater using activated sludge (AS) is largely limited to the chromaticity and ecotoxicity of dyestuff. To alleviate this limitation, eleven industrial-grade disperse dyes were obtained from a fiber-dyeing factory, and for the first time, we studied the decolorization and detoxification effects of using the Pycnoporus laccase enzyme. Efficient decolorization was achieved with the following conditions: dye concentration 50 mg/L, 1-hydroxybenzotriazole (HBT) 0.15 mM, temperature 65 °C, pH 4, and laccase 0.33 U/mL. The decolorization rate of disperse dyes, ranging from 51 to 96% in this investigation, was highly dependent on the dye type, concentration, laccase loading, and HBT. The ecotoxicity of dyes was evaluated by studying the germination/growth of wheat seed as well as the respiratory rate of aerobic AS. Laccase treatment mitigated the phytotoxicity of dyes because of the higher wheat germination (e.g., increase of 38% for Black ECT 200%) and growth rate (e.g., increase of 91% for Blue 2BLN 200%). The reduced ecotoxicity of decolorized dye solution towards microorganisms was also confirmed by the finding that the oxygen uptake by aerobic AS was increased relative to that of the untreated samples (e.g., increase of 14 folds for Blue HGL 200%). In addition, the chemical oxygen demand (COD) of decolorized dye solution was slightly lower than that without decolorization during the respiratory test. The experimental results suggest that enzymatic decolorization and detoxification can be potentially used as a pretreatment method for disperse dye wastewater followed by AS treatment.

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Section: Discussionmentioning

confidence: 95%

Biodecolorization and Ecotoxicity Abatement of Disperse Dye-Production Wastewater Treatment with Pycnoporus Laccase

Wang

Chen

Guan

et al. 2022

IJERPH

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“…Earlier, we found that (Debnath et al . 2021) rice straw and saw dust significantly enhanced the laccase production in SmF (1590·22 U ml −1 ) as well as SSF (2014·21 U ml −1 or 12 085·26 U g −1 ). To achieve maximum enzyme production, in the present study, we investigated the laccase production using RSM.…”

Section: Introductionmentioning

confidence: 86%

“…herbarum isolate KU4 was isolated from wood and leaf decaying soils of University of Kalyani, West Bengal, India (Debnath et al . 2021). The fungus was maintained on potato dextrose agar slants at 12°C.…”

Section: Methodsmentioning

confidence: 99%

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Enrichment of laccase production byPhoma herbarumisolate KU4 under solid‐state fermentation by optimizing RSM coefficients using genetic algorithm

Debnath

Das

Mukhopadhyay

et al. 2021

Lett Appl Microbiol

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The process parameters were optimized to obtain enhanced enzyme activity from the fungus Phoma herbarum isolate KU4 using rice straw and saw dust as substrate under solid‐state fermentation using Response surface methodology (RSM). Genetic algorithm was used to validate the RSM for maximum laccase production. Six variables, viz., pH of the media, initial moisture content, copper sulphate concentration, concentration of tannic acid, inoculum concentration and incubation time were found to be effective and optimized for enhanced production. Maximum laccase production was achieved by RSM at pH 5·0 and 86% of initial moisture content of the culture medium, 150 µmol l−1 of CuSO4, 1·5% tannic acid and 0·128 g inoculum g−1 dry substrate inoculum size on the fourth day of fermentation. The highest laccase activity was observed as 79 008 U g−1, which is approximately sixfold enhanced production compared to the unoptimized condition (12 085·26 U g−1).

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“…The first laccase was obtained from a Japanese lacquer tree (Rhus vernicifera), since then new fungal laccases from Trametes versicolor, Polyporus pinsitus, Rhizocotonia solani and from Ascomycetes Myceliophthora thermophila and Sccytalidium thermophilum etc. were obtained and studied extensively [142,143,[145][146][147][148][149][150][151][152][153][154][155][156][157][158][159].…”

Section: Biocomposites From Fibres Using Enzyme and Ligninmentioning

confidence: 99%

Opportunity of Non-Wood Forest Products in Biocomposites

Sharma¹

2022

Biocomposites

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In recent years industries are attempting to decrease their dependency on petroleum-based fuels and products due to increased environmental issues. The tremendous increase in production and use of plastics in every sector of life has led to huge plastic waste disposal problems and also an environmental threat. In order to prevail over the present scenario, the viable and cost-effective approaches are to prepare eco-friendly bio-composites based on non-wood forest products (NWFP), a part of forest wealth of the globe, especially natural fibres, agricultural wastes and extractives. Natural fibres and extractives have many advantages viz. low density, low cost, considerable toughness properties, nontoxicity, sustainability and biodegradability. NWFP based composites may be utilized to produce non-structural parts for diverse applications in various industries as high-performance materials with interesting properties for specific applications viz. furniture, thermal, acoustic insulations and automotive industries etc. In the present chapter, opportunities of extractives, cellulosic and lignocellulosic fibres from non-wood forest products in Bio-composites will be discussed.

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Partial purification and characterization of a thermophilic and alkali-stable laccase of Phoma herbarum isolate KU4 with dye-decolorization efficiency

Cited by 13 publications

References 125 publications

Biodecolorization and Ecotoxicity Abatement of Disperse Dye-Production Wastewater Treatment with Pycnoporus Laccase

Biodecolorization and Ecotoxicity Abatement of Disperse Dye-Production Wastewater Treatment with Pycnoporus Laccase

Enrichment of laccase production byPhoma herbarumisolate KU4 under solid‐state fermentation by optimizing RSM coefficients using genetic algorithm

Opportunity of Non-Wood Forest Products in Biocomposites

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